Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2334 and Shewana3_2335 are separated by 78 nucleotides Shewana3_2335 and Shewana3_2336 overlap by 8 nucleotides Shewana3_2336 and Shewana3_2337 are separated by 95 nucleotides Shewana3_2337 and Shewana3_2338 are separated by 244 nucleotides
Shewana3_2334: Shewana3_2334 - protein tyrosine phosphatase (RefSeq), at 2,809,813 to 2,810,319
_2334
Shewana3_2335: Shewana3_2335 - ArsR family transcriptional regulator (RefSeq), at 2,810,398 to 2,810,745
_2335
Shewana3_2336: Shewana3_2336 - protein tyrosine phosphatase (RefSeq), at 2,810,738 to 2,811,154
_2336
Shewana3_2337: Shewana3_2337 - ArsR family transcriptional regulator (RefSeq), at 2,811,250 to 2,811,537
_2337
Shewana3_2338: Shewana3_2338 - permease (RefSeq), at 2,811,782 to 2,812,858
_2338
Position (kb)
2811
2812 Strain fitness (log2 ratio)
-1
0
1
2 at 2810.667 kb on - strand, within Shewana3_2335 at 2810.743 kb on - strand at 2810.804 kb on + strand, within Shewana3_2336 at 2810.855 kb on - strand, within Shewana3_2336 at 2811.036 kb on + strand at 2811.244 kb on - strand at 2811.417 kb on - strand, within Shewana3_2337 at 2811.485 kb on - strand, within Shewana3_2337 at 2811.629 kb on + strand at 2811.656 kb on - strand at 2811.848 kb on - strand at 2811.949 kb on + strand, within Shewana3_2338 at 2812.157 kb on + strand, within Shewana3_2338 at 2812.228 kb on + strand, within Shewana3_2338
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 2,810,667 - Shewana3_2335 0.77 +1.1 2,810,743 - +1.2 2,810,804 + Shewana3_2336 0.16 +1.4 2,810,855 - Shewana3_2336 0.28 +1.4 2,811,036 + +2.4 2,811,244 - +0.8 2,811,417 - Shewana3_2337 0.58 +0.8 2,811,485 - Shewana3_2337 0.82 +2.4 2,811,629 + -0.2 2,811,656 - -1.1 2,811,848 - -0.6 2,811,949 + Shewana3_2338 0.16 +0.8 2,812,157 + Shewana3_2338 0.35 +0.4 2,812,228 + Shewana3_2338 0.41 -0.2
Or see this region's nucleotide sequence