Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0451 and Shewana3_0452 are separated by 63 nucleotides Shewana3_0452 and Shewana3_0453 are separated by 189 nucleotides Shewana3_0453 and Shewana3_0454 are separated by 164 nucleotides
Shewana3_0451: Shewana3_0451 - anion transporter (RefSeq), at 526,350 to 527,750
_0451
Shewana3_0452: Shewana3_0452 - thioredoxin (RefSeq), at 527,814 to 528,236
_0452
Shewana3_0453: Shewana3_0453 - peptidylprolyl isomerase, FKBP-type (RefSeq), at 528,426 to 528,761
_0453
Shewana3_0454: Shewana3_0454 - diguanylate cyclase with PAS/PAC sensor (RefSeq), at 528,926 to 529,912
_0454
Position (kb) 527
528
529 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 526.875 kb on + strand, within Shewana3_0451 at 527.201 kb on + strand, within Shewana3_0451 at 527.280 kb on - strand, within Shewana3_0451 at 527.352 kb on - strand, within Shewana3_0451 at 527.541 kb on + strand, within Shewana3_0451 at 527.549 kb on + strand, within Shewana3_0451 at 527.617 kb on - strand at 527.652 kb on - strand at 527.712 kb on + strand at 527.722 kb on + strand at 527.745 kb on + strand at 527.754 kb on + strand at 527.754 kb on + strand at 527.762 kb on - strand at 527.762 kb on - strand at 527.762 kb on - strand at 527.967 kb on - strand, within Shewana3_0452 at 528.209 kb on - strand at 528.311 kb on + strand at 528.311 kb on + strand at 528.311 kb on + strand at 528.319 kb on - strand at 528.319 kb on - strand at 528.541 kb on - strand, within Shewana3_0453 at 528.663 kb on + strand, within Shewana3_0453 at 528.717 kb on - strand, within Shewana3_0453 at 528.787 kb on + strand at 528.787 kb on + strand at 528.818 kb on - strand at 528.887 kb on - strand at 529.103 kb on - strand, within Shewana3_0454 at 529.103 kb on - strand, within Shewana3_0454 at 529.105 kb on + strand, within Shewana3_0454 at 529.113 kb on - strand, within Shewana3_0454 at 529.113 kb on - strand, within Shewana3_0454 at 529.195 kb on + strand, within Shewana3_0454
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 526,875 + Shewana3_0451 0.37 +0.9 527,201 + Shewana3_0451 0.61 +0.8 527,280 - Shewana3_0451 0.66 +0.6 527,352 - Shewana3_0451 0.72 -0.8 527,541 + Shewana3_0451 0.85 +0.4 527,549 + Shewana3_0451 0.86 +0.5 527,617 - -0.5 527,652 - -1.5 527,712 + +1.1 527,722 + +0.7 527,745 + +0.8 527,754 + +0.8 527,754 + +0.4 527,762 - +1.0 527,762 - -0.9 527,762 - -1.8 527,967 - Shewana3_0452 0.36 +0.8 528,209 - +1.6 528,311 + +0.3 528,311 + +0.0 528,311 + +0.5 528,319 - +0.8 528,319 - +0.1 528,541 - Shewana3_0453 0.34 -2.9 528,663 + Shewana3_0453 0.71 +0.7 528,717 - Shewana3_0453 0.87 -0.4 528,787 + -2.2 528,787 + -1.0 528,818 - -0.4 528,887 - +0.2 529,103 - Shewana3_0454 0.18 -3.4 529,103 - Shewana3_0454 0.18 -0.2 529,105 + Shewana3_0454 0.18 -0.7 529,113 - Shewana3_0454 0.19 -0.0 529,113 - Shewana3_0454 0.19 -0.3 529,195 + Shewana3_0454 0.27 +0.6
Or see this region's nucleotide sequence