Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2231 and Shewana3_2232 are separated by 703 nucleotides Shewana3_2232 and Shewana3_2233 are separated by 91 nucleotides Shewana3_2233 and Shewana3_2234 are separated by 111 nucleotides
Shewana3_2231: Shewana3_2231 - cation diffusion facilitator family transporter (RefSeq), at 2,682,753 to 2,684,003
_2231
Shewana3_2232: Shewana3_2232 - lactoylglutathione lyase (RefSeq), at 2,684,707 to 2,685,117
_2232
Shewana3_2233: Shewana3_2233 - putative sulfite oxidase subunit YedZ (RefSeq), at 2,685,209 to 2,685,856
_2233
Shewana3_2234: Shewana3_2234 - putative sulfite oxidase subunit YedY (RefSeq), at 2,685,968 to 2,687,002
_2234
Position (kb)
2684
2685
2686 Strain fitness (log2 ratio)
-1
0
1
2 at 2683.826 kb on - strand, within Shewana3_2231 at 2683.958 kb on + strand at 2683.966 kb on - strand at 2683.966 kb on - strand at 2684.216 kb on + strand at 2684.405 kb on - strand at 2684.472 kb on - strand at 2684.716 kb on + strand at 2684.731 kb on - strand at 2685.112 kb on + strand at 2685.114 kb on - strand at 2685.206 kb on + strand at 2685.402 kb on - strand, within Shewana3_2233 at 2685.616 kb on + strand, within Shewana3_2233 at 2685.810 kb on - strand at 2686.034 kb on - strand at 2686.035 kb on - strand at 2686.096 kb on + strand, within Shewana3_2234
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 2,683,826 - Shewana3_2231 0.86 +0.8 2,683,958 + +0.9 2,683,966 - +0.2 2,683,966 - +0.9 2,684,216 + +0.8 2,684,405 - +1.6 2,684,472 - +0.5 2,684,716 + +0.5 2,684,731 - +1.8 2,685,112 + +0.2 2,685,114 - +2.4 2,685,206 + +1.4 2,685,402 - Shewana3_2233 0.30 -0.2 2,685,616 + Shewana3_2233 0.63 +0.8 2,685,810 - -1.0 2,686,034 - -0.6 2,686,035 - -0.4 2,686,096 + Shewana3_2234 0.12 -1.2
Or see this region's nucleotide sequence