Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2110 and Shewana3_2111 are separated by 110 nucleotides Shewana3_2111 and Shewana3_2112 are separated by 10 nucleotides
Shewana3_2110: Shewana3_2110 - cytochrome c oxidase, cbb3-type, subunit III (RefSeq), at 2,533,845 to 2,534,813
_2110
Shewana3_2111: Shewana3_2111 - hypothetical protein (RefSeq), at 2,534,924 to 2,535,403
_2111
Shewana3_2112: Shewana3_2112 - heavy metal translocating P-type ATPase (RefSeq), at 2,535,414 to 2,537,825
_2112
Position (kb)
2534
2535
2536 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 2533.924 kb on + strand at 2533.972 kb on + strand, within Shewana3_2110 at 2534.067 kb on - strand, within Shewana3_2110 at 2534.113 kb on + strand, within Shewana3_2110 at 2534.113 kb on + strand, within Shewana3_2110 at 2534.353 kb on - strand, within Shewana3_2110 at 2534.388 kb on - strand, within Shewana3_2110 at 2534.528 kb on + strand, within Shewana3_2110 at 2534.596 kb on + strand, within Shewana3_2110 at 2535.108 kb on - strand, within Shewana3_2111 at 2535.166 kb on - strand, within Shewana3_2111 at 2535.168 kb on - strand, within Shewana3_2111 at 2535.168 kb on - strand, within Shewana3_2111 at 2535.290 kb on - strand, within Shewana3_2111 at 2535.299 kb on + strand, within Shewana3_2111 at 2535.687 kb on + strand, within Shewana3_2112 at 2535.756 kb on - strand, within Shewana3_2112 at 2536.380 kb on + strand, within Shewana3_2112
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 2,533,924 + +4.7 2,533,972 + Shewana3_2110 0.13 +4.9 2,534,067 - Shewana3_2110 0.23 +2.4 2,534,113 + Shewana3_2110 0.28 +5.3 2,534,113 + Shewana3_2110 0.28 +5.1 2,534,353 - Shewana3_2110 0.52 +4.1 2,534,388 - Shewana3_2110 0.56 +4.0 2,534,528 + Shewana3_2110 0.70 +4.9 2,534,596 + Shewana3_2110 0.78 +4.9 2,535,108 - Shewana3_2111 0.38 +4.0 2,535,166 - Shewana3_2111 0.50 +4.0 2,535,168 - Shewana3_2111 0.51 +3.8 2,535,168 - Shewana3_2111 0.51 +3.3 2,535,290 - Shewana3_2111 0.76 +3.5 2,535,299 + Shewana3_2111 0.78 +4.0 2,535,687 + Shewana3_2112 0.11 +4.8 2,535,756 - Shewana3_2112 0.14 +1.8 2,536,380 + Shewana3_2112 0.40 +4.3
Or see this region's nucleotide sequence