Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1480 and Shewana3_1481 overlap by 8 nucleotides Shewana3_1481 and Shewana3_1482 are separated by 110 nucleotides Shewana3_1482 and Shewana3_1483 are separated by 180 nucleotides
Shewana3_1480: Shewana3_1480 - FolC bifunctional protein (RefSeq), at 1,723,007 to 1,724,305
_1480
Shewana3_1481: Shewana3_1481 - sporulation domain-containing protein (RefSeq), at 1,724,298 to 1,725,122
_1481
Shewana3_1482: Shewana3_1482 - colicin V production protein (RefSeq), at 1,725,233 to 1,725,721
_1482
Shewana3_1483: Shewana3_1483 - amidophosphoribosyltransferase (RefSeq), at 1,725,902 to 1,727,416
_1483
Position (kb)
1724
1725
1726 Strain fitness (log2 ratio)
-1
0
1
2 at 1724.555 kb on + strand, within Shewana3_1481 at 1724.716 kb on + strand, within Shewana3_1481 at 1725.210 kb on + strand at 1725.334 kb on - strand, within Shewana3_1482 at 1725.450 kb on + strand, within Shewana3_1482 at 1725.458 kb on - strand, within Shewana3_1482 at 1725.591 kb on - strand, within Shewana3_1482 at 1725.976 kb on + strand at 1725.976 kb on + strand at 1726.041 kb on - strand at 1726.051 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 1,724,555 + Shewana3_1481 0.31 +1.1 1,724,716 + Shewana3_1481 0.51 -1.7 1,725,210 + +1.1 1,725,334 - Shewana3_1482 0.21 -0.2 1,725,450 + Shewana3_1482 0.44 -1.3 1,725,458 - Shewana3_1482 0.46 +2.1 1,725,591 - Shewana3_1482 0.73 +1.1 1,725,976 + +0.4 1,725,976 + +0.3 1,726,041 - +1.1 1,726,051 + -1.2
Or see this region's nucleotide sequence