Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1280 and Shewana3_1281 overlap by 4 nucleotides Shewana3_1281 and Shewana3_1282 overlap by 4 nucleotides
Shewana3_1280: Shewana3_1280 - conjugal transfer coupling protein TraG (RefSeq), at 1,491,078 to 1,493,075
_1280
Shewana3_1281: Shewana3_1281 - CopG/DNA-binding domain-containing protein (RefSeq), at 1,493,072 to 1,493,536
_1281
Shewana3_1282: Shewana3_1282 - type II secretion system protein E (RefSeq), at 1,493,533 to 1,494,603
_1282
Position (kb)
1493
1494 Strain fitness (log2 ratio)
-1
0
1 at 1492.396 kb on + strand, within Shewana3_1280 at 1492.404 kb on - strand, within Shewana3_1280 at 1492.442 kb on + strand, within Shewana3_1280 at 1492.852 kb on - strand, within Shewana3_1280 at 1492.959 kb on - strand at 1493.016 kb on + strand at 1493.380 kb on + strand, within Shewana3_1281 at 1493.444 kb on - strand, within Shewana3_1281 at 1493.494 kb on - strand at 1493.532 kb on + strand at 1493.532 kb on + strand at 1493.532 kb on + strand at 1493.540 kb on - strand at 1493.658 kb on - strand, within Shewana3_1282 at 1493.681 kb on - strand, within Shewana3_1282 at 1493.740 kb on - strand, within Shewana3_1282 at 1493.790 kb on - strand, within Shewana3_1282 at 1493.927 kb on + strand, within Shewana3_1282 at 1493.941 kb on - strand, within Shewana3_1282 at 1494.051 kb on + strand, within Shewana3_1282 at 1494.423 kb on + strand, within Shewana3_1282 at 1494.481 kb on - strand, within Shewana3_1282
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 1,492,396 + Shewana3_1280 0.66 -0.7 1,492,404 - Shewana3_1280 0.66 -0.9 1,492,442 + Shewana3_1280 0.68 +0.3 1,492,852 - Shewana3_1280 0.89 +0.1 1,492,959 - -0.5 1,493,016 + +0.2 1,493,380 + Shewana3_1281 0.66 +0.7 1,493,444 - Shewana3_1281 0.80 +0.7 1,493,494 - +0.5 1,493,532 + +1.2 1,493,532 + +1.2 1,493,532 + -1.5 1,493,540 - -0.3 1,493,658 - Shewana3_1282 0.12 -0.4 1,493,681 - Shewana3_1282 0.14 +0.4 1,493,740 - Shewana3_1282 0.19 -1.0 1,493,790 - Shewana3_1282 0.24 -0.2 1,493,927 + Shewana3_1282 0.37 -0.2 1,493,941 - Shewana3_1282 0.38 +0.2 1,494,051 + Shewana3_1282 0.48 +1.2 1,494,423 + Shewana3_1282 0.83 -0.1 1,494,481 - Shewana3_1282 0.89 +0.0
Or see this region's nucleotide sequence