Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0702 and rpsR are separated by 11 nucleotides rpsR and rplI are separated by 36 nucleotides rplI and Shewana3_0705 are separated by 157 nucleotides Shewana3_0705 and Shewana3_0706 overlap by 17 nucleotides
Shewana3_0702: Shewana3_0702 - primosomal replication protein N (RefSeq), at 818,705 to 818,995
_0702
Shewana3_0703: rpsR - 30S ribosomal protein S18 (RefSeq), at 819,007 to 819,234
rpsR
Shewana3_0704: rplI - 50S ribosomal protein L9 (RefSeq), at 819,271 to 819,723
rplI
Shewana3_0705: Shewana3_0705 - small GTP-binding protein (RefSeq), at 819,881 to 821,119
_0705
Shewana3_0706: Shewana3_0706 - biotin synthase (RefSeq), at 821,103 to 822,182
_0706
Position (kb) 819
820
821
822 Strain fitness (log2 ratio) -1
0
1 at 819.451 kb on + strand, within rplI at 819.844 kb on + strand at 819.979 kb on - strand at 820.065 kb on - strand, within Shewana3_0705 at 820.271 kb on - strand, within Shewana3_0705 at 820.655 kb on + strand, within Shewana3_0705 at 820.686 kb on + strand, within Shewana3_0705 at 820.700 kb on + strand, within Shewana3_0705 at 820.785 kb on - strand, within Shewana3_0705 at 820.880 kb on + strand, within Shewana3_0705 at 821.011 kb on + strand at 821.109 kb on + strand at 821.144 kb on + strand at 821.144 kb on + strand at 821.144 kb on + strand at 821.170 kb on - strand at 821.402 kb on + strand, within Shewana3_0706 at 821.402 kb on + strand, within Shewana3_0706 at 821.584 kb on - strand, within Shewana3_0706 at 821.841 kb on + strand, within Shewana3_0706 at 821.854 kb on - strand, within Shewana3_0706 at 821.877 kb on - strand, within Shewana3_0706 at 822.083 kb on + strand at 822.102 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 819,451 + rplI Shewana3_0704 0.40 -1.7 819,844 + -1.0 819,979 - +1.4 820,065 - Shewana3_0705 0.15 -0.6 820,271 - Shewana3_0705 0.31 -0.8 820,655 + Shewana3_0705 0.62 -1.0 820,686 + Shewana3_0705 0.65 -1.2 820,700 + Shewana3_0705 0.66 -1.3 820,785 - Shewana3_0705 0.73 -0.3 820,880 + Shewana3_0705 0.81 -0.3 821,011 + -0.2 821,109 + -0.4 821,144 + +0.1 821,144 + -0.3 821,144 + +1.0 821,170 - -0.5 821,402 + Shewana3_0706 0.28 -0.3 821,402 + Shewana3_0706 0.28 -0.7 821,584 - Shewana3_0706 0.45 -0.7 821,841 + Shewana3_0706 0.68 -0.7 821,854 - Shewana3_0706 0.70 +1.2 821,877 - Shewana3_0706 0.72 -1.6 822,083 + -1.2 822,102 + -1.0
Or see this region's nucleotide sequence