Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0728 and Shewana3_0729 are separated by 26 nucleotides Shewana3_0729 and Shewana3_0730 are separated by 56 nucleotides Shewana3_0730 and Shewana3_0731 are separated by 52 nucleotides
Shewana3_0728: Shewana3_0728 - calcineurin phosphoesterase C-terminal domain-containing protein (RefSeq), at 844,152 to 844,991
_0728
Shewana3_0729: Shewana3_0729 - hypothetical protein (RefSeq), at 845,018 to 845,593
_0729
Shewana3_0730: Shewana3_0730 - DNA topoisomerase IV subunit B (RefSeq), at 845,650 to 847,536
_0730
Shewana3_0731: Shewana3_0731 - L-sorbosone dehydrogenase, putative (RefSeq), at 847,589 to 848,764
_0731
Position (kb) 845
846
847
848 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 844.685 kb on - strand, within Shewana3_0728 at 844.744 kb on - strand, within Shewana3_0728 at 844.749 kb on + strand, within Shewana3_0728 at 844.751 kb on - strand, within Shewana3_0728 at 844.756 kb on - strand, within Shewana3_0728 at 844.927 kb on + strand at 845.023 kb on + strand at 845.083 kb on + strand, within Shewana3_0729 at 845.091 kb on - strand, within Shewana3_0729 at 845.091 kb on - strand, within Shewana3_0729 at 845.276 kb on + strand, within Shewana3_0729 at 845.301 kb on + strand, within Shewana3_0729 at 845.320 kb on + strand, within Shewana3_0729 at 845.341 kb on + strand, within Shewana3_0729 at 845.470 kb on - strand, within Shewana3_0729 at 845.490 kb on + strand, within Shewana3_0729 at 847.530 kb on + strand at 847.531 kb on + strand at 847.974 kb on + strand, within Shewana3_0731 at 848.359 kb on + strand, within Shewana3_0731
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 844,685 - Shewana3_0728 0.63 +1.8 844,744 - Shewana3_0728 0.70 +1.8 844,749 + Shewana3_0728 0.71 +3.3 844,751 - Shewana3_0728 0.71 +2.4 844,756 - Shewana3_0728 0.72 +1.6 844,927 + +3.2 845,023 + +0.7 845,083 + Shewana3_0729 0.11 -0.4 845,091 - Shewana3_0729 0.13 -0.0 845,091 - Shewana3_0729 0.13 -1.3 845,276 + Shewana3_0729 0.45 +0.0 845,301 + Shewana3_0729 0.49 -0.0 845,320 + Shewana3_0729 0.52 -0.0 845,341 + Shewana3_0729 0.56 -3.4 845,470 - Shewana3_0729 0.78 -0.2 845,490 + Shewana3_0729 0.82 -0.1 847,530 + +1.1 847,531 + -0.2 847,974 + Shewana3_0731 0.33 -3.0 848,359 + Shewana3_0731 0.65 -0.4
Or see this region's nucleotide sequence