Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0529 and Shewana3_0530 are separated by 234 nucleotides
Shewana3_0529: Shewana3_0529 - hypothetical protein (RefSeq), at 623,259 to 624,098
_0529
Shewana3_0530: Shewana3_0530 - LysR family transcriptional regulator (RefSeq), at 624,333 to 625,199
_0530
Position (kb) 623
624
625 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 622.630 kb on + strand at 622.631 kb on + strand at 622.631 kb on + strand at 622.631 kb on + strand at 622.631 kb on + strand at 622.638 kb on - strand at 622.639 kb on - strand at 622.639 kb on - strand at 622.639 kb on - strand at 622.639 kb on - strand at 622.658 kb on + strand at 622.666 kb on - strand at 622.666 kb on - strand at 622.668 kb on + strand at 622.682 kb on - strand at 622.687 kb on - strand at 622.693 kb on - strand at 622.699 kb on + strand at 622.700 kb on + strand at 622.700 kb on + strand at 622.723 kb on + strand at 622.745 kb on + strand at 622.745 kb on + strand at 622.745 kb on + strand at 622.745 kb on + strand at 622.745 kb on + strand at 622.745 kb on + strand at 622.745 kb on + strand at 622.753 kb on - strand at 622.753 kb on - strand at 622.753 kb on - strand at 622.753 kb on - strand at 622.753 kb on - strand at 622.753 kb on - strand at 622.753 kb on - strand at 622.805 kb on + strand at 622.822 kb on - strand at 622.822 kb on - strand at 622.832 kb on + strand at 622.838 kb on + strand at 622.838 kb on + strand at 622.838 kb on + strand at 622.838 kb on + strand at 622.838 kb on + strand at 622.838 kb on + strand at 622.838 kb on + strand at 622.838 kb on + strand at 622.838 kb on + strand at 622.838 kb on + strand at 622.840 kb on + strand at 622.840 kb on - strand at 622.846 kb on - strand at 622.846 kb on - strand at 622.846 kb on - strand at 622.846 kb on - strand at 622.846 kb on - strand at 622.846 kb on - strand at 622.846 kb on - strand at 622.846 kb on - strand at 622.848 kb on - strand at 622.848 kb on - strand at 622.850 kb on + strand at 622.858 kb on - strand at 622.907 kb on + strand at 622.913 kb on + strand at 622.955 kb on - strand at 622.964 kb on + strand at 622.974 kb on + strand at 622.974 kb on + strand at 622.980 kb on - strand at 622.996 kb on + strand at 622.998 kb on + strand at 623.004 kb on - strand at 623.004 kb on - strand at 623.004 kb on - strand at 623.030 kb on + strand at 623.065 kb on + strand at 623.068 kb on - strand at 623.073 kb on - strand at 623.082 kb on + strand at 623.082 kb on + strand at 623.082 kb on + strand at 623.084 kb on + strand at 623.084 kb on + strand at 623.084 kb on + strand at 623.084 kb on + strand at 623.090 kb on - strand at 623.104 kb on + strand at 623.108 kb on - strand at 623.124 kb on - strand at 623.142 kb on + strand at 623.176 kb on - strand at 623.188 kb on + strand at 623.188 kb on + strand at 623.205 kb on + strand at 623.205 kb on + strand at 623.205 kb on + strand at 623.213 kb on - strand at 623.213 kb on - strand at 623.215 kb on + strand at 623.276 kb on + strand at 623.276 kb on + strand at 623.276 kb on + strand at 623.282 kb on + strand at 623.284 kb on - strand at 623.284 kb on - strand at 623.284 kb on - strand at 623.284 kb on - strand at 623.285 kb on - strand at 623.285 kb on - strand at 623.285 kb on - strand at 623.285 kb on - strand at 623.285 kb on - strand at 623.295 kb on - strand at 623.322 kb on + strand at 623.322 kb on + strand at 623.362 kb on - strand, within Shewana3_0529 at 623.376 kb on + strand, within Shewana3_0529 at 623.376 kb on + strand, within Shewana3_0529 at 623.376 kb on + strand, within Shewana3_0529 at 623.376 kb on + strand, within Shewana3_0529 at 623.384 kb on - strand, within Shewana3_0529 at 623.384 kb on - strand, within Shewana3_0529 at 623.384 kb on - strand, within Shewana3_0529 at 623.386 kb on + strand, within Shewana3_0529 at 623.386 kb on + strand, within Shewana3_0529 at 623.386 kb on + strand, within Shewana3_0529 at 623.386 kb on + strand, within Shewana3_0529 at 623.386 kb on + strand, within Shewana3_0529 at 623.386 kb on + strand, within Shewana3_0529 at 623.386 kb on + strand, within Shewana3_0529 at 623.386 kb on + strand, within Shewana3_0529 at 623.386 kb on + strand, within Shewana3_0529 at 623.386 kb on + strand, within Shewana3_0529 at 623.394 kb on - strand, within Shewana3_0529 at 623.394 kb on - strand, within Shewana3_0529 at 623.394 kb on - strand, within Shewana3_0529 at 623.394 kb on - strand, within Shewana3_0529 at 623.394 kb on - strand, within Shewana3_0529 at 623.394 kb on - strand, within Shewana3_0529 at 623.397 kb on + strand, within Shewana3_0529 at 623.397 kb on + strand, within Shewana3_0529 at 623.397 kb on + strand, within Shewana3_0529 at 623.405 kb on - strand, within Shewana3_0529 at 623.421 kb on + strand, within Shewana3_0529 at 623.421 kb on + strand, within Shewana3_0529 at 623.421 kb on + strand, within Shewana3_0529 at 623.430 kb on + strand, within Shewana3_0529 at 623.433 kb on + strand, within Shewana3_0529 at 623.468 kb on + strand, within Shewana3_0529 at 623.468 kb on + strand, within Shewana3_0529 at 623.476 kb on - strand, within Shewana3_0529 at 623.476 kb on - strand, within Shewana3_0529 at 623.477 kb on + strand, within Shewana3_0529 at 623.488 kb on - strand, within Shewana3_0529 at 623.491 kb on + strand, within Shewana3_0529 at 623.491 kb on + strand, within Shewana3_0529 at 623.491 kb on + strand, within Shewana3_0529 at 623.499 kb on - strand, within Shewana3_0529 at 623.499 kb on - strand, within Shewana3_0529 at 623.499 kb on - strand, within Shewana3_0529 at 623.499 kb on - strand, within Shewana3_0529 at 623.501 kb on + strand, within Shewana3_0529 at 623.567 kb on + strand, within Shewana3_0529 at 623.567 kb on + strand, within Shewana3_0529 at 623.567 kb on + strand, within Shewana3_0529 at 623.598 kb on - strand, within Shewana3_0529 at 623.659 kb on + strand, within Shewana3_0529 at 623.667 kb on - strand, within Shewana3_0529 at 623.667 kb on - strand, within Shewana3_0529 at 623.669 kb on + strand, within Shewana3_0529 at 623.669 kb on + strand, within Shewana3_0529 at 623.669 kb on + strand, within Shewana3_0529 at 623.669 kb on + strand, within Shewana3_0529 at 623.671 kb on + strand, within Shewana3_0529 at 623.671 kb on + strand, within Shewana3_0529 at 623.671 kb on + strand, within Shewana3_0529 at 623.671 kb on + strand, within Shewana3_0529 at 623.671 kb on + strand, within Shewana3_0529 at 623.671 kb on + strand, within Shewana3_0529 at 623.671 kb on + strand, within Shewana3_0529 at 623.677 kb on - strand, within Shewana3_0529 at 623.677 kb on - strand, within Shewana3_0529 at 623.677 kb on - strand, within Shewana3_0529 at 623.677 kb on - strand, within Shewana3_0529 at 623.679 kb on - strand, within Shewana3_0529 at 623.679 kb on - strand, within Shewana3_0529 at 623.679 kb on - strand, within Shewana3_0529 at 623.679 kb on - strand, within Shewana3_0529 at 623.679 kb on - strand, within Shewana3_0529 at 623.679 kb on - strand, within Shewana3_0529 at 623.785 kb on + strand, within Shewana3_0529 at 623.793 kb on - strand, within Shewana3_0529 at 623.799 kb on + strand, within Shewana3_0529 at 623.799 kb on + strand, within Shewana3_0529 at 623.800 kb on - strand, within Shewana3_0529 at 623.807 kb on - strand, within Shewana3_0529 at 623.830 kb on + strand, within Shewana3_0529 at 623.889 kb on + strand, within Shewana3_0529 at 623.890 kb on + strand, within Shewana3_0529 at 623.893 kb on - strand, within Shewana3_0529 at 623.899 kb on + strand, within Shewana3_0529 at 623.899 kb on + strand, within Shewana3_0529 at 623.899 kb on - strand, within Shewana3_0529 at 623.907 kb on - strand, within Shewana3_0529 at 623.907 kb on - strand, within Shewana3_0529 at 623.907 kb on - strand, within Shewana3_0529 at 623.907 kb on - strand, within Shewana3_0529 at 623.921 kb on - strand, within Shewana3_0529 at 624.001 kb on + strand, within Shewana3_0529 at 624.008 kb on + strand, within Shewana3_0529 at 624.010 kb on + strand, within Shewana3_0529 at 624.010 kb on + strand, within Shewana3_0529 at 624.010 kb on + strand, within Shewana3_0529 at 624.010 kb on + strand, within Shewana3_0529 at 624.010 kb on + strand, within Shewana3_0529 at 624.010 kb on + strand, within Shewana3_0529 at 624.018 kb on - strand at 624.018 kb on - strand at 624.018 kb on - strand at 624.018 kb on - strand at 624.102 kb on - strand at 624.116 kb on + strand at 624.168 kb on + strand at 624.183 kb on + strand at 624.183 kb on + strand at 624.183 kb on + strand at 624.183 kb on + strand at 624.183 kb on + strand at 624.183 kb on + strand at 624.183 kb on + strand at 624.183 kb on + strand at 624.184 kb on + strand at 624.184 kb on + strand at 624.191 kb on - strand at 624.191 kb on - strand at 624.191 kb on - strand at 624.191 kb on - strand at 624.191 kb on - strand at 624.191 kb on - strand at 624.191 kb on - strand at 624.191 kb on - strand at 624.191 kb on - strand at 624.191 kb on - strand at 624.192 kb on - strand at 624.200 kb on + strand at 624.234 kb on + strand at 624.237 kb on - strand at 624.249 kb on + strand at 624.257 kb on - strand at 624.257 kb on - strand at 624.272 kb on - strand at 624.288 kb on - strand at 624.330 kb on + strand at 624.330 kb on + strand at 624.330 kb on + strand at 624.330 kb on + strand at 624.331 kb on + strand at 624.340 kb on + strand at 624.340 kb on + strand at 624.340 kb on + strand at 624.348 kb on - strand at 624.348 kb on - strand at 624.348 kb on - strand at 624.368 kb on + strand at 624.376 kb on - strand at 624.376 kb on - strand at 624.376 kb on - strand at 624.376 kb on - strand at 624.435 kb on + strand, within Shewana3_0530 at 624.659 kb on + strand, within Shewana3_0530 at 624.923 kb on + strand, within Shewana3_0530 at 624.956 kb on + strand, within Shewana3_0530 at 625.034 kb on + strand, within Shewana3_0530 at 625.043 kb on - strand, within Shewana3_0530
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 622,630 + +0.3 622,631 + +0.2 622,631 + -0.6 622,631 + +1.0 622,631 + +0.6 622,638 - -0.6 622,639 - +0.1 622,639 - -0.2 622,639 - -2.2 622,639 - -0.5 622,658 + +0.2 622,666 - -0.2 622,666 - -0.9 622,668 + +0.7 622,682 - -0.1 622,687 - -0.3 622,693 - -1.2 622,699 + +0.6 622,700 + -0.1 622,700 + -1.2 622,723 + -0.8 622,745 + +0.2 622,745 + +0.0 622,745 + +0.8 622,745 + -0.1 622,745 + +0.3 622,745 + +0.2 622,745 + +1.3 622,753 - -3.0 622,753 - -0.7 622,753 - -1.5 622,753 - -0.5 622,753 - +0.2 622,753 - -1.3 622,753 - -2.1 622,805 + -0.2 622,822 - +0.0 622,822 - +0.1 622,832 + +0.2 622,838 + -0.1 622,838 + +0.8 622,838 + +0.1 622,838 + +0.6 622,838 + -1.0 622,838 + +0.3 622,838 + -0.1 622,838 + +0.5 622,838 + +0.0 622,838 + +0.3 622,840 + +0.0 622,840 - -0.9 622,846 - -1.5 622,846 - -0.5 622,846 - -1.5 622,846 - -1.2 622,846 - -1.7 622,846 - -0.7 622,846 - -2.7 622,846 - -1.8 622,848 - +0.2 622,848 - -1.2 622,850 + -0.3 622,858 - +0.2 622,907 + +0.6 622,913 + -0.3 622,955 - -0.3 622,964 + -0.3 622,974 + +1.2 622,974 + +0.6 622,980 - -0.6 622,996 + -0.7 622,998 + +0.7 623,004 - +0.6 623,004 - -1.1 623,004 - +0.5 623,030 + +0.9 623,065 + -0.3 623,068 - +0.3 623,073 - +0.3 623,082 + -0.9 623,082 + +1.1 623,082 + -1.2 623,084 + -1.9 623,084 + +1.3 623,084 + -0.4 623,084 + -0.7 623,090 - +0.7 623,104 + -0.6 623,108 - +0.4 623,124 - +0.2 623,142 + -0.8 623,176 - +0.9 623,188 + -0.0 623,188 + -0.3 623,205 + -0.2 623,205 + -1.5 623,205 + -0.7 623,213 - +0.2 623,213 - +1.2 623,215 + -0.4 623,276 + -0.5 623,276 + -0.2 623,276 + -0.2 623,282 + -2.6 623,284 - -1.1 623,284 - -1.8 623,284 - -1.8 623,284 - +0.0 623,285 - -1.0 623,285 - -2.9 623,285 - -0.5 623,285 - -1.4 623,285 - -1.2 623,295 - -1.0 623,322 + -1.7 623,322 + -2.6 623,362 - Shewana3_0529 0.12 -2.5 623,376 + Shewana3_0529 0.14 +0.2 623,376 + Shewana3_0529 0.14 +0.3 623,376 + Shewana3_0529 0.14 +0.6 623,376 + Shewana3_0529 0.14 -0.0 623,384 - Shewana3_0529 0.15 -1.4 623,384 - Shewana3_0529 0.15 +0.2 623,384 - Shewana3_0529 0.15 -1.8 623,386 + Shewana3_0529 0.15 -1.5 623,386 + Shewana3_0529 0.15 +0.8 623,386 + Shewana3_0529 0.15 -2.0 623,386 + Shewana3_0529 0.15 -0.2 623,386 + Shewana3_0529 0.15 -1.2 623,386 + Shewana3_0529 0.15 -1.0 623,386 + Shewana3_0529 0.15 +1.4 623,386 + Shewana3_0529 0.15 +1.2 623,386 + Shewana3_0529 0.15 -0.5 623,386 + Shewana3_0529 0.15 -1.1 623,394 - Shewana3_0529 0.16 -1.0 623,394 - Shewana3_0529 0.16 -1.2 623,394 - Shewana3_0529 0.16 -2.4 623,394 - Shewana3_0529 0.16 -0.5 623,394 - Shewana3_0529 0.16 -0.2 623,394 - Shewana3_0529 0.16 +0.5 623,397 + Shewana3_0529 0.16 +0.8 623,397 + Shewana3_0529 0.16 -0.8 623,397 + Shewana3_0529 0.16 -2.4 623,405 - Shewana3_0529 0.17 -0.9 623,421 + Shewana3_0529 0.19 +1.8 623,421 + Shewana3_0529 0.19 -2.8 623,421 + Shewana3_0529 0.19 -0.0 623,430 + Shewana3_0529 0.20 -1.2 623,433 + Shewana3_0529 0.21 -0.8 623,468 + Shewana3_0529 0.25 -0.7 623,468 + Shewana3_0529 0.25 -0.5 623,476 - Shewana3_0529 0.26 +0.8 623,476 - Shewana3_0529 0.26 +0.2 623,477 + Shewana3_0529 0.26 +0.2 623,488 - Shewana3_0529 0.27 -0.7 623,491 + Shewana3_0529 0.28 -2.5 623,491 + Shewana3_0529 0.28 -1.3 623,491 + Shewana3_0529 0.28 -1.9 623,499 - Shewana3_0529 0.29 +0.8 623,499 - Shewana3_0529 0.29 -1.9 623,499 - Shewana3_0529 0.29 +0.8 623,499 - Shewana3_0529 0.29 -0.5 623,501 + Shewana3_0529 0.29 -2.2 623,567 + Shewana3_0529 0.37 -0.5 623,567 + Shewana3_0529 0.37 -1.7 623,567 + Shewana3_0529 0.37 -0.5 623,598 - Shewana3_0529 0.40 -1.2 623,659 + Shewana3_0529 0.48 -1.7 623,667 - Shewana3_0529 0.49 -1.0 623,667 - Shewana3_0529 0.49 +0.2 623,669 + Shewana3_0529 0.49 -1.8 623,669 + Shewana3_0529 0.49 -1.4 623,669 + Shewana3_0529 0.49 -0.8 623,669 + Shewana3_0529 0.49 +0.8 623,671 + Shewana3_0529 0.49 -1.8 623,671 + Shewana3_0529 0.49 -0.5 623,671 + Shewana3_0529 0.49 -1.6 623,671 + Shewana3_0529 0.49 +0.8 623,671 + Shewana3_0529 0.49 -0.5 623,671 + Shewana3_0529 0.49 -0.8 623,671 + Shewana3_0529 0.49 -1.6 623,677 - Shewana3_0529 0.50 -0.2 623,677 - Shewana3_0529 0.50 -1.4 623,677 - Shewana3_0529 0.50 -1.0 623,677 - Shewana3_0529 0.50 -0.2 623,679 - Shewana3_0529 0.50 -2.4 623,679 - Shewana3_0529 0.50 -2.8 623,679 - Shewana3_0529 0.50 -1.7 623,679 - Shewana3_0529 0.50 -0.8 623,679 - Shewana3_0529 0.50 -1.2 623,679 - Shewana3_0529 0.50 +0.2 623,785 + Shewana3_0529 0.63 -1.3 623,793 - Shewana3_0529 0.64 -0.2 623,799 + Shewana3_0529 0.64 +0.8 623,799 + Shewana3_0529 0.64 -0.5 623,800 - Shewana3_0529 0.64 -0.8 623,807 - Shewana3_0529 0.65 -0.8 623,830 + Shewana3_0529 0.68 -0.2 623,889 + Shewana3_0529 0.75 -2.2 623,890 + Shewana3_0529 0.75 +0.4 623,893 - Shewana3_0529 0.75 -1.8 623,899 + Shewana3_0529 0.76 -0.4 623,899 + Shewana3_0529 0.76 -1.9 623,899 - Shewana3_0529 0.76 -0.2 623,907 - Shewana3_0529 0.77 -0.3 623,907 - Shewana3_0529 0.77 -0.9 623,907 - Shewana3_0529 0.77 +0.5 623,907 - Shewana3_0529 0.77 -0.8 623,921 - Shewana3_0529 0.79 +1.1 624,001 + Shewana3_0529 0.88 +0.6 624,008 + Shewana3_0529 0.89 -0.5 624,010 + Shewana3_0529 0.89 -0.5 624,010 + Shewana3_0529 0.89 -1.4 624,010 + Shewana3_0529 0.89 -0.9 624,010 + Shewana3_0529 0.89 -1.2 624,010 + Shewana3_0529 0.89 +0.3 624,010 + Shewana3_0529 0.89 -0.4 624,018 - -1.1 624,018 - -1.0 624,018 - -1.2 624,018 - -0.2 624,102 - -0.3 624,116 + -1.8 624,168 + -0.9 624,183 + -0.2 624,183 + +0.1 624,183 + +0.6 624,183 + -1.2 624,183 + -0.6 624,183 + -1.7 624,183 + -0.9 624,183 + -1.6 624,184 + -1.2 624,184 + +0.3 624,191 - -1.4 624,191 - -1.4 624,191 - -2.3 624,191 - -1.0 624,191 - -0.2 624,191 - -0.6 624,191 - -1.3 624,191 - -2.5 624,191 - -0.5 624,191 - -2.6 624,192 - +0.6 624,200 + -1.4 624,234 + -1.4 624,237 - -0.4 624,249 + -0.1 624,257 - -1.4 624,257 - -0.5 624,272 - -0.6 624,288 - +0.2 624,330 + -0.0 624,330 + -0.2 624,330 + -0.4 624,330 + -2.3 624,331 + +0.6 624,340 + -1.3 624,340 + -0.2 624,340 + +0.9 624,348 - -0.4 624,348 - -0.5 624,348 - -1.9 624,368 + -1.4 624,376 - -0.9 624,376 - -0.2 624,376 - -0.1 624,376 - -0.2 624,435 + Shewana3_0530 0.12 -0.7 624,659 + Shewana3_0530 0.38 -1.3 624,923 + Shewana3_0530 0.68 -2.2 624,956 + Shewana3_0530 0.72 +1.3 625,034 + Shewana3_0530 0.81 -0.1 625,043 - Shewana3_0530 0.82 +0.3
Or see this region's nucleotide sequence