Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0361 and Shewana3_0362 are separated by 344 nucleotides Shewana3_0362 and Shewana3_0363 are separated by 267 nucleotides Shewana3_0363 and Shewana3_0364 are separated by 102 nucleotides Shewana3_0364 and Shewana3_0365 are separated by 92 nucleotides
Shewana3_0361: Shewana3_0361 - hypothetical protein (RefSeq), at 398,978 to 399,271
_0361
Shewana3_0362: Shewana3_0362 - hypothetical protein (RefSeq), at 399,616 to 399,855
_0362
Shewana3_0363: Shewana3_0363 - hypothetical protein (RefSeq), at 400,123 to 400,650
_0363
Shewana3_0364: Shewana3_0364 - phosphatidylglycerophosphatase (RefSeq), at 400,753 to 401,550
_0364
Shewana3_0365: Shewana3_0365 - hypothetical protein (RefSeq), at 401,643 to 403,118
_0365
Position (kb) 400
401 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 399.166 kb on - strand, within Shewana3_0361 at 399.295 kb on + strand at 399.850 kb on + strand at 400.289 kb on + strand, within Shewana3_0363 at 400.411 kb on + strand, within Shewana3_0363 at 400.416 kb on + strand, within Shewana3_0363 at 400.416 kb on + strand, within Shewana3_0363 at 400.416 kb on + strand, within Shewana3_0363 at 400.494 kb on + strand, within Shewana3_0363 at 400.731 kb on - strand at 400.855 kb on - strand, within Shewana3_0364 at 400.971 kb on + strand, within Shewana3_0364 at 400.971 kb on + strand, within Shewana3_0364 at 401.116 kb on - strand, within Shewana3_0364 at 401.123 kb on + strand, within Shewana3_0364 at 401.354 kb on + strand, within Shewana3_0364 at 401.394 kb on - strand, within Shewana3_0364 at 401.520 kb on - strand at 401.521 kb on + strand at 401.553 kb on + strand at 401.566 kb on + strand at 401.643 kb on + strand at 401.643 kb on + strand at 401.643 kb on + strand at 401.645 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 399,166 - Shewana3_0361 0.64 -0.4 399,295 + +0.3 399,850 + +0.1 400,289 + Shewana3_0363 0.31 -0.3 400,411 + Shewana3_0363 0.55 -0.1 400,416 + Shewana3_0363 0.55 +0.0 400,416 + Shewana3_0363 0.55 -0.7 400,416 + Shewana3_0363 0.55 -0.4 400,494 + Shewana3_0363 0.70 -1.3 400,731 - +0.1 400,855 - Shewana3_0364 0.13 +0.5 400,971 + Shewana3_0364 0.27 +0.4 400,971 + Shewana3_0364 0.27 +0.5 401,116 - Shewana3_0364 0.45 +0.6 401,123 + Shewana3_0364 0.46 +0.4 401,354 + Shewana3_0364 0.75 -0.2 401,394 - Shewana3_0364 0.80 -1.0 401,520 - -2.9 401,521 + +1.4 401,553 + +0.7 401,566 + -0.1 401,643 + +0.9 401,643 + -0.4 401,643 + -1.2 401,645 + +0.5
Or see this region's nucleotide sequence