Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2539 and Shewana3_2540 are separated by 142 nucleotides Shewana3_2540 and Shewana3_2541 are separated by 74 nucleotides
Shewana3_2539: Shewana3_2539 - OmpA/MotB domain-containing protein (RefSeq), at 3,036,008 to 3,036,880
_2539
Shewana3_2540: Shewana3_2540 - ribonuclease T (RefSeq), at 3,037,023 to 3,037,691
_2540
Shewana3_2541: Shewana3_2541 - alkyl hydroperoxide reductase/Thiol specific antioxidant/Mal allergen (RefSeq), at 3,037,766 to 3,038,371
_2541
Position (kb)
3037
3038 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3036.044 kb on + strand at 3036.103 kb on + strand, within Shewana3_2539 at 3036.125 kb on - strand, within Shewana3_2539 at 3036.541 kb on - strand, within Shewana3_2539 at 3036.541 kb on - strand, within Shewana3_2539 at 3036.613 kb on - strand, within Shewana3_2539 at 3036.613 kb on - strand, within Shewana3_2539 at 3036.631 kb on - strand, within Shewana3_2539 at 3036.645 kb on - strand, within Shewana3_2539 at 3036.805 kb on + strand at 3037.106 kb on + strand, within Shewana3_2540 at 3037.206 kb on + strand, within Shewana3_2540 at 3037.572 kb on + strand, within Shewana3_2540 at 3037.728 kb on + strand at 3037.820 kb on + strand at 3037.984 kb on - strand, within Shewana3_2541 at 3038.166 kb on - strand, within Shewana3_2541 at 3038.171 kb on - strand, within Shewana3_2541 at 3038.192 kb on - strand, within Shewana3_2541 at 3038.305 kb on - strand, within Shewana3_2541 at 3038.315 kb on + strand at 3038.323 kb on - strand at 3038.576 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 3,036,044 + +1.0 3,036,103 + Shewana3_2539 0.11 -0.7 3,036,125 - Shewana3_2539 0.13 +1.5 3,036,541 - Shewana3_2539 0.61 +1.6 3,036,541 - Shewana3_2539 0.61 +1.2 3,036,613 - Shewana3_2539 0.69 +1.9 3,036,613 - Shewana3_2539 0.69 -0.5 3,036,631 - Shewana3_2539 0.71 +1.9 3,036,645 - Shewana3_2539 0.73 +0.8 3,036,805 + +2.3 3,037,106 + Shewana3_2540 0.12 +2.3 3,037,206 + Shewana3_2540 0.27 +1.3 3,037,572 + Shewana3_2540 0.82 -0.1 3,037,728 + -0.3 3,037,820 + +1.3 3,037,984 - Shewana3_2541 0.36 +2.6 3,038,166 - Shewana3_2541 0.66 +1.3 3,038,171 - Shewana3_2541 0.67 +1.9 3,038,192 - Shewana3_2541 0.70 +0.7 3,038,305 - Shewana3_2541 0.89 +2.3 3,038,315 + +1.3 3,038,323 - +1.8 3,038,576 - -2.1
Or see this region's nucleotide sequence