Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1521 and Shewana3_1522 overlap by 4 nucleotides Shewana3_1522 and Shewana3_1523 are separated by 189 nucleotides Shewana3_1523 and Shewana3_1524 are separated by 3 nucleotides Shewana3_1524 and Shewana3_1525 overlap by 8 nucleotides
Shewana3_1521: Shewana3_1521 - phosphotransferase domain-containing protein (RefSeq), at 1,772,380 to 1,773,240
_1521
Shewana3_1522: Shewana3_1522 - translation factor SUA5 (RefSeq), at 1,773,237 to 1,773,857
_1522
Shewana3_1523: Shewana3_1523 - condensin subunit ScpA (RefSeq), at 1,774,047 to 1,774,835
_1523
Shewana3_1524: Shewana3_1524 - condensin subunit ScpB (RefSeq), at 1,774,839 to 1,775,435
_1524
Shewana3_1525: Shewana3_1525 - ribosomal large subunit pseudouridine synthase B (RefSeq), at 1,775,428 to 1,776,303
_1525
Position (kb)
1774
1775 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1773.289 kb on + strand at 1773.558 kb on - strand, within Shewana3_1522 at 1773.647 kb on - strand, within Shewana3_1522 at 1773.732 kb on + strand, within Shewana3_1522 at 1773.816 kb on + strand at 1773.967 kb on + strand at 1773.982 kb on + strand at 1773.982 kb on + strand at 1774.003 kb on + strand at 1774.326 kb on - strand, within Shewana3_1523 at 1774.342 kb on - strand, within Shewana3_1523 at 1774.517 kb on + strand, within Shewana3_1523 at 1774.722 kb on + strand, within Shewana3_1523 at 1774.730 kb on - strand, within Shewana3_1523 at 1774.972 kb on - strand, within Shewana3_1524 at 1775.002 kb on - strand, within Shewana3_1524 at 1775.081 kb on + strand, within Shewana3_1524 at 1775.122 kb on + strand, within Shewana3_1524 at 1775.449 kb on + strand at 1775.674 kb on + strand, within Shewana3_1525 at 1775.742 kb on - strand, within Shewana3_1525 at 1775.759 kb on + strand, within Shewana3_1525
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 1,773,289 + -0.5 1,773,558 - Shewana3_1522 0.52 -1.3 1,773,647 - Shewana3_1522 0.66 -0.1 1,773,732 + Shewana3_1522 0.80 +1.8 1,773,816 + +1.7 1,773,967 + +0.6 1,773,982 + +0.4 1,773,982 + +0.3 1,774,003 + -0.2 1,774,326 - Shewana3_1523 0.35 +0.6 1,774,342 - Shewana3_1523 0.37 -2.4 1,774,517 + Shewana3_1523 0.60 +0.2 1,774,722 + Shewana3_1523 0.86 +0.1 1,774,730 - Shewana3_1523 0.87 -2.0 1,774,972 - Shewana3_1524 0.22 +1.2 1,775,002 - Shewana3_1524 0.27 +2.2 1,775,081 + Shewana3_1524 0.41 +0.4 1,775,122 + Shewana3_1524 0.47 -0.1 1,775,449 + +1.0 1,775,674 + Shewana3_1525 0.28 -0.4 1,775,742 - Shewana3_1525 0.36 -0.8 1,775,759 + Shewana3_1525 0.38 -0.3
Or see this region's nucleotide sequence