Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2420 and Shewana3_2421 are separated by 81 nucleotides Shewana3_2421 and Shewana3_2422 are separated by 235 nucleotides Shewana3_2422 and Shewana3_2423 are separated by 81 nucleotides
Shewana3_2420: Shewana3_2420 - hypothetical protein (RefSeq), at 2,903,581 to 2,904,141
_2420
Shewana3_2421: Shewana3_2421 - hypothetical protein (RefSeq), at 2,904,223 to 2,904,831
_2421
Shewana3_2422: Shewana3_2422 - hypothetical protein (RefSeq), at 2,905,067 to 2,905,327
_2422
Shewana3_2423: Shewana3_2423 - peptidase M23B (RefSeq), at 2,905,409 to 2,906,329
_2423
Position (kb)
2905
2906 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2904.468 kb on - strand, within Shewana3_2421 at 2904.542 kb on + strand, within Shewana3_2421 at 2904.608 kb on - strand, within Shewana3_2421 at 2904.722 kb on - strand, within Shewana3_2421 at 2904.797 kb on + strand at 2904.868 kb on - strand at 2904.876 kb on - strand at 2904.968 kb on + strand at 2905.036 kb on + strand at 2905.152 kb on + strand, within Shewana3_2422 at 2905.185 kb on - strand, within Shewana3_2422 at 2905.272 kb on + strand, within Shewana3_2422 at 2905.641 kb on + strand, within Shewana3_2423 at 2905.660 kb on + strand, within Shewana3_2423 at 2905.915 kb on - strand, within Shewana3_2423 at 2906.004 kb on - strand, within Shewana3_2423 at 2906.230 kb on - strand, within Shewana3_2423
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 2,904,468 - Shewana3_2421 0.40 -1.5 2,904,542 + Shewana3_2421 0.52 -2.5 2,904,608 - Shewana3_2421 0.63 -1.7 2,904,722 - Shewana3_2421 0.82 +0.1 2,904,797 + -2.2 2,904,868 - +0.7 2,904,876 - +2.3 2,904,968 + -0.1 2,905,036 + +0.7 2,905,152 + Shewana3_2422 0.33 -2.9 2,905,185 - Shewana3_2422 0.45 +0.7 2,905,272 + Shewana3_2422 0.79 +0.4 2,905,641 + Shewana3_2423 0.25 -0.4 2,905,660 + Shewana3_2423 0.27 +0.7 2,905,915 - Shewana3_2423 0.55 +0.7 2,906,004 - Shewana3_2423 0.65 +0.4 2,906,230 - Shewana3_2423 0.89 +0.6
Or see this region's nucleotide sequence