Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0492 and Shewana3_0493 are separated by 86 nucleotides Shewana3_0493 and Shewana3_0494 are separated by 2 nucleotides Shewana3_0494 and Shewana3_0495 are separated by 119 nucleotides Shewana3_0495 and Shewana3_0496 are separated by 9 nucleotides
Shewana3_0492: Shewana3_0492 - formate-dependent nitrite reductase, NrfG protein (RefSeq), at 575,461 to 576,135
_0492
Shewana3_0493: Shewana3_0493 - 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein (RefSeq), at 576,222 to 576,929
_0493
Shewana3_0494: Shewana3_0494 - polysulphide reductase, NrfD (RefSeq), at 576,932 to 577,879
_0494
Shewana3_0495: Shewana3_0495 - no description, at 577,999 to 578,470
_0495
Shewana3_0496: Shewana3_0496 - periplasmic copper-binding (RefSeq), at 578,480 to 579,754
_0496
Position (kb)
576
577
578 Strain fitness (log2 ratio)
-1
0
1 at 576.084 kb on + strand at 576.092 kb on - strand at 576.274 kb on - strand at 576.476 kb on + strand, within Shewana3_0493 at 576.509 kb on - strand, within Shewana3_0493 at 576.816 kb on + strand, within Shewana3_0493 at 576.824 kb on - strand, within Shewana3_0493 at 576.824 kb on - strand, within Shewana3_0493 at 576.893 kb on - strand at 577.039 kb on - strand, within Shewana3_0494 at 577.128 kb on + strand, within Shewana3_0494 at 577.393 kb on + strand, within Shewana3_0494 at 577.549 kb on - strand, within Shewana3_0494 at 577.559 kb on - strand, within Shewana3_0494 at 577.884 kb on + strand at 578.142 kb on + strand, within Shewana3_0495 at 578.268 kb on + strand, within Shewana3_0495 at 578.350 kb on - strand, within Shewana3_0495 at 578.366 kb on - strand, within Shewana3_0495 at 578.727 kb on + strand, within Shewana3_0496
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 576,084 + -0.3 576,092 - -0.1 576,274 - -0.3 576,476 + Shewana3_0493 0.36 +0.7 576,509 - Shewana3_0493 0.41 +0.6 576,816 + Shewana3_0493 0.84 +0.6 576,824 - Shewana3_0493 0.85 -0.6 576,824 - Shewana3_0493 0.85 -0.6 576,893 - +0.2 577,039 - Shewana3_0494 0.11 -0.0 577,128 + Shewana3_0494 0.21 +1.7 577,393 + Shewana3_0494 0.49 +1.6 577,549 - Shewana3_0494 0.65 +0.2 577,559 - Shewana3_0494 0.66 -0.7 577,884 + +0.0 578,142 + Shewana3_0495 0.30 -1.6 578,268 + Shewana3_0495 0.57 -0.6 578,350 - Shewana3_0495 0.74 -0.4 578,366 - Shewana3_0495 0.78 -1.0 578,727 + Shewana3_0496 0.19 +1.1
Or see this region's nucleotide sequence