Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0844 and Shewana3_0845 are separated by 171 nucleotides Shewana3_0845 and Shewana3_0846 are separated by 192 nucleotides
Shewana3_0844: Shewana3_0844 - ErfK/YbiS/YcfS/YnhG family protein (RefSeq), at 977,741 to 978,655
_0844
Shewana3_0845: Shewana3_0845 - anaerobic nitric oxide reductase transcription regulator (RefSeq), at 978,827 to 980,383
_0845
Shewana3_0846: Shewana3_0846 - putative nitric oxide reductase (subunit B) transmembrane protein (RefSeq), at 980,576 to 982,861
_0846
Position (kb) 978
979
980
981 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 977.857 kb on - strand, within Shewana3_0844 at 977.889 kb on - strand, within Shewana3_0844 at 977.906 kb on + strand, within Shewana3_0844 at 978.013 kb on - strand, within Shewana3_0844 at 978.038 kb on + strand, within Shewana3_0844 at 978.079 kb on - strand, within Shewana3_0844 at 978.090 kb on + strand, within Shewana3_0844 at 978.142 kb on - strand, within Shewana3_0844 at 978.241 kb on - strand, within Shewana3_0844 at 978.459 kb on - strand, within Shewana3_0844 at 978.517 kb on - strand, within Shewana3_0844 at 978.529 kb on - strand, within Shewana3_0844 at 978.529 kb on - strand, within Shewana3_0844 at 978.531 kb on + strand, within Shewana3_0844 at 978.539 kb on - strand, within Shewana3_0844 at 978.539 kb on - strand, within Shewana3_0844 at 978.604 kb on + strand at 978.719 kb on - strand at 978.744 kb on - strand at 978.954 kb on + strand at 979.147 kb on - strand, within Shewana3_0845 at 979.172 kb on + strand, within Shewana3_0845 at 979.180 kb on - strand, within Shewana3_0845 at 979.295 kb on - strand, within Shewana3_0845 at 979.297 kb on + strand, within Shewana3_0845 at 979.649 kb on + strand, within Shewana3_0845 at 979.649 kb on + strand, within Shewana3_0845 at 979.657 kb on - strand, within Shewana3_0845 at 979.907 kb on + strand, within Shewana3_0845 at 979.915 kb on - strand, within Shewana3_0845 at 979.938 kb on - strand, within Shewana3_0845 at 979.938 kb on - strand, within Shewana3_0845 at 979.938 kb on - strand, within Shewana3_0845 at 979.938 kb on - strand, within Shewana3_0845 at 979.985 kb on - strand, within Shewana3_0845 at 980.004 kb on + strand, within Shewana3_0845 at 980.199 kb on + strand, within Shewana3_0845 at 980.227 kb on + strand, within Shewana3_0845 at 980.242 kb on + strand at 980.273 kb on + strand at 980.454 kb on + strand at 980.476 kb on + strand at 980.512 kb on + strand at 980.535 kb on + strand at 980.626 kb on + strand at 980.660 kb on + strand at 980.660 kb on + strand at 980.699 kb on - strand at 980.751 kb on - strand at 980.819 kb on + strand, within Shewana3_0846 at 980.885 kb on + strand, within Shewana3_0846 at 980.931 kb on + strand, within Shewana3_0846 at 980.931 kb on - strand, within Shewana3_0846 at 980.946 kb on + strand, within Shewana3_0846 at 980.990 kb on + strand, within Shewana3_0846 at 980.990 kb on + strand, within Shewana3_0846 at 981.261 kb on - strand, within Shewana3_0846 at 981.292 kb on - strand, within Shewana3_0846
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 977,857 - Shewana3_0844 0.13 +1.3 977,889 - Shewana3_0844 0.16 +0.9 977,906 + Shewana3_0844 0.18 -1.2 978,013 - Shewana3_0844 0.30 +1.9 978,038 + Shewana3_0844 0.32 -0.5 978,079 - Shewana3_0844 0.37 +1.2 978,090 + Shewana3_0844 0.38 +1.4 978,142 - Shewana3_0844 0.44 +1.4 978,241 - Shewana3_0844 0.55 +1.6 978,459 - Shewana3_0844 0.78 +0.8 978,517 - Shewana3_0844 0.85 -0.0 978,529 - Shewana3_0844 0.86 +0.7 978,529 - Shewana3_0844 0.86 +0.6 978,531 + Shewana3_0844 0.86 -0.7 978,539 - Shewana3_0844 0.87 -0.1 978,539 - Shewana3_0844 0.87 -1.4 978,604 + +0.9 978,719 - -0.5 978,744 - -0.0 978,954 + -0.3 979,147 - Shewana3_0845 0.21 -1.5 979,172 + Shewana3_0845 0.22 -0.7 979,180 - Shewana3_0845 0.23 -0.2 979,295 - Shewana3_0845 0.30 -0.2 979,297 + Shewana3_0845 0.30 -0.2 979,649 + Shewana3_0845 0.53 +0.9 979,649 + Shewana3_0845 0.53 +0.1 979,657 - Shewana3_0845 0.53 -0.4 979,907 + Shewana3_0845 0.69 -2.3 979,915 - Shewana3_0845 0.70 +2.9 979,938 - Shewana3_0845 0.71 -2.5 979,938 - Shewana3_0845 0.71 -0.8 979,938 - Shewana3_0845 0.71 -3.1 979,938 - Shewana3_0845 0.71 -0.2 979,985 - Shewana3_0845 0.74 -0.1 980,004 + Shewana3_0845 0.76 +0.2 980,199 + Shewana3_0845 0.88 -1.2 980,227 + Shewana3_0845 0.90 -0.7 980,242 + -0.6 980,273 + -0.3 980,454 + +0.4 980,476 + +0.5 980,512 + +0.9 980,535 + +1.0 980,626 + -1.5 980,660 + -0.7 980,660 + -0.7 980,699 - +0.8 980,751 - +0.1 980,819 + Shewana3_0846 0.11 -0.5 980,885 + Shewana3_0846 0.14 -0.0 980,931 + Shewana3_0846 0.16 +0.6 980,931 - Shewana3_0846 0.16 +0.7 980,946 + Shewana3_0846 0.16 +2.7 980,990 + Shewana3_0846 0.18 -1.8 980,990 + Shewana3_0846 0.18 -1.5 981,261 - Shewana3_0846 0.30 -0.4 981,292 - Shewana3_0846 0.31 -0.0
Or see this region's nucleotide sequence