Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1541 and Shewana3_1542 are separated by 565 nucleotides Shewana3_1542 and folX are separated by 73 nucleotides folX and Shewana3_1544 are separated by 97 nucleotides
Shewana3_1541: Shewana3_1541 - sodium/glutamate symporter (RefSeq), at 1,798,073 to 1,799,296
_1541
Shewana3_1542: Shewana3_1542 - hypothetical protein (RefSeq), at 1,799,862 to 1,800,752
_1542
Shewana3_1543: folX - D-erythro-7,8-dihydroneopterin triphosphate 2'-epimerase (RefSeq), at 1,800,826 to 1,801,191
folX
Shewana3_1544: Shewana3_1544 - hypothetical protein (RefSeq), at 1,801,289 to 1,802,395
_1544
Position (kb)
1799
1800
1801 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1798.932 kb on - strand, within Shewana3_1541 at 1799.343 kb on - strand at 1799.369 kb on - strand at 1799.450 kb on - strand at 1799.460 kb on + strand at 1799.569 kb on - strand at 1799.571 kb on - strand at 1799.890 kb on + strand at 1799.949 kb on + strand at 1800.079 kb on + strand, within Shewana3_1542 at 1800.124 kb on - strand, within Shewana3_1542 at 1800.271 kb on - strand, within Shewana3_1542 at 1800.526 kb on + strand, within Shewana3_1542 at 1800.526 kb on + strand, within Shewana3_1542 at 1800.607 kb on - strand, within Shewana3_1542 at 1800.871 kb on + strand, within folX at 1801.395 kb on + strand at 1801.487 kb on - strand, within Shewana3_1544 at 1801.690 kb on + strand, within Shewana3_1544 at 1801.693 kb on - strand, within Shewana3_1544
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 1,798,932 - Shewana3_1541 0.70 -0.4 1,799,343 - +1.6 1,799,369 - +1.0 1,799,450 - +0.2 1,799,460 + -1.1 1,799,569 - -2.6 1,799,571 - -1.6 1,799,890 + -1.4 1,799,949 + -1.2 1,800,079 + Shewana3_1542 0.24 -0.6 1,800,124 - Shewana3_1542 0.29 -1.7 1,800,271 - Shewana3_1542 0.46 -0.8 1,800,526 + Shewana3_1542 0.75 -1.1 1,800,526 + Shewana3_1542 0.75 -0.6 1,800,607 - Shewana3_1542 0.84 -0.9 1,800,871 + folX Shewana3_1543 0.12 +0.2 1,801,395 + +0.4 1,801,487 - Shewana3_1544 0.18 +2.2 1,801,690 + Shewana3_1544 0.36 +0.3 1,801,693 - Shewana3_1544 0.36 +0.7
Or see this region's nucleotide sequence