Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0459 and Shewana3_0460 are separated by 193 nucleotides Shewana3_0460 and Shewana3_0461 are separated by 11 nucleotides Shewana3_0461 and Shewana3_0462 are separated by 47 nucleotides Shewana3_0462 and Shewana3_0463 are separated by 30 nucleotides Shewana3_0463 and Shewana3_0464 are separated by 291 nucleotides
Shewana3_0459: Shewana3_0459 - protein of unknown function DUF853, NPT hydrolase putative (RefSeq), at 534,420 to 535,913
_0459
Shewana3_0460: Shewana3_0460 - hypothetical protein (RefSeq), at 536,107 to 536,340
_0460
Shewana3_0461: Shewana3_0461 - hypothetical protein (RefSeq), at 536,352 to 536,777
_0461
Shewana3_0462: Shewana3_0462 - protein of unknown function, zinc metallopeptidase putative (RefSeq), at 536,825 to 537,703
_0462
Shewana3_0463: Shewana3_0463 - NUDIX hydrolase (RefSeq), at 537,734 to 538,141
_0463
Shewana3_0464: Shewana3_0464 - hypothetical protein (RefSeq), at 538,433 to 538,771
_0464
Position (kb) 536
537
538 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 535.907 kb on - strand at 535.961 kb on + strand at 535.969 kb on - strand at 535.969 kb on - strand at 536.050 kb on - strand at 536.051 kb on - strand at 536.056 kb on - strand at 536.154 kb on - strand, within Shewana3_0460 at 536.245 kb on + strand, within Shewana3_0460 at 536.253 kb on - strand, within Shewana3_0460 at 536.253 kb on - strand, within Shewana3_0460 at 536.316 kb on - strand, within Shewana3_0460 at 536.351 kb on + strand at 536.375 kb on + strand at 536.414 kb on - strand, within Shewana3_0461 at 536.813 kb on - strand at 536.881 kb on + strand at 536.881 kb on - strand at 537.015 kb on + strand, within Shewana3_0462 at 537.015 kb on + strand, within Shewana3_0462 at 537.207 kb on + strand, within Shewana3_0462 at 537.240 kb on + strand, within Shewana3_0462 at 537.356 kb on + strand, within Shewana3_0462 at 537.386 kb on - strand, within Shewana3_0462 at 537.508 kb on + strand, within Shewana3_0462 at 537.516 kb on - strand, within Shewana3_0462 at 537.601 kb on - strand, within Shewana3_0462 at 537.606 kb on + strand, within Shewana3_0462 at 537.607 kb on - strand, within Shewana3_0462 at 537.721 kb on - strand at 537.765 kb on + strand at 537.805 kb on + strand, within Shewana3_0463 at 537.805 kb on + strand, within Shewana3_0463 at 537.805 kb on + strand, within Shewana3_0463 at 537.852 kb on + strand, within Shewana3_0463 at 538.026 kb on + strand, within Shewana3_0463 at 538.034 kb on - strand, within Shewana3_0463 at 538.065 kb on + strand, within Shewana3_0463 at 538.065 kb on + strand, within Shewana3_0463 at 538.073 kb on - strand, within Shewana3_0463 at 538.073 kb on - strand, within Shewana3_0463 at 538.143 kb on - strand at 538.173 kb on + strand at 538.233 kb on + strand at 538.233 kb on + strand at 538.233 kb on + strand at 538.242 kb on - strand at 538.326 kb on + strand at 538.505 kb on + strand, within Shewana3_0464 at 538.530 kb on + strand, within Shewana3_0464 at 538.530 kb on + strand, within Shewana3_0464 at 538.530 kb on + strand, within Shewana3_0464 at 538.530 kb on + strand, within Shewana3_0464 at 538.530 kb on + strand, within Shewana3_0464 at 538.538 kb on - strand, within Shewana3_0464 at 538.538 kb on - strand, within Shewana3_0464 at 538.538 kb on - strand, within Shewana3_0464 at 538.538 kb on - strand, within Shewana3_0464 at 538.538 kb on - strand, within Shewana3_0464 at 538.538 kb on - strand, within Shewana3_0464 at 538.572 kb on + strand, within Shewana3_0464 at 538.572 kb on - strand, within Shewana3_0464 at 538.578 kb on + strand, within Shewana3_0464 at 538.647 kb on + strand, within Shewana3_0464
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 535,907 - -1.2 535,961 + -0.1 535,969 - -0.5 535,969 - +0.4 536,050 - -2.6 536,051 - -0.3 536,056 - +1.0 536,154 - Shewana3_0460 0.20 +0.0 536,245 + Shewana3_0460 0.59 -0.2 536,253 - Shewana3_0460 0.62 -0.1 536,253 - Shewana3_0460 0.62 +0.3 536,316 - Shewana3_0460 0.89 -0.1 536,351 + +1.1 536,375 + +0.5 536,414 - Shewana3_0461 0.15 +0.6 536,813 - +0.1 536,881 + -1.0 536,881 - -2.6 537,015 + Shewana3_0462 0.22 +0.2 537,015 + Shewana3_0462 0.22 -0.5 537,207 + Shewana3_0462 0.43 +0.6 537,240 + Shewana3_0462 0.47 -0.1 537,356 + Shewana3_0462 0.60 +1.1 537,386 - Shewana3_0462 0.64 +0.3 537,508 + Shewana3_0462 0.78 +1.4 537,516 - Shewana3_0462 0.79 -0.2 537,601 - Shewana3_0462 0.88 +1.7 537,606 + Shewana3_0462 0.89 -0.6 537,607 - Shewana3_0462 0.89 -3.3 537,721 - -0.9 537,765 + -1.3 537,805 + Shewana3_0463 0.17 -1.1 537,805 + Shewana3_0463 0.17 -1.0 537,805 + Shewana3_0463 0.17 +0.0 537,852 + Shewana3_0463 0.29 -1.2 538,026 + Shewana3_0463 0.72 -0.0 538,034 - Shewana3_0463 0.74 -1.5 538,065 + Shewana3_0463 0.81 +0.8 538,065 + Shewana3_0463 0.81 -1.1 538,073 - Shewana3_0463 0.83 +0.7 538,073 - Shewana3_0463 0.83 -1.3 538,143 - +0.4 538,173 + -1.4 538,233 + +1.3 538,233 + -1.2 538,233 + -2.0 538,242 - -1.2 538,326 + +0.5 538,505 + Shewana3_0464 0.21 -0.6 538,530 + Shewana3_0464 0.29 -0.2 538,530 + Shewana3_0464 0.29 -0.0 538,530 + Shewana3_0464 0.29 +0.8 538,530 + Shewana3_0464 0.29 -1.1 538,530 + Shewana3_0464 0.29 -2.0 538,538 - Shewana3_0464 0.31 +0.2 538,538 - Shewana3_0464 0.31 -1.4 538,538 - Shewana3_0464 0.31 -0.3 538,538 - Shewana3_0464 0.31 -0.7 538,538 - Shewana3_0464 0.31 +0.1 538,538 - Shewana3_0464 0.31 -1.0 538,572 + Shewana3_0464 0.41 -1.8 538,572 - Shewana3_0464 0.41 -2.3 538,578 + Shewana3_0464 0.43 -1.0 538,647 + Shewana3_0464 0.63 -0.1
Or see this region's nucleotide sequence