Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1646 and Shewana3_1647 are separated by 388 nucleotides Shewana3_1647 and Shewana3_1648 are separated by 47 nucleotides Shewana3_1648 and Shewana3_1649 are separated by 111 nucleotides
Shewana3_1646: Shewana3_1646 - hypothetical protein (RefSeq), at 1,936,816 to 1,938,744
_1646
Shewana3_1647: Shewana3_1647 - hypothetical protein (RefSeq), at 1,939,133 to 1,939,513
_1647
Shewana3_1648: Shewana3_1648 - arsenate reductase (RefSeq), at 1,939,561 to 1,939,911
_1648
Shewana3_1649: Shewana3_1649 - peptidase M48, Ste24p (RefSeq), at 1,940,023 to 1,941,492
_1649
Position (kb)
1939
1940 Strain fitness (log2 ratio)
-1
0
1
2 at 1938.597 kb on + strand at 1938.597 kb on + strand at 1938.855 kb on - strand at 1939.078 kb on + strand at 1939.162 kb on + strand at 1939.312 kb on + strand, within Shewana3_1647 at 1939.394 kb on - strand, within Shewana3_1647 at 1939.409 kb on + strand, within Shewana3_1647 at 1939.422 kb on + strand, within Shewana3_1647 at 1939.465 kb on + strand, within Shewana3_1647 at 1939.500 kb on + strand at 1939.851 kb on + strand, within Shewana3_1648 at 1939.851 kb on + strand, within Shewana3_1648 at 1940.112 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 1,938,597 + +0.8 1,938,597 + +0.2 1,938,855 - +0.2 1,939,078 + +1.2 1,939,162 + -0.1 1,939,312 + Shewana3_1647 0.47 -0.8 1,939,394 - Shewana3_1647 0.69 +1.8 1,939,409 + Shewana3_1647 0.72 +0.2 1,939,422 + Shewana3_1647 0.76 -0.1 1,939,465 + Shewana3_1647 0.87 -1.0 1,939,500 + -0.3 1,939,851 + Shewana3_1648 0.83 -1.6 1,939,851 + Shewana3_1648 0.83 -1.0 1,940,112 - +0.5
Or see this region's nucleotide sequence