Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0462 and Shewana3_0463 are separated by 30 nucleotides Shewana3_0463 and Shewana3_0464 are separated by 291 nucleotides Shewana3_0464 and Shewana3_0465 are separated by 655 nucleotides
Shewana3_0462: Shewana3_0462 - protein of unknown function, zinc metallopeptidase putative (RefSeq), at 536,825 to 537,703
_0462
Shewana3_0463: Shewana3_0463 - NUDIX hydrolase (RefSeq), at 537,734 to 538,141
_0463
Shewana3_0464: Shewana3_0464 - hypothetical protein (RefSeq), at 538,433 to 538,771
_0464
Shewana3_0465: Shewana3_0465 - hypothetical protein (RefSeq), at 539,427 to 540,026
_0465
Position (kb) 538
539 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 537.508 kb on + strand, within Shewana3_0462 at 537.516 kb on - strand, within Shewana3_0462 at 537.601 kb on - strand, within Shewana3_0462 at 537.606 kb on + strand, within Shewana3_0462 at 537.607 kb on - strand, within Shewana3_0462 at 537.721 kb on - strand at 537.765 kb on + strand at 537.805 kb on + strand, within Shewana3_0463 at 537.805 kb on + strand, within Shewana3_0463 at 537.805 kb on + strand, within Shewana3_0463 at 537.852 kb on + strand, within Shewana3_0463 at 538.026 kb on + strand, within Shewana3_0463 at 538.034 kb on - strand, within Shewana3_0463 at 538.065 kb on + strand, within Shewana3_0463 at 538.065 kb on + strand, within Shewana3_0463 at 538.073 kb on - strand, within Shewana3_0463 at 538.073 kb on - strand, within Shewana3_0463 at 538.143 kb on - strand at 538.173 kb on + strand at 538.233 kb on + strand at 538.233 kb on + strand at 538.233 kb on + strand at 538.242 kb on - strand at 538.326 kb on + strand at 538.505 kb on + strand, within Shewana3_0464 at 538.530 kb on + strand, within Shewana3_0464 at 538.530 kb on + strand, within Shewana3_0464 at 538.530 kb on + strand, within Shewana3_0464 at 538.530 kb on + strand, within Shewana3_0464 at 538.530 kb on + strand, within Shewana3_0464 at 538.538 kb on - strand, within Shewana3_0464 at 538.538 kb on - strand, within Shewana3_0464 at 538.538 kb on - strand, within Shewana3_0464 at 538.538 kb on - strand, within Shewana3_0464 at 538.538 kb on - strand, within Shewana3_0464 at 538.538 kb on - strand, within Shewana3_0464 at 538.572 kb on + strand, within Shewana3_0464 at 538.572 kb on - strand, within Shewana3_0464 at 538.578 kb on + strand, within Shewana3_0464 at 538.647 kb on + strand, within Shewana3_0464 at 538.807 kb on + strand at 538.863 kb on - strand at 538.978 kb on + strand at 538.995 kb on + strand at 539.043 kb on + strand at 539.043 kb on + strand at 539.043 kb on + strand at 539.043 kb on + strand at 539.043 kb on + strand at 539.044 kb on + strand at 539.052 kb on - strand at 539.052 kb on - strand at 539.119 kb on - strand at 539.318 kb on + strand at 539.416 kb on - strand at 539.437 kb on - strand at 539.438 kb on - strand at 539.440 kb on + strand at 539.440 kb on + strand at 539.464 kb on - strand at 539.497 kb on + strand, within Shewana3_0465 at 539.497 kb on + strand, within Shewana3_0465 at 539.505 kb on - strand, within Shewana3_0465 at 539.507 kb on + strand, within Shewana3_0465 at 539.616 kb on - strand, within Shewana3_0465 at 539.618 kb on + strand, within Shewana3_0465 at 539.640 kb on - strand, within Shewana3_0465 at 539.640 kb on - strand, within Shewana3_0465 at 539.640 kb on - strand, within Shewana3_0465 at 539.650 kb on + strand, within Shewana3_0465 at 539.652 kb on + strand, within Shewana3_0465 at 539.660 kb on - strand, within Shewana3_0465 at 539.676 kb on + strand, within Shewana3_0465 at 539.759 kb on - strand, within Shewana3_0465
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 537,508 + Shewana3_0462 0.78 +1.4 537,516 - Shewana3_0462 0.79 -0.2 537,601 - Shewana3_0462 0.88 +1.7 537,606 + Shewana3_0462 0.89 -0.6 537,607 - Shewana3_0462 0.89 -3.3 537,721 - -0.9 537,765 + -1.3 537,805 + Shewana3_0463 0.17 -1.1 537,805 + Shewana3_0463 0.17 -1.0 537,805 + Shewana3_0463 0.17 +0.0 537,852 + Shewana3_0463 0.29 -1.2 538,026 + Shewana3_0463 0.72 -0.0 538,034 - Shewana3_0463 0.74 -1.5 538,065 + Shewana3_0463 0.81 +0.8 538,065 + Shewana3_0463 0.81 -1.1 538,073 - Shewana3_0463 0.83 +0.7 538,073 - Shewana3_0463 0.83 -1.3 538,143 - +0.4 538,173 + -1.4 538,233 + +1.3 538,233 + -1.2 538,233 + -2.0 538,242 - -1.2 538,326 + +0.5 538,505 + Shewana3_0464 0.21 -0.6 538,530 + Shewana3_0464 0.29 -0.2 538,530 + Shewana3_0464 0.29 -0.0 538,530 + Shewana3_0464 0.29 +0.8 538,530 + Shewana3_0464 0.29 -1.1 538,530 + Shewana3_0464 0.29 -2.0 538,538 - Shewana3_0464 0.31 +0.2 538,538 - Shewana3_0464 0.31 -1.4 538,538 - Shewana3_0464 0.31 -0.3 538,538 - Shewana3_0464 0.31 -0.7 538,538 - Shewana3_0464 0.31 +0.1 538,538 - Shewana3_0464 0.31 -1.0 538,572 + Shewana3_0464 0.41 -1.8 538,572 - Shewana3_0464 0.41 -2.3 538,578 + Shewana3_0464 0.43 -1.0 538,647 + Shewana3_0464 0.63 -0.1 538,807 + +0.4 538,863 - -0.4 538,978 + -0.5 538,995 + -0.3 539,043 + +0.3 539,043 + -0.7 539,043 + -0.3 539,043 + -0.1 539,043 + -1.7 539,044 + -0.2 539,052 - -1.9 539,052 - -1.8 539,119 - -0.2 539,318 + -0.2 539,416 - +0.3 539,437 - +0.2 539,438 - +0.3 539,440 + -0.5 539,440 + -0.2 539,464 - +0.7 539,497 + Shewana3_0465 0.12 -0.5 539,497 + Shewana3_0465 0.12 +0.2 539,505 - Shewana3_0465 0.13 +0.4 539,507 + Shewana3_0465 0.13 -0.2 539,616 - Shewana3_0465 0.32 +0.0 539,618 + Shewana3_0465 0.32 -1.5 539,640 - Shewana3_0465 0.35 +0.5 539,640 - Shewana3_0465 0.35 -1.4 539,640 - Shewana3_0465 0.35 -0.3 539,650 + Shewana3_0465 0.37 -0.8 539,652 + Shewana3_0465 0.38 -0.2 539,660 - Shewana3_0465 0.39 +0.1 539,676 + Shewana3_0465 0.41 -1.0 539,759 - Shewana3_0465 0.55 +0.8
Or see this region's nucleotide sequence