Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1521 and Shewana3_1522 overlap by 4 nucleotides Shewana3_1522 and Shewana3_1523 are separated by 189 nucleotides Shewana3_1523 and Shewana3_1524 are separated by 3 nucleotides
Shewana3_1521: Shewana3_1521 - phosphotransferase domain-containing protein (RefSeq), at 1,772,380 to 1,773,240
_1521
Shewana3_1522: Shewana3_1522 - translation factor SUA5 (RefSeq), at 1,773,237 to 1,773,857
_1522
Shewana3_1523: Shewana3_1523 - condensin subunit ScpA (RefSeq), at 1,774,047 to 1,774,835
_1523
Shewana3_1524: Shewana3_1524 - condensin subunit ScpB (RefSeq), at 1,774,839 to 1,775,435
_1524
Position (kb)
1773
1774 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1772.404 kb on + strand at 1772.412 kb on - strand at 1772.564 kb on - strand, within Shewana3_1521 at 1772.636 kb on + strand, within Shewana3_1521 at 1772.649 kb on - strand, within Shewana3_1521 at 1772.785 kb on + strand, within Shewana3_1521 at 1772.853 kb on + strand, within Shewana3_1521 at 1772.853 kb on + strand, within Shewana3_1521 at 1772.956 kb on + strand, within Shewana3_1521 at 1772.974 kb on + strand, within Shewana3_1521 at 1772.975 kb on + strand, within Shewana3_1521 at 1772.975 kb on + strand, within Shewana3_1521 at 1772.975 kb on + strand, within Shewana3_1521 at 1773.289 kb on + strand at 1773.558 kb on - strand, within Shewana3_1522 at 1773.647 kb on - strand, within Shewana3_1522 at 1773.732 kb on + strand, within Shewana3_1522 at 1773.816 kb on + strand at 1773.967 kb on + strand at 1773.982 kb on + strand at 1773.982 kb on + strand at 1774.003 kb on + strand at 1774.326 kb on - strand, within Shewana3_1523 at 1774.342 kb on - strand, within Shewana3_1523 at 1774.517 kb on + strand, within Shewana3_1523 at 1774.722 kb on + strand, within Shewana3_1523 at 1774.730 kb on - strand, within Shewana3_1523
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 1,772,404 + +0.4 1,772,412 - +0.2 1,772,564 - Shewana3_1521 0.21 -0.3 1,772,636 + Shewana3_1521 0.30 +1.1 1,772,649 - Shewana3_1521 0.31 +0.8 1,772,785 + Shewana3_1521 0.47 +1.5 1,772,853 + Shewana3_1521 0.55 +0.7 1,772,853 + Shewana3_1521 0.55 +1.5 1,772,956 + Shewana3_1521 0.67 +1.1 1,772,974 + Shewana3_1521 0.69 +1.8 1,772,975 + Shewana3_1521 0.69 -0.3 1,772,975 + Shewana3_1521 0.69 +4.5 1,772,975 + Shewana3_1521 0.69 +0.6 1,773,289 + -0.5 1,773,558 - Shewana3_1522 0.52 -1.3 1,773,647 - Shewana3_1522 0.66 -0.1 1,773,732 + Shewana3_1522 0.80 +1.8 1,773,816 + +1.7 1,773,967 + +0.6 1,773,982 + +0.4 1,773,982 + +0.3 1,774,003 + -0.2 1,774,326 - Shewana3_1523 0.35 +0.6 1,774,342 - Shewana3_1523 0.37 -2.4 1,774,517 + Shewana3_1523 0.60 +0.2 1,774,722 + Shewana3_1523 0.86 +0.1 1,774,730 - Shewana3_1523 0.87 -2.0
Or see this region's nucleotide sequence