Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rplM and rpsI are separated by 14 nucleotides rpsI and Shewana3_0694 are separated by 113 nucleotides Shewana3_0694 and Shewana3_0695 are separated by 66 nucleotides Shewana3_0695 and Shewana3_0696 are separated by 108 nucleotides Shewana3_0696 and Shewana3_0697 are separated by 39 nucleotides
Shewana3_0692: rplM - 50S ribosomal protein L13 (RefSeq), at 809,168 to 809,596
rplM
Shewana3_0693: rpsI - 30S ribosomal protein S9 (RefSeq), at 809,611 to 810,003
rpsI
Shewana3_0694: Shewana3_0694 - hypothetical protein (RefSeq), at 810,117 to 810,305
_0694
Shewana3_0695: Shewana3_0695 - adenylosuccinate synthetase (RefSeq), at 810,372 to 811,667
_0695
Shewana3_0696: Shewana3_0696 - Sel1 domain-containing protein (RefSeq), at 811,776 to 812,432
_0696
Shewana3_0697: Shewana3_0697 - RNAse R (RefSeq), at 812,472 to 814,901
_0697
Position (kb) 810
811
812 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 810.099 kb on + strand at 810.121 kb on + strand at 810.248 kb on - strand, within Shewana3_0694 at 810.279 kb on - strand, within Shewana3_0694 at 810.390 kb on + strand at 810.689 kb on + strand, within Shewana3_0695 at 810.706 kb on + strand, within Shewana3_0695 at 810.716 kb on - strand, within Shewana3_0695 at 810.788 kb on + strand, within Shewana3_0695 at 810.827 kb on + strand, within Shewana3_0695 at 811.278 kb on + strand, within Shewana3_0695 at 811.365 kb on + strand, within Shewana3_0695 at 811.465 kb on + strand, within Shewana3_0695 at 811.534 kb on + strand, within Shewana3_0695 at 811.628 kb on + strand at 811.958 kb on - strand, within Shewana3_0696 at 812.042 kb on + strand, within Shewana3_0696 at 812.051 kb on - strand, within Shewana3_0696 at 812.078 kb on - strand, within Shewana3_0696 at 812.262 kb on + strand, within Shewana3_0696 at 812.507 kb on - strand at 812.644 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 810,099 + -0.6 810,121 + -0.1 810,248 - Shewana3_0694 0.69 -0.5 810,279 - Shewana3_0694 0.86 -2.7 810,390 + -0.1 810,689 + Shewana3_0695 0.24 +0.9 810,706 + Shewana3_0695 0.26 +0.4 810,716 - Shewana3_0695 0.27 +1.9 810,788 + Shewana3_0695 0.32 +0.6 810,827 + Shewana3_0695 0.35 +1.9 811,278 + Shewana3_0695 0.70 +2.0 811,365 + Shewana3_0695 0.77 +1.9 811,465 + Shewana3_0695 0.84 +2.0 811,534 + Shewana3_0695 0.90 +1.9 811,628 + +0.7 811,958 - Shewana3_0696 0.28 -0.8 812,042 + Shewana3_0696 0.40 +2.3 812,051 - Shewana3_0696 0.42 -4.1 812,078 - Shewana3_0696 0.46 -2.2 812,262 + Shewana3_0696 0.74 +1.6 812,507 - -3.1 812,644 + -1.6
Or see this region's nucleotide sequence