Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0554 and Shewana3_0555 are separated by 138 nucleotides Shewana3_0555 and Shewana3_0556 are separated by 48 nucleotides Shewana3_0556 and Shewana3_0557 are separated by 142 nucleotides
Shewana3_0554: Shewana3_0554 - diguanylate cyclase/phosphodiesterase (RefSeq), at 646,700 to 648,454
_0554
Shewana3_0555: Shewana3_0555 - hypothetical protein (RefSeq), at 648,593 to 648,901
_0555
Shewana3_0556: Shewana3_0556 - GumN family protein (RefSeq), at 648,950 to 649,846
_0556
Shewana3_0557: Shewana3_0557 - methyltransferase type 11 (RefSeq), at 649,989 to 650,774
_0557
Position (kb) 648
649
650 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 648.138 kb on - strand, within Shewana3_0554 at 648.156 kb on - strand, within Shewana3_0554 at 648.282 kb on + strand at 648.290 kb on - strand at 648.325 kb on - strand at 648.340 kb on + strand at 648.425 kb on - strand at 648.477 kb on - strand at 648.654 kb on - strand, within Shewana3_0555 at 648.678 kb on - strand, within Shewana3_0555 at 648.678 kb on - strand, within Shewana3_0555 at 648.761 kb on - strand, within Shewana3_0555 at 648.763 kb on + strand, within Shewana3_0555 at 648.893 kb on - strand at 648.893 kb on - strand at 648.893 kb on - strand at 648.893 kb on - strand at 648.938 kb on - strand at 649.015 kb on + strand at 649.053 kb on - strand, within Shewana3_0556 at 649.172 kb on - strand, within Shewana3_0556 at 649.308 kb on + strand, within Shewana3_0556 at 649.328 kb on + strand, within Shewana3_0556 at 649.423 kb on - strand, within Shewana3_0556 at 649.438 kb on + strand, within Shewana3_0556 at 649.481 kb on + strand, within Shewana3_0556 at 649.515 kb on + strand, within Shewana3_0556 at 649.567 kb on + strand, within Shewana3_0556 at 649.654 kb on + strand, within Shewana3_0556 at 649.841 kb on + strand at 649.920 kb on - strand at 650.075 kb on - strand, within Shewana3_0557 at 650.161 kb on - strand, within Shewana3_0557 at 650.263 kb on - strand, within Shewana3_0557 at 650.263 kb on - strand, within Shewana3_0557 at 650.355 kb on - strand, within Shewana3_0557 at 650.577 kb on + strand, within Shewana3_0557 at 650.585 kb on - strand, within Shewana3_0557 at 650.719 kb on + strand at 650.784 kb on - strand at 650.786 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 648,138 - Shewana3_0554 0.82 +0.2 648,156 - Shewana3_0554 0.83 -0.3 648,282 + +1.0 648,290 - -2.6 648,325 - -0.6 648,340 + +0.4 648,425 - -0.5 648,477 - -0.1 648,654 - Shewana3_0555 0.20 +0.7 648,678 - Shewana3_0555 0.28 -0.1 648,678 - Shewana3_0555 0.28 +0.4 648,761 - Shewana3_0555 0.54 +0.4 648,763 + Shewana3_0555 0.55 -0.1 648,893 - -0.4 648,893 - +0.5 648,893 - -0.5 648,893 - +0.7 648,938 - -0.1 649,015 + -0.1 649,053 - Shewana3_0556 0.11 -2.2 649,172 - Shewana3_0556 0.25 -1.6 649,308 + Shewana3_0556 0.40 -0.4 649,328 + Shewana3_0556 0.42 +0.3 649,423 - Shewana3_0556 0.53 -1.2 649,438 + Shewana3_0556 0.54 -0.3 649,481 + Shewana3_0556 0.59 +0.2 649,515 + Shewana3_0556 0.63 -0.5 649,567 + Shewana3_0556 0.69 +0.0 649,654 + Shewana3_0556 0.78 -0.2 649,841 + +0.3 649,920 - -0.8 650,075 - Shewana3_0557 0.11 +1.0 650,161 - Shewana3_0557 0.22 +0.2 650,263 - Shewana3_0557 0.35 +0.1 650,263 - Shewana3_0557 0.35 +0.3 650,355 - Shewana3_0557 0.47 +0.2 650,577 + Shewana3_0557 0.75 -1.9 650,585 - Shewana3_0557 0.76 +0.5 650,719 + -4.1 650,784 - +1.1 650,786 + -1.2
Or see this region's nucleotide sequence