Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0449 and Shewana3_0450 are separated by 333 nucleotides Shewana3_0450 and Shewana3_0451 are separated by 84 nucleotides Shewana3_0451 and Shewana3_0452 are separated by 63 nucleotides Shewana3_0452 and Shewana3_0453 are separated by 189 nucleotides
Shewana3_0449: Shewana3_0449 - diguanylate cyclase with GAF sensor (RefSeq), at 524,652 to 525,695
_0449
Shewana3_0450: Shewana3_0450 - no description, at 526,029 to 526,265
_0450
Shewana3_0451: Shewana3_0451 - anion transporter (RefSeq), at 526,350 to 527,750
_0451
Shewana3_0452: Shewana3_0452 - thioredoxin (RefSeq), at 527,814 to 528,236
_0452
Shewana3_0453: Shewana3_0453 - peptidylprolyl isomerase, FKBP-type (RefSeq), at 528,426 to 528,761
_0453
Position (kb) 526
527
528 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 525.368 kb on - strand, within Shewana3_0449 at 525.393 kb on + strand, within Shewana3_0449 at 525.425 kb on + strand, within Shewana3_0449 at 525.516 kb on + strand, within Shewana3_0449 at 525.527 kb on + strand, within Shewana3_0449 at 525.549 kb on - strand, within Shewana3_0449 at 525.657 kb on - strand at 525.668 kb on + strand at 525.705 kb on + strand at 525.712 kb on + strand at 526.090 kb on + strand, within Shewana3_0450 at 526.463 kb on + strand at 526.471 kb on - strand at 526.508 kb on - strand, within Shewana3_0451 at 526.517 kb on - strand, within Shewana3_0451 at 526.542 kb on - strand, within Shewana3_0451 at 526.594 kb on + strand, within Shewana3_0451 at 526.875 kb on + strand, within Shewana3_0451 at 527.201 kb on + strand, within Shewana3_0451 at 527.280 kb on - strand, within Shewana3_0451 at 527.352 kb on - strand, within Shewana3_0451 at 527.541 kb on + strand, within Shewana3_0451 at 527.549 kb on + strand, within Shewana3_0451 at 527.617 kb on - strand at 527.652 kb on - strand at 527.712 kb on + strand at 527.722 kb on + strand at 527.745 kb on + strand at 527.754 kb on + strand at 527.754 kb on + strand at 527.762 kb on - strand at 527.762 kb on - strand at 527.762 kb on - strand at 527.967 kb on - strand, within Shewana3_0452 at 528.209 kb on - strand at 528.311 kb on + strand at 528.311 kb on + strand at 528.311 kb on + strand at 528.319 kb on - strand at 528.319 kb on - strand at 528.541 kb on - strand, within Shewana3_0453 at 528.663 kb on + strand, within Shewana3_0453 at 528.717 kb on - strand, within Shewana3_0453
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 525,368 - Shewana3_0449 0.69 +0.5 525,393 + Shewana3_0449 0.71 -0.1 525,425 + Shewana3_0449 0.74 -0.4 525,516 + Shewana3_0449 0.83 +0.1 525,527 + Shewana3_0449 0.84 -0.0 525,549 - Shewana3_0449 0.86 -0.5 525,657 - +0.1 525,668 + -0.7 525,705 + +2.0 525,712 + +0.4 526,090 + Shewana3_0450 0.26 -0.4 526,463 + -0.1 526,471 - -1.5 526,508 - Shewana3_0451 0.11 -1.0 526,517 - Shewana3_0451 0.12 -1.0 526,542 - Shewana3_0451 0.14 -1.1 526,594 + Shewana3_0451 0.17 +1.2 526,875 + Shewana3_0451 0.37 +0.9 527,201 + Shewana3_0451 0.61 +0.8 527,280 - Shewana3_0451 0.66 +0.6 527,352 - Shewana3_0451 0.72 -0.8 527,541 + Shewana3_0451 0.85 +0.4 527,549 + Shewana3_0451 0.86 +0.5 527,617 - -0.5 527,652 - -1.5 527,712 + +1.1 527,722 + +0.7 527,745 + +0.8 527,754 + +0.8 527,754 + +0.4 527,762 - +1.0 527,762 - -0.9 527,762 - -1.8 527,967 - Shewana3_0452 0.36 +0.8 528,209 - +1.6 528,311 + +0.3 528,311 + +0.0 528,311 + +0.5 528,319 - +0.8 528,319 - +0.1 528,541 - Shewana3_0453 0.34 -2.9 528,663 + Shewana3_0453 0.71 +0.7 528,717 - Shewana3_0453 0.87 -0.4
Or see this region's nucleotide sequence