Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2047 and Shewana3_2048 are separated by 250 nucleotides Shewana3_2048 and Shewana3_2049 are separated by 180 nucleotides
Shewana3_2047: Shewana3_2047 - thioredoxin reductase (RefSeq), at 2,454,590 to 2,455,543
_2047
Shewana3_2048: Shewana3_2048 - L-alanine dehydrogenase (RefSeq), at 2,455,794 to 2,456,909
_2048
Shewana3_2049: Shewana3_2049 - leucine-responsive transcriptional regulator (RefSeq), at 2,457,090 to 2,457,596
_2049
Position (kb)
2455
2456
2457 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 2455.093 kb on - strand, within Shewana3_2047 at 2455.175 kb on + strand at 2455.175 kb on + strand, within Shewana3_2047 at 2455.183 kb on - strand, within Shewana3_2047 at 2455.183 kb on - strand, within Shewana3_2047 at 2455.219 kb on + strand, within Shewana3_2047 at 2455.457 kb on - strand at 2455.492 kb on - strand at 2455.584 kb on + strand at 2455.673 kb on - strand at 2456.135 kb on - strand, within Shewana3_2048 at 2456.364 kb on + strand, within Shewana3_2048 at 2456.672 kb on - strand, within Shewana3_2048 at 2456.692 kb on - strand, within Shewana3_2048 at 2456.993 kb on + strand at 2457.004 kb on - strand at 2457.004 kb on - strand at 2457.191 kb on + strand, within Shewana3_2049 at 2457.191 kb on + strand, within Shewana3_2049 at 2457.199 kb on - strand, within Shewana3_2049 at 2457.219 kb on - strand, within Shewana3_2049 at 2457.365 kb on + strand, within Shewana3_2049 at 2457.378 kb on + strand, within Shewana3_2049
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 2,455,093 - Shewana3_2047 0.53 +3.5 2,455,175 + +2.4 2,455,175 + Shewana3_2047 0.61 +1.4 2,455,183 - Shewana3_2047 0.62 +3.7 2,455,183 - Shewana3_2047 0.62 +4.4 2,455,219 + Shewana3_2047 0.66 +2.1 2,455,457 - +4.1 2,455,492 - +5.7 2,455,584 + +3.4 2,455,673 - +1.4 2,456,135 - Shewana3_2048 0.31 +2.2 2,456,364 + Shewana3_2048 0.51 +1.4 2,456,672 - Shewana3_2048 0.79 +0.8 2,456,692 - Shewana3_2048 0.80 +0.3 2,456,993 + +0.2 2,457,004 - -0.3 2,457,004 - +0.6 2,457,191 + Shewana3_2049 0.20 +2.3 2,457,191 + Shewana3_2049 0.20 +2.0 2,457,199 - Shewana3_2049 0.21 -0.2 2,457,219 - Shewana3_2049 0.25 -1.3 2,457,365 + Shewana3_2049 0.54 +1.8 2,457,378 + Shewana3_2049 0.57 +2.2
Or see this region's nucleotide sequence