Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1014 and Shewana3_1015 are separated by 13 nucleotides Shewana3_1015 and Shewana3_1016 are separated by 29 nucleotides Shewana3_1016 and Shewana3_1017 are separated by 250 nucleotides
Shewana3_1014: Shewana3_1014 - hypothetical protein (RefSeq), at 1,186,375 to 1,186,908
_1014
Shewana3_1015: Shewana3_1015 - lysozyme (RefSeq), at 1,186,922 to 1,187,437
_1015
Shewana3_1016: Shewana3_1016 - hypothetical protein (RefSeq), at 1,187,467 to 1,188,285
_1016
Shewana3_1017: Shewana3_1017 - transcription elongation factor GreA (RefSeq), at 1,188,536 to 1,189,012
_1017
Position (kb)
1187
1188
1189 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1186.520 kb on - strand, within Shewana3_1014 at 1186.646 kb on + strand, within Shewana3_1014 at 1186.646 kb on + strand, within Shewana3_1014 at 1186.654 kb on - strand, within Shewana3_1014 at 1186.654 kb on - strand, within Shewana3_1014 at 1186.707 kb on + strand, within Shewana3_1014 at 1186.748 kb on + strand, within Shewana3_1014 at 1186.890 kb on + strand at 1187.111 kb on - strand, within Shewana3_1015 at 1187.289 kb on - strand, within Shewana3_1015 at 1187.438 kb on + strand at 1187.594 kb on + strand, within Shewana3_1016 at 1187.672 kb on - strand, within Shewana3_1016 at 1187.751 kb on - strand, within Shewana3_1016 at 1187.913 kb on + strand, within Shewana3_1016 at 1188.358 kb on + strand at 1188.358 kb on + strand at 1188.444 kb on + strand at 1188.478 kb on + strand at 1188.493 kb on - strand at 1188.655 kb on - strand, within Shewana3_1017 at 1188.686 kb on - strand, within Shewana3_1017
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 1,186,520 - Shewana3_1014 0.27 +0.2 1,186,646 + Shewana3_1014 0.51 -0.4 1,186,646 + Shewana3_1014 0.51 +0.8 1,186,654 - Shewana3_1014 0.52 -1.5 1,186,654 - Shewana3_1014 0.52 +2.3 1,186,707 + Shewana3_1014 0.62 +4.1 1,186,748 + Shewana3_1014 0.70 +1.1 1,186,890 + -0.2 1,187,111 - Shewana3_1015 0.37 -2.3 1,187,289 - Shewana3_1015 0.71 -0.7 1,187,438 + -0.4 1,187,594 + Shewana3_1016 0.16 -0.3 1,187,672 - Shewana3_1016 0.25 -1.3 1,187,751 - Shewana3_1016 0.35 +0.4 1,187,913 + Shewana3_1016 0.54 +2.0 1,188,358 + +0.5 1,188,358 + +0.3 1,188,444 + +0.1 1,188,478 + -0.5 1,188,493 - -1.3 1,188,655 - Shewana3_1017 0.25 +1.9 1,188,686 - Shewana3_1017 0.31 -1.5
Or see this region's nucleotide sequence