Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0829 and Shewana3_0830 are separated by 4 nucleotides Shewana3_0830 and Shewana3_0831 are separated by 15 nucleotides
Shewana3_0829: Shewana3_0829 - spermidine synthase (RefSeq), at 958,991 to 960,724
_0829
Shewana3_0830: Shewana3_0830 - hypothetical protein (RefSeq), at 960,729 to 961,628
_0830
Shewana3_0831: Shewana3_0831 - hypothetical protein (RefSeq), at 961,644 to 962,621
_0831
Position (kb) 960
961
962 Strain fitness (log2 ratio) -2
-1
0
1 at 959.743 kb on + strand, within Shewana3_0829 at 960.072 kb on - strand, within Shewana3_0829 at 960.112 kb on + strand, within Shewana3_0829 at 960.112 kb on + strand, within Shewana3_0829 at 960.112 kb on + strand, within Shewana3_0829 at 960.112 kb on + strand, within Shewana3_0829 at 960.112 kb on + strand, within Shewana3_0829 at 960.120 kb on - strand, within Shewana3_0829 at 960.180 kb on + strand, within Shewana3_0829 at 960.202 kb on - strand, within Shewana3_0829 at 960.202 kb on - strand, within Shewana3_0829 at 960.213 kb on + strand, within Shewana3_0829 at 960.421 kb on + strand, within Shewana3_0829 at 960.587 kb on - strand at 960.685 kb on - strand at 960.726 kb on + strand at 960.943 kb on + strand, within Shewana3_0830 at 961.188 kb on - strand, within Shewana3_0830 at 961.193 kb on - strand, within Shewana3_0830 at 961.332 kb on - strand, within Shewana3_0830 at 961.399 kb on + strand, within Shewana3_0830 at 961.402 kb on + strand, within Shewana3_0830 at 961.414 kb on + strand, within Shewana3_0830 at 961.424 kb on - strand, within Shewana3_0830 at 961.424 kb on - strand, within Shewana3_0830 at 961.498 kb on - strand, within Shewana3_0830 at 961.498 kb on - strand, within Shewana3_0830 at 961.775 kb on - strand, within Shewana3_0831 at 962.346 kb on + strand, within Shewana3_0831 at 962.369 kb on - strand, within Shewana3_0831 at 962.427 kb on + strand, within Shewana3_0831 at 962.439 kb on - strand, within Shewana3_0831 at 962.449 kb on + strand, within Shewana3_0831 at 962.467 kb on + strand, within Shewana3_0831 at 962.509 kb on - strand, within Shewana3_0831 at 962.539 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 959,743 + Shewana3_0829 0.43 +1.5 960,072 - Shewana3_0829 0.62 +0.3 960,112 + Shewana3_0829 0.65 +1.6 960,112 + Shewana3_0829 0.65 -0.4 960,112 + Shewana3_0829 0.65 -0.2 960,112 + Shewana3_0829 0.65 -1.7 960,112 + Shewana3_0829 0.65 -0.3 960,120 - Shewana3_0829 0.65 -0.2 960,180 + Shewana3_0829 0.69 +0.6 960,202 - Shewana3_0829 0.70 +0.1 960,202 - Shewana3_0829 0.70 -1.6 960,213 + Shewana3_0829 0.70 -1.0 960,421 + Shewana3_0829 0.82 +0.4 960,587 - +0.4 960,685 - +1.0 960,726 + -1.0 960,943 + Shewana3_0830 0.24 -1.7 961,188 - Shewana3_0830 0.51 -0.0 961,193 - Shewana3_0830 0.52 -0.8 961,332 - Shewana3_0830 0.67 -1.2 961,399 + Shewana3_0830 0.74 -0.5 961,402 + Shewana3_0830 0.75 -0.4 961,414 + Shewana3_0830 0.76 -0.3 961,424 - Shewana3_0830 0.77 +0.5 961,424 - Shewana3_0830 0.77 +0.3 961,498 - Shewana3_0830 0.85 -0.3 961,498 - Shewana3_0830 0.85 +0.0 961,775 - Shewana3_0831 0.13 -2.1 962,346 + Shewana3_0831 0.72 -2.3 962,369 - Shewana3_0831 0.74 -0.4 962,427 + Shewana3_0831 0.80 +0.0 962,439 - Shewana3_0831 0.81 -2.1 962,449 + Shewana3_0831 0.82 -2.6 962,467 + Shewana3_0831 0.84 -0.7 962,509 - Shewana3_0831 0.88 -2.4 962,539 - -0.4
Or see this region's nucleotide sequence