Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1662 and Shewana3_1663 are separated by 225 nucleotides Shewana3_1663 and Shewana3_1664 are separated by 35 nucleotides Shewana3_1664 and Shewana3_1665 are separated by 107 nucleotides
Shewana3_1662: Shewana3_1662 - hypothetical protein (RefSeq), at 1,954,074 to 1,954,829
_1662
Shewana3_1663: Shewana3_1663 - hypothetical protein (RefSeq), at 1,955,055 to 1,955,366
_1663
Shewana3_1664: Shewana3_1664 - ThiJ/PfpI domain-containing protein (RefSeq), at 1,955,402 to 1,956,004
_1664
Shewana3_1665: Shewana3_1665 - hypothetical protein (RefSeq), at 1,956,112 to 1,957,143
_1665
Position (kb) 1955
1956
1957 Strain fitness (log2 ratio) -1
0
1
2 at 1954.552 kb on + strand, within Shewana3_1662 at 1954.794 kb on + strand at 1954.990 kb on + strand at 1955.207 kb on - strand, within Shewana3_1663 at 1955.869 kb on + strand, within Shewana3_1664 at 1956.027 kb on + strand at 1956.319 kb on - strand, within Shewana3_1665 at 1956.342 kb on - strand, within Shewana3_1665 at 1956.342 kb on - strand, within Shewana3_1665
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 1,954,552 + Shewana3_1662 0.63 +0.7 1,954,794 + -0.0 1,954,990 + +1.3 1,955,207 - Shewana3_1663 0.49 +0.9 1,955,869 + Shewana3_1664 0.77 -0.6 1,956,027 + +2.3 1,956,319 - Shewana3_1665 0.20 +2.2 1,956,342 - Shewana3_1665 0.22 -0.6 1,956,342 - Shewana3_1665 0.22 -1.0
Or see this region's nucleotide sequence