Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolC and Shewana3_0726 are separated by 243 nucleotides Shewana3_0726 and Shewana3_0727 overlap by 8 nucleotides Shewana3_0727 and Shewana3_0728 are separated by 15 nucleotides Shewana3_0728 and Shewana3_0729 are separated by 26 nucleotides
Shewana3_0725: tolC - outer membrane channel protein (RefSeq), at 841,529 to 842,836
tolC
Shewana3_0726: Shewana3_0726 - nucleoside diphosphate pyrophosphatase (RefSeq), at 843,080 to 843,691
_0726
Shewana3_0727: Shewana3_0727 - hypothetical protein (RefSeq), at 843,684 to 844,136
_0727
Shewana3_0728: Shewana3_0728 - calcineurin phosphoesterase C-terminal domain-containing protein (RefSeq), at 844,152 to 844,991
_0728
Shewana3_0729: Shewana3_0729 - hypothetical protein (RefSeq), at 845,018 to 845,593
_0729
Position (kb) 843
844
845 Strain fitness (log2 ratio) -1
0
1
2
3 at 842.740 kb on - strand at 842.960 kb on - strand at 842.984 kb on + strand at 843.003 kb on + strand at 843.248 kb on + strand, within Shewana3_0726 at 843.401 kb on + strand, within Shewana3_0726 at 843.462 kb on + strand, within Shewana3_0726 at 843.470 kb on - strand, within Shewana3_0726 at 843.484 kb on + strand, within Shewana3_0726 at 843.501 kb on - strand, within Shewana3_0726 at 843.510 kb on + strand, within Shewana3_0726 at 843.572 kb on + strand, within Shewana3_0726 at 843.580 kb on - strand, within Shewana3_0726 at 843.761 kb on + strand, within Shewana3_0727 at 843.791 kb on - strand, within Shewana3_0727 at 843.805 kb on + strand, within Shewana3_0727 at 843.821 kb on + strand, within Shewana3_0727 at 843.830 kb on + strand, within Shewana3_0727 at 843.937 kb on + strand, within Shewana3_0727 at 844.020 kb on + strand, within Shewana3_0727 at 844.020 kb on + strand, within Shewana3_0727 at 844.020 kb on + strand, within Shewana3_0727 at 844.020 kb on + strand, within Shewana3_0727 at 844.020 kb on + strand, within Shewana3_0727 at 844.028 kb on - strand, within Shewana3_0727 at 844.028 kb on - strand, within Shewana3_0727 at 844.028 kb on - strand, within Shewana3_0727 at 844.028 kb on - strand, within Shewana3_0727 at 844.028 kb on - strand, within Shewana3_0727 at 844.028 kb on - strand, within Shewana3_0727 at 844.028 kb on - strand, within Shewana3_0727 at 844.028 kb on - strand, within Shewana3_0727 at 844.078 kb on - strand, within Shewana3_0727 at 844.532 kb on + strand, within Shewana3_0728 at 844.598 kb on + strand, within Shewana3_0728 at 844.598 kb on + strand, within Shewana3_0728 at 844.606 kb on - strand, within Shewana3_0728 at 844.606 kb on - strand, within Shewana3_0728 at 844.648 kb on + strand, within Shewana3_0728 at 844.685 kb on - strand, within Shewana3_0728 at 844.744 kb on - strand, within Shewana3_0728 at 844.749 kb on + strand, within Shewana3_0728 at 844.751 kb on - strand, within Shewana3_0728 at 844.756 kb on - strand, within Shewana3_0728 at 844.927 kb on + strand at 845.023 kb on + strand at 845.083 kb on + strand, within Shewana3_0729 at 845.091 kb on - strand, within Shewana3_0729 at 845.091 kb on - strand, within Shewana3_0729
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 842,740 - -0.6 842,960 - -0.6 842,984 + -1.2 843,003 + -0.9 843,248 + Shewana3_0726 0.27 +0.1 843,401 + Shewana3_0726 0.52 +1.1 843,462 + Shewana3_0726 0.62 +1.0 843,470 - Shewana3_0726 0.64 +1.8 843,484 + Shewana3_0726 0.66 +0.2 843,501 - Shewana3_0726 0.69 -0.2 843,510 + Shewana3_0726 0.70 +0.5 843,572 + Shewana3_0726 0.80 +1.1 843,580 - Shewana3_0726 0.82 -0.1 843,761 + Shewana3_0727 0.17 +0.6 843,791 - Shewana3_0727 0.24 +0.4 843,805 + Shewana3_0727 0.27 +2.0 843,821 + Shewana3_0727 0.30 +1.7 843,830 + Shewana3_0727 0.32 +1.1 843,937 + Shewana3_0727 0.56 +1.2 844,020 + Shewana3_0727 0.74 +2.8 844,020 + Shewana3_0727 0.74 +2.0 844,020 + Shewana3_0727 0.74 -0.6 844,020 + Shewana3_0727 0.74 +1.2 844,020 + Shewana3_0727 0.74 +2.1 844,028 - Shewana3_0727 0.76 -0.0 844,028 - Shewana3_0727 0.76 -0.5 844,028 - Shewana3_0727 0.76 -1.5 844,028 - Shewana3_0727 0.76 -0.4 844,028 - Shewana3_0727 0.76 +1.2 844,028 - Shewana3_0727 0.76 +1.1 844,028 - Shewana3_0727 0.76 -0.3 844,028 - Shewana3_0727 0.76 +1.6 844,078 - Shewana3_0727 0.87 +0.6 844,532 + Shewana3_0728 0.45 +3.1 844,598 + Shewana3_0728 0.53 +3.1 844,598 + Shewana3_0728 0.53 +3.0 844,606 - Shewana3_0728 0.54 +1.2 844,606 - Shewana3_0728 0.54 +0.5 844,648 + Shewana3_0728 0.59 +2.8 844,685 - Shewana3_0728 0.63 +1.8 844,744 - Shewana3_0728 0.70 +1.8 844,749 + Shewana3_0728 0.71 +3.3 844,751 - Shewana3_0728 0.71 +2.4 844,756 - Shewana3_0728 0.72 +1.6 844,927 + +3.2 845,023 + +0.7 845,083 + Shewana3_0729 0.11 -0.4 845,091 - Shewana3_0729 0.13 -0.0 845,091 - Shewana3_0729 0.13 -1.3
Or see this region's nucleotide sequence