Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1922 and Shewana3_1923 are separated by 79 nucleotides Shewana3_1923 and Shewana3_1924 are separated by 232 nucleotides
Shewana3_1922: Shewana3_1922 - sodium/sulphate symporter (RefSeq), at 2,284,710 to 2,286,098
_1922
Shewana3_1923: Shewana3_1923 - AraC family transcriptional regulator (RefSeq), at 2,286,178 to 2,286,990
_1923
Shewana3_1924: Shewana3_1924 - glucan 1,4-alpha-glucosidase (RefSeq), at 2,287,223 to 2,289,736
_1924
Position (kb)
2286
2287 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2285.237 kb on + strand, within Shewana3_1922 at 2285.460 kb on - strand, within Shewana3_1922 at 2285.511 kb on + strand, within Shewana3_1922 at 2285.984 kb on - strand at 2286.405 kb on + strand, within Shewana3_1923 at 2286.490 kb on + strand, within Shewana3_1923 at 2286.490 kb on + strand, within Shewana3_1923 at 2286.498 kb on - strand, within Shewana3_1923 at 2286.502 kb on - strand, within Shewana3_1923 at 2286.534 kb on - strand, within Shewana3_1923 at 2286.617 kb on + strand, within Shewana3_1923 at 2286.630 kb on + strand, within Shewana3_1923 at 2286.847 kb on - strand, within Shewana3_1923 at 2286.877 kb on - strand, within Shewana3_1923 at 2286.922 kb on + strand at 2287.086 kb on - strand at 2287.111 kb on + strand at 2287.199 kb on + strand at 2287.406 kb on + strand at 2287.437 kb on - strand at 2287.587 kb on - strand, within Shewana3_1924 at 2287.666 kb on - strand, within Shewana3_1924 at 2287.825 kb on - strand, within Shewana3_1924 at 2287.945 kb on - strand, within Shewana3_1924
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 2,285,237 + Shewana3_1922 0.38 +0.5 2,285,460 - Shewana3_1922 0.54 +2.5 2,285,511 + Shewana3_1922 0.58 +0.1 2,285,984 - -1.0 2,286,405 + Shewana3_1923 0.28 -0.5 2,286,490 + Shewana3_1923 0.38 -2.7 2,286,490 + Shewana3_1923 0.38 +0.0 2,286,498 - Shewana3_1923 0.39 -0.9 2,286,502 - Shewana3_1923 0.40 +0.5 2,286,534 - Shewana3_1923 0.44 +0.5 2,286,617 + Shewana3_1923 0.54 +0.1 2,286,630 + Shewana3_1923 0.56 -0.3 2,286,847 - Shewana3_1923 0.82 +1.5 2,286,877 - Shewana3_1923 0.86 +1.5 2,286,922 + -0.5 2,287,086 - +0.9 2,287,111 + -0.2 2,287,199 + -0.1 2,287,406 + +0.5 2,287,437 - +1.2 2,287,587 - Shewana3_1924 0.14 +0.1 2,287,666 - Shewana3_1924 0.18 -0.0 2,287,825 - Shewana3_1924 0.24 +0.6 2,287,945 - Shewana3_1924 0.29 +0.4
Or see this region's nucleotide sequence