Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0606 and Shewana3_0607 are separated by 455 nucleotides Shewana3_0607 and Shewana3_0608 are separated by 199 nucleotides
Shewana3_0606: Shewana3_0606 - aminodeoxychorismate synthase, glutamine amidotransferase subunit (RefSeq), at 708,744 to 709,319
_0606
Shewana3_0607: Shewana3_0607 - peptidase S9 prolyl oligopeptidase (RefSeq), at 709,775 to 712,255
_0607
Shewana3_0608: Shewana3_0608 - putative signal transduction protein (RefSeq), at 712,455 to 713,600
_0608
Position (kb) 709
710
711
712
713 Strain fitness (log2 ratio) -2
-1
0
1 at 708.810 kb on + strand, within Shewana3_0606 at 708.900 kb on - strand, within Shewana3_0606 at 709.085 kb on + strand, within Shewana3_0606 at 709.343 kb on + strand at 709.351 kb on - strand at 709.499 kb on + strand at 709.757 kb on + strand at 709.886 kb on + strand at 709.926 kb on + strand at 709.926 kb on + strand at 709.926 kb on + strand at 709.948 kb on + strand at 710.075 kb on - strand, within Shewana3_0607 at 710.135 kb on + strand, within Shewana3_0607 at 710.143 kb on - strand, within Shewana3_0607 at 710.153 kb on - strand, within Shewana3_0607 at 710.300 kb on - strand, within Shewana3_0607 at 710.410 kb on + strand, within Shewana3_0607 at 710.542 kb on + strand, within Shewana3_0607 at 710.769 kb on + strand, within Shewana3_0607 at 710.803 kb on + strand, within Shewana3_0607 at 710.824 kb on + strand, within Shewana3_0607 at 710.832 kb on - strand, within Shewana3_0607 at 711.099 kb on - strand, within Shewana3_0607 at 711.124 kb on + strand, within Shewana3_0607 at 711.233 kb on + strand, within Shewana3_0607 at 711.241 kb on - strand, within Shewana3_0607 at 711.417 kb on + strand, within Shewana3_0607 at 711.458 kb on - strand, within Shewana3_0607 at 711.687 kb on - strand, within Shewana3_0607 at 711.708 kb on - strand, within Shewana3_0607 at 711.719 kb on + strand, within Shewana3_0607 at 711.792 kb on + strand, within Shewana3_0607 at 711.959 kb on + strand, within Shewana3_0607 at 712.335 kb on + strand at 712.343 kb on - strand at 712.541 kb on + strand at 712.797 kb on - strand, within Shewana3_0608 at 712.838 kb on - strand, within Shewana3_0608 at 713.116 kb on - strand, within Shewana3_0608 at 713.124 kb on + strand, within Shewana3_0608
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 708,810 + Shewana3_0606 0.11 +0.8 708,900 - Shewana3_0606 0.27 +0.2 709,085 + Shewana3_0606 0.59 -0.0 709,343 + +0.3 709,351 - -0.8 709,499 + -0.6 709,757 + +0.8 709,886 + -0.4 709,926 + +1.2 709,926 + -0.5 709,926 + +0.8 709,948 + -1.8 710,075 - Shewana3_0607 0.12 +0.4 710,135 + Shewana3_0607 0.15 +0.1 710,143 - Shewana3_0607 0.15 +0.5 710,153 - Shewana3_0607 0.15 +0.1 710,300 - Shewana3_0607 0.21 -0.5 710,410 + Shewana3_0607 0.26 -2.2 710,542 + Shewana3_0607 0.31 -0.7 710,769 + Shewana3_0607 0.40 +0.0 710,803 + Shewana3_0607 0.41 -0.8 710,824 + Shewana3_0607 0.42 -0.3 710,832 - Shewana3_0607 0.43 +1.0 711,099 - Shewana3_0607 0.53 +1.1 711,124 + Shewana3_0607 0.54 -0.4 711,233 + Shewana3_0607 0.59 -0.3 711,241 - Shewana3_0607 0.59 +0.2 711,417 + Shewana3_0607 0.66 -1.4 711,458 - Shewana3_0607 0.68 +0.2 711,687 - Shewana3_0607 0.77 -0.5 711,708 - Shewana3_0607 0.78 +0.3 711,719 + Shewana3_0607 0.78 +0.0 711,792 + Shewana3_0607 0.81 -0.3 711,959 + Shewana3_0607 0.88 +0.6 712,335 + -0.6 712,343 - +1.1 712,541 + -1.0 712,797 - Shewana3_0608 0.30 -2.0 712,838 - Shewana3_0608 0.33 -1.7 713,116 - Shewana3_0608 0.58 -1.1 713,124 + Shewana3_0608 0.58 +0.1
Or see this region's nucleotide sequence