Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0801 and Shewana3_0802 are separated by 25 nucleotides Shewana3_0802 and Shewana3_0803 are separated by 6 nucleotides Shewana3_0803 and Shewana3_0804 are separated by 145 nucleotides Shewana3_0804 and Shewana3_0805 are separated by 24 nucleotides
Shewana3_0801: Shewana3_0801 - hypothetical protein (RefSeq), at 924,849 to 925,223
_0801
Shewana3_0802: Shewana3_0802 - abortive infection protein (RefSeq), at 925,249 to 926,001
_0802
Shewana3_0803: Shewana3_0803 - MORN repeat-containing protein (RefSeq), at 926,008 to 928,281
_0803
Shewana3_0804: Shewana3_0804 - cupin 2 domain-containing protein (RefSeq), at 928,427 to 928,750
_0804
Shewana3_0805: Shewana3_0805 - hypothetical protein (RefSeq), at 928,775 to 929,947
_0805
Position (kb) 926
927
928
929 Strain fitness (log2 ratio) -2
-1
0
1
2 at 925.394 kb on + strand, within Shewana3_0802 at 925.402 kb on - strand, within Shewana3_0802 at 925.402 kb on - strand, within Shewana3_0802 at 925.402 kb on - strand, within Shewana3_0802 at 925.402 kb on - strand, within Shewana3_0802 at 925.404 kb on - strand, within Shewana3_0802 at 925.404 kb on - strand, within Shewana3_0802 at 925.404 kb on - strand, within Shewana3_0802 at 925.515 kb on - strand, within Shewana3_0802 at 925.555 kb on + strand, within Shewana3_0802 at 925.563 kb on - strand, within Shewana3_0802 at 925.638 kb on - strand, within Shewana3_0802 at 925.689 kb on - strand, within Shewana3_0802 at 925.714 kb on - strand, within Shewana3_0802 at 925.850 kb on + strand, within Shewana3_0802 at 925.850 kb on + strand, within Shewana3_0802 at 925.861 kb on + strand, within Shewana3_0802 at 925.906 kb on + strand, within Shewana3_0802 at 925.939 kb on + strand at 925.939 kb on + strand at 925.983 kb on - strand at 926.032 kb on - strand at 926.064 kb on + strand at 926.201 kb on + strand at 926.357 kb on - strand, within Shewana3_0803 at 926.536 kb on + strand, within Shewana3_0803 at 926.611 kb on - strand, within Shewana3_0803 at 926.737 kb on - strand, within Shewana3_0803 at 926.855 kb on + strand, within Shewana3_0803 at 927.247 kb on - strand, within Shewana3_0803 at 927.633 kb on + strand, within Shewana3_0803 at 927.714 kb on - strand, within Shewana3_0803 at 927.814 kb on + strand, within Shewana3_0803 at 927.822 kb on - strand, within Shewana3_0803 at 927.831 kb on - strand, within Shewana3_0803 at 927.903 kb on - strand, within Shewana3_0803 at 927.903 kb on - strand, within Shewana3_0803 at 928.019 kb on + strand, within Shewana3_0803 at 928.019 kb on + strand, within Shewana3_0803 at 928.027 kb on - strand, within Shewana3_0803 at 928.027 kb on - strand, within Shewana3_0803 at 928.095 kb on - strand at 928.177 kb on - strand at 928.198 kb on + strand at 928.208 kb on + strand at 928.504 kb on + strand, within Shewana3_0804 at 928.512 kb on - strand, within Shewana3_0804 at 928.646 kb on - strand, within Shewana3_0804 at 928.646 kb on - strand, within Shewana3_0804 at 928.670 kb on - strand, within Shewana3_0804 at 928.694 kb on - strand, within Shewana3_0804 at 928.716 kb on - strand, within Shewana3_0804 at 928.920 kb on + strand, within Shewana3_0805 at 929.029 kb on + strand, within Shewana3_0805 at 929.066 kb on + strand, within Shewana3_0805
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 925,394 + Shewana3_0802 0.19 +0.1 925,402 - Shewana3_0802 0.20 -1.8 925,402 - Shewana3_0802 0.20 +0.5 925,402 - Shewana3_0802 0.20 -2.1 925,402 - Shewana3_0802 0.20 -0.9 925,404 - Shewana3_0802 0.21 -0.5 925,404 - Shewana3_0802 0.21 +0.4 925,404 - Shewana3_0802 0.21 +0.1 925,515 - Shewana3_0802 0.35 -1.4 925,555 + Shewana3_0802 0.41 -1.1 925,563 - Shewana3_0802 0.42 -0.3 925,638 - Shewana3_0802 0.52 -0.5 925,689 - Shewana3_0802 0.58 -1.8 925,714 - Shewana3_0802 0.62 -1.0 925,850 + Shewana3_0802 0.80 -2.0 925,850 + Shewana3_0802 0.80 -2.3 925,861 + Shewana3_0802 0.81 +0.1 925,906 + Shewana3_0802 0.87 +0.9 925,939 + -0.3 925,939 + +0.4 925,983 - -0.5 926,032 - -1.3 926,064 + +0.7 926,201 + -0.2 926,357 - Shewana3_0803 0.15 -1.3 926,536 + Shewana3_0803 0.23 -1.0 926,611 - Shewana3_0803 0.27 -0.6 926,737 - Shewana3_0803 0.32 -2.0 926,855 + Shewana3_0803 0.37 -1.3 927,247 - Shewana3_0803 0.54 -0.2 927,633 + Shewana3_0803 0.71 -1.5 927,714 - Shewana3_0803 0.75 +0.2 927,814 + Shewana3_0803 0.79 +0.1 927,822 - Shewana3_0803 0.80 -0.4 927,831 - Shewana3_0803 0.80 -1.1 927,903 - Shewana3_0803 0.83 +1.0 927,903 - Shewana3_0803 0.83 +0.2 928,019 + Shewana3_0803 0.88 -0.0 928,019 + Shewana3_0803 0.88 +0.3 928,027 - Shewana3_0803 0.89 -1.5 928,027 - Shewana3_0803 0.89 -1.5 928,095 - -0.8 928,177 - -0.7 928,198 + -1.5 928,208 + +1.2 928,504 + Shewana3_0804 0.24 -0.3 928,512 - Shewana3_0804 0.26 +1.0 928,646 - Shewana3_0804 0.68 +1.9 928,646 - Shewana3_0804 0.68 -0.8 928,670 - Shewana3_0804 0.75 -0.5 928,694 - Shewana3_0804 0.82 -1.1 928,716 - Shewana3_0804 0.89 -1.6 928,920 + Shewana3_0805 0.12 +0.0 929,029 + Shewana3_0805 0.22 +0.9 929,066 + Shewana3_0805 0.25 +0.4
Or see this region's nucleotide sequence