Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0834 and Shewana3_0835 are separated by 130 nucleotides Shewana3_0835 and Shewana3_0836 are separated by 75 nucleotides Shewana3_0836 and Shewana3_0837 are separated by 88 nucleotides
Shewana3_0834: Shewana3_0834 - hypothetical protein (RefSeq), at 966,913 to 967,872
_0834
Shewana3_0835: Shewana3_0835 - LysR family transcriptional regulator (RefSeq), at 968,003 to 968,986
_0835
Shewana3_0836: Shewana3_0836 - lysine exporter protein LysE/YggA (RefSeq), at 969,062 to 969,685
_0836
Shewana3_0837: Shewana3_0837 - NAD-dependent epimerase/dehydratase (RefSeq), at 969,774 to 970,709
_0837
Position (kb) 968
969 Strain fitness (log2 ratio) -2
-1
0
1 at 967.064 kb on - strand, within Shewana3_0834 at 967.289 kb on + strand, within Shewana3_0834 at 967.297 kb on - strand, within Shewana3_0834 at 967.381 kb on + strand, within Shewana3_0834 at 967.389 kb on - strand, within Shewana3_0834 at 967.389 kb on - strand, within Shewana3_0834 at 967.393 kb on + strand, within Shewana3_0834 at 967.480 kb on - strand, within Shewana3_0834 at 967.634 kb on - strand, within Shewana3_0834 at 967.646 kb on + strand, within Shewana3_0834 at 967.646 kb on + strand, within Shewana3_0834 at 967.646 kb on + strand, within Shewana3_0834 at 967.654 kb on - strand, within Shewana3_0834 at 967.749 kb on + strand, within Shewana3_0834 at 967.757 kb on - strand, within Shewana3_0834 at 967.781 kb on + strand at 968.067 kb on + strand at 968.196 kb on - strand, within Shewana3_0835 at 968.237 kb on - strand, within Shewana3_0835 at 968.258 kb on + strand, within Shewana3_0835 at 968.313 kb on + strand, within Shewana3_0835 at 968.401 kb on + strand, within Shewana3_0835 at 968.409 kb on - strand, within Shewana3_0835 at 968.578 kb on - strand, within Shewana3_0835 at 968.744 kb on + strand, within Shewana3_0835 at 968.752 kb on - strand, within Shewana3_0835 at 968.867 kb on + strand, within Shewana3_0835 at 968.986 kb on + strand at 969.010 kb on + strand at 969.018 kb on - strand at 969.172 kb on - strand, within Shewana3_0836 at 969.188 kb on + strand, within Shewana3_0836 at 969.230 kb on - strand, within Shewana3_0836 at 969.266 kb on + strand, within Shewana3_0836 at 969.266 kb on + strand, within Shewana3_0836 at 969.279 kb on + strand, within Shewana3_0836 at 969.355 kb on + strand, within Shewana3_0836 at 969.441 kb on - strand, within Shewana3_0836 at 969.521 kb on + strand, within Shewana3_0836 at 969.943 kb on + strand, within Shewana3_0837 at 969.968 kb on + strand, within Shewana3_0837
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 967,064 - Shewana3_0834 0.16 +0.3 967,289 + Shewana3_0834 0.39 -1.3 967,297 - Shewana3_0834 0.40 +0.3 967,381 + Shewana3_0834 0.49 -0.5 967,389 - Shewana3_0834 0.50 +0.1 967,389 - Shewana3_0834 0.50 +0.4 967,393 + Shewana3_0834 0.50 +0.2 967,480 - Shewana3_0834 0.59 +0.3 967,634 - Shewana3_0834 0.75 -0.3 967,646 + Shewana3_0834 0.76 -0.5 967,646 + Shewana3_0834 0.76 -0.4 967,646 + Shewana3_0834 0.76 +0.3 967,654 - Shewana3_0834 0.77 +0.0 967,749 + Shewana3_0834 0.87 +0.9 967,757 - Shewana3_0834 0.88 -0.4 967,781 + +1.2 968,067 + +0.2 968,196 - Shewana3_0835 0.20 -2.5 968,237 - Shewana3_0835 0.24 -0.8 968,258 + Shewana3_0835 0.26 -1.3 968,313 + Shewana3_0835 0.32 -0.3 968,401 + Shewana3_0835 0.40 +0.0 968,409 - Shewana3_0835 0.41 -0.8 968,578 - Shewana3_0835 0.58 -2.6 968,744 + Shewana3_0835 0.75 +0.5 968,752 - Shewana3_0835 0.76 +0.0 968,867 + Shewana3_0835 0.88 +0.3 968,986 + +1.0 969,010 + +0.8 969,018 - -2.7 969,172 - Shewana3_0836 0.18 -0.7 969,188 + Shewana3_0836 0.20 -0.1 969,230 - Shewana3_0836 0.27 -1.7 969,266 + Shewana3_0836 0.33 +0.2 969,266 + Shewana3_0836 0.33 +0.0 969,279 + Shewana3_0836 0.35 +0.2 969,355 + Shewana3_0836 0.47 +0.1 969,441 - Shewana3_0836 0.61 +0.2 969,521 + Shewana3_0836 0.74 +0.7 969,943 + Shewana3_0837 0.18 +0.5 969,968 + Shewana3_0837 0.21 -0.1
Or see this region's nucleotide sequence