Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0349 and Shewana3_0350 are separated by 16 nucleotides Shewana3_0350 and Shewana3_0351 are separated by 151 nucleotides Shewana3_0351 and Shewana3_0352 overlap by 38 nucleotides
Shewana3_0349: Shewana3_0349 - periplasmic sugar-binding protein, putative (RefSeq), at 383,252 to 384,298
_0349
Shewana3_0350: Shewana3_0350 - diguanylate cyclase (RefSeq), at 384,315 to 386,132
_0350
Shewana3_0351: Shewana3_0351 - hypothetical protein (RefSeq), at 386,284 to 386,697
_0351
Shewana3_0352: Shewana3_0352 - amino acid ABC transporter periplasmic protein (RefSeq), at 386,660 to 387,499
_0352
Position (kb) 384
385
386
387 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 383.335 kb on + strand at 383.383 kb on + strand, within Shewana3_0349 at 383.383 kb on + strand, within Shewana3_0349 at 383.391 kb on - strand, within Shewana3_0349 at 383.406 kb on - strand, within Shewana3_0349 at 383.580 kb on + strand, within Shewana3_0349 at 383.587 kb on - strand, within Shewana3_0349 at 383.587 kb on - strand, within Shewana3_0349 at 383.605 kb on + strand, within Shewana3_0349 at 383.701 kb on - strand, within Shewana3_0349 at 383.741 kb on + strand, within Shewana3_0349 at 383.749 kb on - strand, within Shewana3_0349 at 383.788 kb on + strand, within Shewana3_0349 at 383.796 kb on - strand, within Shewana3_0349 at 383.799 kb on + strand, within Shewana3_0349 at 383.807 kb on - strand, within Shewana3_0349 at 383.807 kb on - strand, within Shewana3_0349 at 383.844 kb on + strand, within Shewana3_0349 at 383.955 kb on - strand, within Shewana3_0349 at 383.991 kb on - strand, within Shewana3_0349 at 384.102 kb on - strand, within Shewana3_0349 at 384.169 kb on - strand, within Shewana3_0349 at 384.239 kb on - strand at 384.252 kb on - strand at 384.400 kb on - strand at 384.435 kb on + strand at 384.447 kb on + strand at 384.487 kb on + strand at 384.614 kb on + strand, within Shewana3_0350 at 384.614 kb on + strand, within Shewana3_0350 at 384.614 kb on + strand, within Shewana3_0350 at 384.614 kb on + strand, within Shewana3_0350 at 384.614 kb on + strand, within Shewana3_0350 at 384.622 kb on - strand, within Shewana3_0350 at 384.622 kb on - strand, within Shewana3_0350 at 384.622 kb on - strand, within Shewana3_0350 at 384.689 kb on + strand, within Shewana3_0350 at 384.694 kb on + strand, within Shewana3_0350 at 384.694 kb on + strand, within Shewana3_0350 at 384.694 kb on + strand, within Shewana3_0350 at 384.709 kb on + strand, within Shewana3_0350 at 384.717 kb on - strand, within Shewana3_0350 at 384.717 kb on - strand, within Shewana3_0350 at 384.717 kb on - strand, within Shewana3_0350 at 384.794 kb on + strand, within Shewana3_0350 at 384.901 kb on + strand, within Shewana3_0350 at 384.971 kb on + strand, within Shewana3_0350 at 384.983 kb on - strand, within Shewana3_0350 at 385.088 kb on + strand, within Shewana3_0350 at 385.095 kb on + strand, within Shewana3_0350 at 385.209 kb on - strand, within Shewana3_0350 at 385.334 kb on - strand, within Shewana3_0350 at 385.401 kb on + strand, within Shewana3_0350 at 385.546 kb on + strand, within Shewana3_0350 at 385.554 kb on - strand, within Shewana3_0350 at 385.647 kb on - strand, within Shewana3_0350 at 385.775 kb on + strand, within Shewana3_0350 at 385.783 kb on - strand, within Shewana3_0350 at 385.831 kb on - strand, within Shewana3_0350 at 385.831 kb on - strand, within Shewana3_0350 at 385.841 kb on + strand, within Shewana3_0350 at 385.895 kb on + strand, within Shewana3_0350 at 385.903 kb on - strand, within Shewana3_0350 at 385.924 kb on - strand, within Shewana3_0350 at 386.041 kb on + strand at 386.050 kb on + strand at 386.050 kb on + strand at 386.059 kb on - strand at 386.113 kb on - strand at 386.118 kb on - strand at 386.276 kb on + strand at 386.284 kb on - strand at 386.368 kb on + strand, within Shewana3_0351 at 386.373 kb on + strand, within Shewana3_0351 at 386.436 kb on - strand, within Shewana3_0351 at 386.624 kb on + strand, within Shewana3_0351 at 386.624 kb on + strand, within Shewana3_0351 at 386.759 kb on - strand, within Shewana3_0352 at 386.830 kb on + strand, within Shewana3_0352 at 386.887 kb on + strand, within Shewana3_0352 at 386.895 kb on - strand, within Shewana3_0352 at 386.895 kb on - strand, within Shewana3_0352 at 386.895 kb on - strand, within Shewana3_0352 at 387.045 kb on + strand, within Shewana3_0352
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 383,335 + -0.5 383,383 + Shewana3_0349 0.13 -1.5 383,383 + Shewana3_0349 0.13 +0.6 383,391 - Shewana3_0349 0.13 +0.8 383,406 - Shewana3_0349 0.15 -2.0 383,580 + Shewana3_0349 0.31 -0.6 383,587 - Shewana3_0349 0.32 -0.3 383,587 - Shewana3_0349 0.32 -1.5 383,605 + Shewana3_0349 0.34 +0.1 383,701 - Shewana3_0349 0.43 -1.4 383,741 + Shewana3_0349 0.47 +0.2 383,749 - Shewana3_0349 0.47 -0.7 383,788 + Shewana3_0349 0.51 +0.7 383,796 - Shewana3_0349 0.52 +0.1 383,799 + Shewana3_0349 0.52 +0.2 383,807 - Shewana3_0349 0.53 -1.2 383,807 - Shewana3_0349 0.53 +0.9 383,844 + Shewana3_0349 0.57 -0.1 383,955 - Shewana3_0349 0.67 +0.1 383,991 - Shewana3_0349 0.71 -3.0 384,102 - Shewana3_0349 0.81 -0.9 384,169 - Shewana3_0349 0.88 -0.7 384,239 - -0.5 384,252 - -0.2 384,400 - -0.1 384,435 + -0.4 384,447 + +0.6 384,487 + +0.8 384,614 + Shewana3_0350 0.16 +0.0 384,614 + Shewana3_0350 0.16 -0.5 384,614 + Shewana3_0350 0.16 +0.0 384,614 + Shewana3_0350 0.16 -1.3 384,614 + Shewana3_0350 0.16 -0.9 384,622 - Shewana3_0350 0.17 -0.3 384,622 - Shewana3_0350 0.17 -1.3 384,622 - Shewana3_0350 0.17 -1.1 384,689 + Shewana3_0350 0.21 -0.3 384,694 + Shewana3_0350 0.21 -0.6 384,694 + Shewana3_0350 0.21 +1.0 384,694 + Shewana3_0350 0.21 +2.0 384,709 + Shewana3_0350 0.22 -1.1 384,717 - Shewana3_0350 0.22 -0.8 384,717 - Shewana3_0350 0.22 -1.3 384,717 - Shewana3_0350 0.22 -1.5 384,794 + Shewana3_0350 0.26 -1.5 384,901 + Shewana3_0350 0.32 +0.1 384,971 + Shewana3_0350 0.36 -0.5 384,983 - Shewana3_0350 0.37 +0.6 385,088 + Shewana3_0350 0.43 +0.2 385,095 + Shewana3_0350 0.43 +0.2 385,209 - Shewana3_0350 0.49 +1.2 385,334 - Shewana3_0350 0.56 -0.5 385,401 + Shewana3_0350 0.60 -0.3 385,546 + Shewana3_0350 0.68 -0.4 385,554 - Shewana3_0350 0.68 +0.2 385,647 - Shewana3_0350 0.73 +0.2 385,775 + Shewana3_0350 0.80 -1.0 385,783 - Shewana3_0350 0.81 +0.6 385,831 - Shewana3_0350 0.83 -1.0 385,831 - Shewana3_0350 0.83 +0.5 385,841 + Shewana3_0350 0.84 -0.1 385,895 + Shewana3_0350 0.87 -1.0 385,903 - Shewana3_0350 0.87 -0.6 385,924 - Shewana3_0350 0.89 -0.3 386,041 + -0.6 386,050 + +0.3 386,050 + +0.3 386,059 - +0.4 386,113 - -1.5 386,118 - -1.1 386,276 + +1.1 386,284 - -0.4 386,368 + Shewana3_0351 0.20 -0.7 386,373 + Shewana3_0351 0.21 -0.3 386,436 - Shewana3_0351 0.37 -0.2 386,624 + Shewana3_0351 0.82 -0.9 386,624 + Shewana3_0351 0.82 +0.2 386,759 - Shewana3_0352 0.12 -0.8 386,830 + Shewana3_0352 0.20 +0.3 386,887 + Shewana3_0352 0.27 +0.3 386,895 - Shewana3_0352 0.28 -1.1 386,895 - Shewana3_0352 0.28 -0.1 386,895 - Shewana3_0352 0.28 -0.2 387,045 + Shewana3_0352 0.46 -1.3
Or see this region's nucleotide sequence