Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0607 and Shewana3_0608 are separated by 199 nucleotides Shewana3_0608 and Shewana3_0609 are separated by 55 nucleotides
Shewana3_0607: Shewana3_0607 - peptidase S9 prolyl oligopeptidase (RefSeq), at 709,775 to 712,255
_0607
Shewana3_0608: Shewana3_0608 - putative signal transduction protein (RefSeq), at 712,455 to 713,600
_0608
Shewana3_0609: Shewana3_0609 - alpha/beta hydrolase domain-containing protein (RefSeq), at 713,656 to 714,567
_0609
Position (kb) 712
713
714 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 711.458 kb on - strand, within Shewana3_0607 at 711.687 kb on - strand, within Shewana3_0607 at 711.708 kb on - strand, within Shewana3_0607 at 711.719 kb on + strand, within Shewana3_0607 at 711.792 kb on + strand, within Shewana3_0607 at 711.959 kb on + strand, within Shewana3_0607 at 712.335 kb on + strand at 712.343 kb on - strand at 712.541 kb on + strand at 712.797 kb on - strand, within Shewana3_0608 at 712.838 kb on - strand, within Shewana3_0608 at 713.116 kb on - strand, within Shewana3_0608 at 713.124 kb on + strand, within Shewana3_0608 at 713.379 kb on - strand, within Shewana3_0608 at 713.431 kb on - strand, within Shewana3_0608 at 713.458 kb on - strand, within Shewana3_0608 at 713.561 kb on + strand at 713.594 kb on + strand at 713.842 kb on - strand, within Shewana3_0609 at 714.194 kb on - strand, within Shewana3_0609 at 714.287 kb on + strand, within Shewana3_0609
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 711,458 - Shewana3_0607 0.68 +0.2 711,687 - Shewana3_0607 0.77 -0.5 711,708 - Shewana3_0607 0.78 +0.3 711,719 + Shewana3_0607 0.78 +0.0 711,792 + Shewana3_0607 0.81 -0.3 711,959 + Shewana3_0607 0.88 +0.6 712,335 + -0.6 712,343 - +1.1 712,541 + -1.0 712,797 - Shewana3_0608 0.30 -2.0 712,838 - Shewana3_0608 0.33 -1.7 713,116 - Shewana3_0608 0.58 -1.1 713,124 + Shewana3_0608 0.58 +0.1 713,379 - Shewana3_0608 0.81 +0.4 713,431 - Shewana3_0608 0.85 -1.4 713,458 - Shewana3_0608 0.88 +0.1 713,561 + -0.5 713,594 + +0.8 713,842 - Shewana3_0609 0.20 -3.6 714,194 - Shewana3_0609 0.59 -0.8 714,287 + Shewana3_0609 0.69 +0.1
Or see this region's nucleotide sequence