Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1620 and Shewana3_1621 overlap by 4 nucleotides Shewana3_1621 and Shewana3_1622 are separated by 87 nucleotides Shewana3_1622 and Shewana3_1623 are separated by 166 nucleotides
Shewana3_1620: Shewana3_1620 - ABC transporter related (RefSeq), at 1,907,308 to 1,908,216
_1620
Shewana3_1621: Shewana3_1621 - hypothetical protein (RefSeq), at 1,908,213 to 1,909,043
_1621
Shewana3_1622: Shewana3_1622 - hypothetical protein (RefSeq), at 1,909,131 to 1,909,787
_1622
Shewana3_1623: Shewana3_1623 - hypothetical protein (RefSeq), at 1,909,954 to 1,910,793
_1623
Position (kb)
1909
1910 Strain fitness (log2 ratio)
-2
-1
0
1 at 1908.200 kb on - strand at 1908.321 kb on - strand, within Shewana3_1621 at 1908.340 kb on - strand, within Shewana3_1621 at 1908.340 kb on - strand, within Shewana3_1621 at 1908.584 kb on + strand, within Shewana3_1621 at 1908.692 kb on + strand, within Shewana3_1621 at 1908.909 kb on + strand, within Shewana3_1621 at 1909.142 kb on - strand at 1909.309 kb on - strand, within Shewana3_1622 at 1909.309 kb on - strand, within Shewana3_1622 at 1909.314 kb on - strand, within Shewana3_1622 at 1909.632 kb on - strand, within Shewana3_1622 at 1909.689 kb on + strand, within Shewana3_1622 at 1909.697 kb on - strand, within Shewana3_1622 at 1909.726 kb on - strand at 1909.805 kb on + strand at 1910.182 kb on + strand, within Shewana3_1623 at 1910.368 kb on - strand, within Shewana3_1623 at 1910.368 kb on - strand, within Shewana3_1623 at 1910.485 kb on - strand, within Shewana3_1623 at 1910.736 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 1,908,200 - -0.5 1,908,321 - Shewana3_1621 0.13 +0.7 1,908,340 - Shewana3_1621 0.15 -0.9 1,908,340 - Shewana3_1621 0.15 +1.2 1,908,584 + Shewana3_1621 0.45 -0.6 1,908,692 + Shewana3_1621 0.58 -0.2 1,908,909 + Shewana3_1621 0.84 +1.2 1,909,142 - +1.1 1,909,309 - Shewana3_1622 0.27 +0.5 1,909,309 - Shewana3_1622 0.27 +0.6 1,909,314 - Shewana3_1622 0.28 +0.7 1,909,632 - Shewana3_1622 0.76 -0.5 1,909,689 + Shewana3_1622 0.85 -0.7 1,909,697 - Shewana3_1622 0.86 +0.2 1,909,726 - +1.2 1,909,805 + -0.6 1,910,182 + Shewana3_1623 0.27 -0.8 1,910,368 - Shewana3_1623 0.49 -2.0 1,910,368 - Shewana3_1623 0.49 +1.7 1,910,485 - Shewana3_1623 0.63 -0.0 1,910,736 + -1.3
Or see this region's nucleotide sequence