Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0341 and Shewana3_0342 are separated by 6 nucleotides Shewana3_0342 and Shewana3_0343 overlap by 1 nucleotides Shewana3_0343 and Shewana3_0344 are separated by 9 nucleotides Shewana3_0344 and Shewana3_0345 overlap by 14 nucleotides Shewana3_0345 and Shewana3_0346 overlap by 8 nucleotides
Shewana3_0341: Shewana3_0341 - integrase catalytic subunit (RefSeq), at 376,047 to 376,850
_0341
Shewana3_0342: Shewana3_0342 - hypothetical protein (RefSeq), at 376,857 to 377,240
_0342
Shewana3_0343: Shewana3_0343 - acyl carrier protein (RefSeq), at 377,240 to 377,488
_0343
Shewana3_0344: Shewana3_0344 - acyl carrier protein, putative (RefSeq), at 377,498 to 377,752
_0344
Shewana3_0345: Shewana3_0345 - phospholipid/glycerol acyltransferase (RefSeq), at 377,739 to 378,587
_0345
Shewana3_0346: Shewana3_0346 - hypothetical protein (RefSeq), at 378,580 to 379,314
_0346
Position (kb) 377
378
379 Strain fitness (log2 ratio) -2
-1
0
1
2 at 376.875 kb on + strand at 376.943 kb on + strand, within Shewana3_0342 at 377.058 kb on - strand, within Shewana3_0342 at 377.059 kb on - strand, within Shewana3_0342 at 377.113 kb on - strand, within Shewana3_0342 at 377.144 kb on - strand, within Shewana3_0342 at 377.149 kb on - strand, within Shewana3_0342 at 377.243 kb on + strand at 377.265 kb on + strand, within Shewana3_0343 at 377.362 kb on + strand, within Shewana3_0343 at 377.362 kb on + strand, within Shewana3_0343 at 377.372 kb on + strand, within Shewana3_0343 at 377.380 kb on - strand, within Shewana3_0343 at 377.635 kb on + strand, within Shewana3_0344 at 377.683 kb on + strand, within Shewana3_0344 at 378.078 kb on + strand, within Shewana3_0345 at 378.086 kb on - strand, within Shewana3_0345 at 378.113 kb on - strand, within Shewana3_0345 at 378.193 kb on - strand, within Shewana3_0345 at 378.295 kb on + strand, within Shewana3_0345 at 378.350 kb on - strand, within Shewana3_0345 at 378.390 kb on - strand, within Shewana3_0345 at 378.410 kb on + strand, within Shewana3_0345 at 378.539 kb on + strand at 378.547 kb on - strand at 378.567 kb on + strand at 378.624 kb on + strand at 378.675 kb on - strand, within Shewana3_0346 at 378.698 kb on + strand, within Shewana3_0346 at 378.728 kb on - strand, within Shewana3_0346 at 378.774 kb on - strand, within Shewana3_0346 at 379.038 kb on + strand, within Shewana3_0346 at 379.046 kb on - strand, within Shewana3_0346 at 379.090 kb on + strand, within Shewana3_0346 at 379.169 kb on - strand, within Shewana3_0346 at 379.190 kb on - strand, within Shewana3_0346 at 379.190 kb on - strand, within Shewana3_0346 at 379.332 kb on + strand at 379.340 kb on - strand at 379.418 kb on - strand at 379.467 kb on + strand at 379.583 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 376,875 + -1.0 376,943 + Shewana3_0342 0.22 -0.4 377,058 - Shewana3_0342 0.52 +0.5 377,059 - Shewana3_0342 0.53 -1.1 377,113 - Shewana3_0342 0.67 -0.4 377,144 - Shewana3_0342 0.75 +0.4 377,149 - Shewana3_0342 0.76 +0.1 377,243 + -1.5 377,265 + Shewana3_0343 0.10 -0.5 377,362 + Shewana3_0343 0.49 -0.9 377,362 + Shewana3_0343 0.49 -0.8 377,372 + Shewana3_0343 0.53 -0.5 377,380 - Shewana3_0343 0.56 +0.7 377,635 + Shewana3_0344 0.54 -1.5 377,683 + Shewana3_0344 0.73 -1.3 378,078 + Shewana3_0345 0.40 -0.1 378,086 - Shewana3_0345 0.41 +0.3 378,113 - Shewana3_0345 0.44 -0.3 378,193 - Shewana3_0345 0.53 -0.2 378,295 + Shewana3_0345 0.65 -0.3 378,350 - Shewana3_0345 0.72 +0.2 378,390 - Shewana3_0345 0.77 -0.9 378,410 + Shewana3_0345 0.79 +0.1 378,539 + +1.8 378,547 - +0.3 378,567 + -2.3 378,624 + -0.2 378,675 - Shewana3_0346 0.13 +0.0 378,698 + Shewana3_0346 0.16 -0.3 378,728 - Shewana3_0346 0.20 -0.2 378,774 - Shewana3_0346 0.26 -0.4 379,038 + Shewana3_0346 0.62 -0.1 379,046 - Shewana3_0346 0.63 -0.2 379,090 + Shewana3_0346 0.69 -0.2 379,169 - Shewana3_0346 0.80 -0.7 379,190 - Shewana3_0346 0.83 -0.2 379,190 - Shewana3_0346 0.83 -0.9 379,332 + -1.3 379,340 - -0.8 379,418 - -0.1 379,467 + -0.2 379,583 + +1.0
Or see this region's nucleotide sequence