Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0417 and Shewana3_0418 are separated by 61 nucleotides Shewana3_0418 and Shewana3_0419 are separated by 122 nucleotides
Shewana3_0417: Shewana3_0417 - type 4 prepilin peptidase 1. Aspartic peptidase. MEROPS family A24A (RefSeq), at 474,440 to 475,369
_0417
Shewana3_0418: Shewana3_0418 - type II secretion system protein (RefSeq), at 475,431 to 476,702
_0418
Shewana3_0419: Shewana3_0419 - type IV-A pilus assembly ATPase PilB (RefSeq), at 476,825 to 478,534
_0419
Position (kb) 475
476
477 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 474.637 kb on - strand, within Shewana3_0417 at 474.999 kb on + strand, within Shewana3_0417 at 475.035 kb on - strand, within Shewana3_0417 at 475.133 kb on - strand, within Shewana3_0417 at 475.340 kb on + strand at 475.348 kb on - strand at 475.427 kb on - strand at 475.791 kb on + strand, within Shewana3_0418 at 476.070 kb on + strand, within Shewana3_0418 at 476.091 kb on + strand, within Shewana3_0418 at 476.202 kb on + strand, within Shewana3_0418 at 476.224 kb on + strand, within Shewana3_0418 at 476.229 kb on + strand, within Shewana3_0418 at 476.384 kb on + strand, within Shewana3_0418 at 476.490 kb on - strand, within Shewana3_0418 at 476.515 kb on + strand, within Shewana3_0418 at 476.515 kb on + strand, within Shewana3_0418 at 476.522 kb on + strand, within Shewana3_0418 at 476.589 kb on + strand at 476.807 kb on - strand at 476.902 kb on + strand at 476.912 kb on + strand at 476.913 kb on + strand at 476.920 kb on - strand at 476.920 kb on - strand at 477.054 kb on + strand, within Shewana3_0419 at 477.055 kb on + strand, within Shewana3_0419 at 477.055 kb on - strand, within Shewana3_0419 at 477.063 kb on - strand, within Shewana3_0419 at 477.105 kb on - strand, within Shewana3_0419 at 477.128 kb on + strand, within Shewana3_0419 at 477.238 kb on + strand, within Shewana3_0419 at 477.266 kb on - strand, within Shewana3_0419 at 477.303 kb on + strand, within Shewana3_0419 at 477.356 kb on - strand, within Shewana3_0419 at 477.378 kb on + strand, within Shewana3_0419 at 477.409 kb on - strand, within Shewana3_0419 at 477.409 kb on - strand, within Shewana3_0419 at 477.488 kb on - strand, within Shewana3_0419 at 477.506 kb on + strand, within Shewana3_0419 at 477.506 kb on + strand, within Shewana3_0419 at 477.511 kb on + strand, within Shewana3_0419 at 477.514 kb on - strand, within Shewana3_0419 at 477.514 kb on - strand, within Shewana3_0419 at 477.514 kb on - strand, within Shewana3_0419 at 477.514 kb on - strand, within Shewana3_0419 at 477.514 kb on - strand, within Shewana3_0419 at 477.514 kb on - strand, within Shewana3_0419 at 477.516 kb on + strand, within Shewana3_0419 at 477.516 kb on + strand, within Shewana3_0419 at 477.516 kb on + strand, within Shewana3_0419 at 477.524 kb on - strand, within Shewana3_0419 at 477.524 kb on - strand, within Shewana3_0419 at 477.569 kb on + strand, within Shewana3_0419 at 477.577 kb on - strand, within Shewana3_0419 at 477.700 kb on + strand, within Shewana3_0419
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 474,637 - Shewana3_0417 0.21 -0.8 474,999 + Shewana3_0417 0.60 -1.0 475,035 - Shewana3_0417 0.64 -0.3 475,133 - Shewana3_0417 0.75 -0.4 475,340 + -1.3 475,348 - -0.8 475,427 - -0.3 475,791 + Shewana3_0418 0.28 -0.9 476,070 + Shewana3_0418 0.50 -0.6 476,091 + Shewana3_0418 0.52 -1.6 476,202 + Shewana3_0418 0.61 -0.0 476,224 + Shewana3_0418 0.62 -0.1 476,229 + Shewana3_0418 0.63 -1.1 476,384 + Shewana3_0418 0.75 -2.7 476,490 - Shewana3_0418 0.83 +1.8 476,515 + Shewana3_0418 0.85 +0.1 476,515 + Shewana3_0418 0.85 -1.2 476,522 + Shewana3_0418 0.86 -0.8 476,589 + -1.7 476,807 - +0.2 476,902 + -2.0 476,912 + -0.8 476,913 + -0.2 476,920 - +0.5 476,920 - -0.1 477,054 + Shewana3_0419 0.13 -0.9 477,055 + Shewana3_0419 0.13 -2.4 477,055 - Shewana3_0419 0.13 +0.5 477,063 - Shewana3_0419 0.14 -0.2 477,105 - Shewana3_0419 0.16 -1.0 477,128 + Shewana3_0419 0.18 -1.0 477,238 + Shewana3_0419 0.24 +1.5 477,266 - Shewana3_0419 0.26 -2.0 477,303 + Shewana3_0419 0.28 -0.9 477,356 - Shewana3_0419 0.31 +0.3 477,378 + Shewana3_0419 0.32 -0.7 477,409 - Shewana3_0419 0.34 +0.4 477,409 - Shewana3_0419 0.34 +0.3 477,488 - Shewana3_0419 0.39 +0.5 477,506 + Shewana3_0419 0.40 -0.5 477,506 + Shewana3_0419 0.40 -0.3 477,511 + Shewana3_0419 0.40 -3.3 477,514 - Shewana3_0419 0.40 +0.1 477,514 - Shewana3_0419 0.40 +1.2 477,514 - Shewana3_0419 0.40 +1.6 477,514 - Shewana3_0419 0.40 +0.4 477,514 - Shewana3_0419 0.40 +0.7 477,514 - Shewana3_0419 0.40 +0.5 477,516 + Shewana3_0419 0.40 +0.1 477,516 + Shewana3_0419 0.40 -1.0 477,516 + Shewana3_0419 0.40 -0.3 477,524 - Shewana3_0419 0.41 -0.6 477,524 - Shewana3_0419 0.41 +0.6 477,569 + Shewana3_0419 0.44 -1.9 477,577 - Shewana3_0419 0.44 +0.5 477,700 + Shewana3_0419 0.51 +0.2
Or see this region's nucleotide sequence