Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2599 and ribA are separated by 114 nucleotides ribA and Shewana3_2601 are separated by 263 nucleotides Shewana3_2601 and nrdG are separated by 125 nucleotides nrdG and Shewana3_2603 are separated by 105 nucleotides
Shewana3_2599: Shewana3_2599 - hypothetical protein (RefSeq), at 3,103,115 to 3,103,984
_2599
Shewana3_2600: ribA - GTP cyclohydrolase II (RefSeq), at 3,104,099 to 3,104,710
ribA
Shewana3_2601: Shewana3_2601 - hypothetical protein (RefSeq), at 3,104,974 to 3,105,330
_2601
Shewana3_2602: nrdG - anaerobic ribonucleotide reductase-activating protein (RefSeq), at 3,105,456 to 3,105,917
nrdG
Shewana3_2603: Shewana3_2603 - anaerobic ribonucleoside triphosphate reductase (RefSeq), at 3,106,023 to 3,108,140
_2603
Position (kb)
3104
3105
3106 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3104.883 kb on - strand at 3105.109 kb on - strand, within Shewana3_2601 at 3105.114 kb on - strand, within Shewana3_2601 at 3105.138 kb on - strand, within Shewana3_2601 at 3105.226 kb on - strand, within Shewana3_2601 at 3105.602 kb on - strand, within nrdG at 3105.667 kb on + strand, within nrdG at 3105.668 kb on + strand, within nrdG at 3105.812 kb on + strand, within nrdG at 3105.859 kb on - strand, within nrdG at 3106.129 kb on - strand at 3106.286 kb on + strand, within Shewana3_2603 at 3106.286 kb on + strand, within Shewana3_2603 at 3106.301 kb on - strand, within Shewana3_2603
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 3,104,883 - -0.5 3,105,109 - Shewana3_2601 0.38 +0.6 3,105,114 - Shewana3_2601 0.39 +1.9 3,105,138 - Shewana3_2601 0.46 +1.4 3,105,226 - Shewana3_2601 0.71 +0.5 3,105,602 - nrdG Shewana3_2602 0.32 +0.3 3,105,667 + nrdG Shewana3_2602 0.46 -1.6 3,105,668 + nrdG Shewana3_2602 0.46 -2.0 3,105,812 + nrdG Shewana3_2602 0.77 +0.0 3,105,859 - nrdG Shewana3_2602 0.87 +0.8 3,106,129 - +0.3 3,106,286 + Shewana3_2603 0.12 +1.1 3,106,286 + Shewana3_2603 0.12 -0.8 3,106,301 - Shewana3_2603 0.13 -0.7
Or see this region's nucleotide sequence