Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2376 and Shewana3_2377 are separated by 45 nucleotides Shewana3_2377 and Shewana3_2378 are separated by 190 nucleotides Shewana3_2378 and Shewana3_2379 are separated by 167 nucleotides Shewana3_2379 and Shewana3_2380 are separated by 38 nucleotides
Shewana3_2376: Shewana3_2376 - DEAD/DEAH box helicase domain-containing protein (RefSeq), at 2,855,888 to 2,857,363
_2376
Shewana3_2377: Shewana3_2377 - peptidyl-prolyl cis-trans isomerase B (RefSeq), at 2,857,409 to 2,857,909
_2377
Shewana3_2378: Shewana3_2378 - iron-containing alcohol dehydrogenase (RefSeq), at 2,858,100 to 2,859,713
_2378
Shewana3_2379: Shewana3_2379 - hypothetical protein (RefSeq), at 2,859,881 to 2,860,339
_2379
Shewana3_2380: Shewana3_2380 - putative transglycosylase (RefSeq), at 2,860,378 to 2,861,379
_2380
Position (kb)
2858
2859
2860 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2857.151 kb on - strand, within Shewana3_2376 at 2857.274 kb on - strand at 2857.544 kb on + strand, within Shewana3_2377 at 2859.793 kb on + strand at 2859.988 kb on - strand, within Shewana3_2379 at 2860.140 kb on + strand, within Shewana3_2379 at 2860.148 kb on - strand, within Shewana3_2379 at 2860.215 kb on - strand, within Shewana3_2379 at 2860.254 kb on + strand, within Shewana3_2379 at 2860.453 kb on - strand at 2860.589 kb on - strand, within Shewana3_2380 at 2860.589 kb on - strand, within Shewana3_2380
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 2,857,151 - Shewana3_2376 0.86 +1.3 2,857,274 - -0.8 2,857,544 + Shewana3_2377 0.27 +0.9 2,859,793 + -0.2 2,859,988 - Shewana3_2379 0.23 +1.3 2,860,140 + Shewana3_2379 0.56 +0.0 2,860,148 - Shewana3_2379 0.58 +0.5 2,860,215 - Shewana3_2379 0.73 -1.2 2,860,254 + Shewana3_2379 0.81 +0.0 2,860,453 - -1.6 2,860,589 - Shewana3_2380 0.21 +0.5 2,860,589 - Shewana3_2380 0.21 -2.9
Or see this region's nucleotide sequence