Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvB and Shewana3_1958 are separated by 974 nucleotides Shewana3_1958 and Shewana3_1959 are separated by 197 nucleotides
Shewana3_1957: ruvB - Holliday junction DNA helicase RuvB (RefSeq), at 2,328,348 to 2,329,352
ruvB
Shewana3_1958: Shewana3_1958 - TonB-dependent receptor (RefSeq), at 2,330,327 to 2,332,888
_1958
Shewana3_1959: Shewana3_1959 - two component transcriptional regulator (RefSeq), at 2,333,086 to 2,333,766
_1959
Position (kb)
2330
2331
2332
2333 Strain fitness (log2 ratio)
-1
0
1
2 at 2329.342 kb on + strand at 2329.526 kb on + strand at 2329.700 kb on + strand at 2330.537 kb on + strand at 2330.582 kb on - strand at 2330.737 kb on - strand, within Shewana3_1958 at 2330.787 kb on + strand, within Shewana3_1958 at 2330.922 kb on - strand, within Shewana3_1958 at 2331.330 kb on + strand, within Shewana3_1958 at 2331.706 kb on - strand, within Shewana3_1958 at 2332.185 kb on - strand, within Shewana3_1958 at 2332.301 kb on - strand, within Shewana3_1958 at 2332.438 kb on - strand, within Shewana3_1958 at 2332.547 kb on + strand, within Shewana3_1958 at 2332.758 kb on + strand at 2332.835 kb on + strand at 2333.000 kb on - strand at 2333.194 kb on - strand, within Shewana3_1959 at 2333.213 kb on + strand, within Shewana3_1959 at 2333.291 kb on + strand, within Shewana3_1959 at 2333.432 kb on + strand, within Shewana3_1959 at 2333.432 kb on + strand, within Shewana3_1959 at 2333.705 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 2,329,342 + +0.4 2,329,526 + -0.4 2,329,700 + +0.4 2,330,537 + +0.3 2,330,582 - -0.7 2,330,737 - Shewana3_1958 0.16 +2.4 2,330,787 + Shewana3_1958 0.18 +0.1 2,330,922 - Shewana3_1958 0.23 +1.4 2,331,330 + Shewana3_1958 0.39 +2.4 2,331,706 - Shewana3_1958 0.54 +2.4 2,332,185 - Shewana3_1958 0.73 +2.4 2,332,301 - Shewana3_1958 0.77 +0.9 2,332,438 - Shewana3_1958 0.82 +0.9 2,332,547 + Shewana3_1958 0.87 +1.9 2,332,758 + +0.2 2,332,835 + +1.1 2,333,000 - +1.9 2,333,194 - Shewana3_1959 0.16 +2.4 2,333,213 + Shewana3_1959 0.19 -0.2 2,333,291 + Shewana3_1959 0.30 +0.4 2,333,432 + Shewana3_1959 0.51 +2.4 2,333,432 + Shewana3_1959 0.51 +1.4 2,333,705 + +1.4
Or see this region's nucleotide sequence