Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2081 and Shewana3_2082 are separated by 208 nucleotides Shewana3_2082 and Shewana3_2083 overlap by 1 nucleotides
Shewana3_2081: Shewana3_2081 - sugar (glycoside-Pentoside-hexuronide) transporter (RefSeq), at 2,491,773 to 2,493,218
_2081
Shewana3_2082: Shewana3_2082 - arabinan endo-1,5-alpha-L-arabinosidase (RefSeq), at 2,493,427 to 2,494,497
_2082
Shewana3_2083: Shewana3_2083 - glycoside hydrolase family protein (RefSeq), at 2,494,497 to 2,496,401
_2083
Position (kb)
2493
2494
2495 Strain fitness (log2 ratio)
-1
0
1
2 at 2492.476 kb on - strand, within Shewana3_2081 at 2492.572 kb on + strand, within Shewana3_2081 at 2492.929 kb on + strand, within Shewana3_2081 at 2493.767 kb on - strand, within Shewana3_2082 at 2493.973 kb on + strand, within Shewana3_2082 at 2493.974 kb on + strand, within Shewana3_2082 at 2494.192 kb on + strand, within Shewana3_2082 at 2494.265 kb on - strand, within Shewana3_2082 at 2494.432 kb on - strand at 2494.436 kb on + strand at 2494.436 kb on + strand at 2494.436 kb on + strand at 2494.451 kb on + strand at 2494.577 kb on + strand at 2494.585 kb on - strand at 2494.585 kb on - strand at 2495.041 kb on - strand, within Shewana3_2083 at 2495.099 kb on + strand, within Shewana3_2083 at 2495.119 kb on + strand, within Shewana3_2083 at 2495.126 kb on + strand, within Shewana3_2083 at 2495.357 kb on - strand, within Shewana3_2083
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 2,492,476 - Shewana3_2081 0.49 +0.0 2,492,572 + Shewana3_2081 0.55 +2.5 2,492,929 + Shewana3_2081 0.80 +1.5 2,493,767 - Shewana3_2082 0.32 +0.5 2,493,973 + Shewana3_2082 0.51 -1.2 2,493,974 + Shewana3_2082 0.51 -0.7 2,494,192 + Shewana3_2082 0.71 +0.1 2,494,265 - Shewana3_2082 0.78 +1.5 2,494,432 - +1.5 2,494,436 + +1.0 2,494,436 + +0.5 2,494,436 + -0.5 2,494,451 + -0.8 2,494,577 + -0.7 2,494,585 - +1.5 2,494,585 - +0.5 2,495,041 - Shewana3_2083 0.29 +1.1 2,495,099 + Shewana3_2083 0.32 -0.5 2,495,119 + Shewana3_2083 0.33 -0.7 2,495,126 + Shewana3_2083 0.33 +0.1 2,495,357 - Shewana3_2083 0.45 +0.9
Or see this region's nucleotide sequence