Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0352 and Shewana3_0353 are separated by 172 nucleotides Shewana3_0353 and Shewana3_0354 are separated by 59 nucleotides
Shewana3_0351: Shewana3_0351 - hypothetical protein (RefSeq), at 386,284 to 386,697
_0351
Shewana3_0352: Shewana3_0352 - amino acid ABC transporter periplasmic protein (RefSeq), at 386,660 to 387,499
_0352
Shewana3_0353: Shewana3_0353 - hypothetical protein (RefSeq), at 387,672 to 388,988
_0353
Shewana3_0354: Shewana3_0354 - DNA-binding transcriptional regulator IlvY (RefSeq), at 389,048 to 389,920
_0354
Position (kb) 387
388
389 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 386.759 kb on - strand, within Shewana3_0352 at 386.830 kb on + strand, within Shewana3_0352 at 386.887 kb on + strand, within Shewana3_0352 at 386.895 kb on - strand, within Shewana3_0352 at 386.895 kb on - strand, within Shewana3_0352 at 386.895 kb on - strand, within Shewana3_0352 at 387.045 kb on + strand, within Shewana3_0352 at 387.169 kb on - strand, within Shewana3_0352 at 387.242 kb on + strand, within Shewana3_0352 at 387.260 kb on - strand, within Shewana3_0352 at 387.286 kb on + strand, within Shewana3_0352 at 387.286 kb on + strand, within Shewana3_0352 at 387.286 kb on + strand, within Shewana3_0352 at 387.286 kb on + strand, within Shewana3_0352 at 387.294 kb on - strand, within Shewana3_0352 at 387.294 kb on - strand, within Shewana3_0352 at 387.408 kb on - strand, within Shewana3_0352 at 387.427 kb on - strand at 387.467 kb on - strand at 387.472 kb on - strand at 387.803 kb on - strand at 387.958 kb on + strand, within Shewana3_0353 at 387.958 kb on + strand, within Shewana3_0353 at 387.958 kb on + strand, within Shewana3_0353 at 387.966 kb on - strand, within Shewana3_0353 at 387.966 kb on - strand, within Shewana3_0353 at 387.966 kb on - strand, within Shewana3_0353 at 388.177 kb on + strand, within Shewana3_0353 at 388.177 kb on + strand, within Shewana3_0353 at 388.177 kb on + strand, within Shewana3_0353 at 388.246 kb on - strand, within Shewana3_0353 at 388.457 kb on - strand, within Shewana3_0353 at 388.457 kb on - strand, within Shewana3_0353 at 388.552 kb on + strand, within Shewana3_0353 at 388.552 kb on + strand, within Shewana3_0353 at 388.552 kb on + strand, within Shewana3_0353 at 388.552 kb on + strand, within Shewana3_0353 at 388.552 kb on + strand, within Shewana3_0353 at 388.560 kb on - strand, within Shewana3_0353 at 388.560 kb on - strand, within Shewana3_0353 at 388.560 kb on - strand, within Shewana3_0353 at 388.835 kb on - strand, within Shewana3_0353 at 388.985 kb on - strand at 389.099 kb on - strand at 389.199 kb on - strand, within Shewana3_0354 at 389.217 kb on - strand, within Shewana3_0354 at 389.288 kb on - strand, within Shewana3_0354 at 389.399 kb on + strand, within Shewana3_0354 at 389.407 kb on - strand, within Shewana3_0354 at 389.433 kb on + strand, within Shewana3_0354 at 389.689 kb on - strand, within Shewana3_0354 at 389.716 kb on - strand, within Shewana3_0354 at 389.747 kb on + strand, within Shewana3_0354 at 389.755 kb on - strand, within Shewana3_0354 at 389.878 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 386,759 - Shewana3_0352 0.12 -0.8 386,830 + Shewana3_0352 0.20 +0.3 386,887 + Shewana3_0352 0.27 +0.3 386,895 - Shewana3_0352 0.28 -1.1 386,895 - Shewana3_0352 0.28 -0.1 386,895 - Shewana3_0352 0.28 -0.2 387,045 + Shewana3_0352 0.46 -1.3 387,169 - Shewana3_0352 0.61 +0.2 387,242 + Shewana3_0352 0.69 -0.6 387,260 - Shewana3_0352 0.71 -0.7 387,286 + Shewana3_0352 0.75 -1.3 387,286 + Shewana3_0352 0.75 +0.1 387,286 + Shewana3_0352 0.75 -0.0 387,286 + Shewana3_0352 0.75 -0.6 387,294 - Shewana3_0352 0.75 -2.0 387,294 - Shewana3_0352 0.75 +0.1 387,408 - Shewana3_0352 0.89 -3.4 387,427 - -2.1 387,467 - -2.3 387,472 - -1.3 387,803 - -1.2 387,958 + Shewana3_0353 0.22 -0.6 387,958 + Shewana3_0353 0.22 +0.1 387,958 + Shewana3_0353 0.22 +0.5 387,966 - Shewana3_0353 0.22 -1.6 387,966 - Shewana3_0353 0.22 -2.3 387,966 - Shewana3_0353 0.22 -1.0 388,177 + Shewana3_0353 0.38 +0.7 388,177 + Shewana3_0353 0.38 -1.5 388,177 + Shewana3_0353 0.38 +0.6 388,246 - Shewana3_0353 0.44 -0.8 388,457 - Shewana3_0353 0.60 +2.1 388,457 - Shewana3_0353 0.60 -0.3 388,552 + Shewana3_0353 0.67 +0.0 388,552 + Shewana3_0353 0.67 -0.4 388,552 + Shewana3_0353 0.67 -0.7 388,552 + Shewana3_0353 0.67 -0.3 388,552 + Shewana3_0353 0.67 -0.7 388,560 - Shewana3_0353 0.67 -2.5 388,560 - Shewana3_0353 0.67 -1.4 388,560 - Shewana3_0353 0.67 -1.0 388,835 - Shewana3_0353 0.88 -1.6 388,985 - -2.6 389,099 - +0.6 389,199 - Shewana3_0354 0.17 +0.3 389,217 - Shewana3_0354 0.19 +0.1 389,288 - Shewana3_0354 0.27 -0.4 389,399 + Shewana3_0354 0.40 +1.2 389,407 - Shewana3_0354 0.41 -0.4 389,433 + Shewana3_0354 0.44 +0.1 389,689 - Shewana3_0354 0.73 +0.5 389,716 - Shewana3_0354 0.77 +0.3 389,747 + Shewana3_0354 0.80 -1.0 389,755 - Shewana3_0354 0.81 +0.8 389,878 - -0.0
Or see this region's nucleotide sequence