Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0325 and Shewana3_0326 are separated by 10 nucleotides Shewana3_0326 and fabG are separated by 0 nucleotides fabG and Shewana3_0328 are separated by 125 nucleotides
Shewana3_0325: Shewana3_0325 - hypothetical protein (RefSeq), at 359,707 to 360,450
_0325
Shewana3_0326: Shewana3_0326 - 3-oxoacyl-(acyl carrier protein) synthase II (RefSeq), at 360,461 to 361,714
_0326
Shewana3_0327: fabG - 3-ketoacyl-(acyl-carrier-protein) reductase (RefSeq), at 361,715 to 362,440
fabG
Shewana3_0328: Shewana3_0328 - thioester dehydrase family protein (RefSeq), at 362,566 to 363,105
_0328
Position (kb) 360
361
362 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 359.489 kb on + strand at 359.497 kb on - strand at 359.532 kb on - strand at 359.550 kb on + strand at 359.556 kb on + strand at 359.571 kb on + strand at 359.579 kb on - strand at 359.579 kb on - strand at 359.579 kb on - strand at 359.611 kb on - strand at 359.616 kb on - strand at 359.618 kb on + strand at 359.633 kb on - strand at 359.633 kb on - strand at 359.638 kb on + strand at 359.705 kb on + strand at 359.713 kb on - strand at 359.829 kb on - strand, within Shewana3_0325 at 359.866 kb on + strand, within Shewana3_0325 at 360.181 kb on + strand, within Shewana3_0325 at 360.218 kb on - strand, within Shewana3_0325 at 360.248 kb on - strand, within Shewana3_0325 at 360.312 kb on - strand, within Shewana3_0325 at 360.312 kb on - strand, within Shewana3_0325 at 360.375 kb on - strand, within Shewana3_0325 at 360.406 kb on - strand at 360.411 kb on + strand at 360.651 kb on - strand, within Shewana3_0326 at 360.686 kb on + strand, within Shewana3_0326 at 360.709 kb on - strand, within Shewana3_0326 at 360.763 kb on - strand, within Shewana3_0326 at 360.969 kb on - strand, within Shewana3_0326 at 361.180 kb on - strand, within Shewana3_0326 at 361.268 kb on + strand, within Shewana3_0326 at 361.286 kb on + strand, within Shewana3_0326 at 361.302 kb on - strand, within Shewana3_0326 at 361.362 kb on - strand, within Shewana3_0326 at 361.404 kb on - strand, within Shewana3_0326 at 361.612 kb on + strand at 361.612 kb on + strand at 361.612 kb on + strand at 361.612 kb on + strand at 361.620 kb on - strand at 361.620 kb on - strand at 361.620 kb on - strand at 361.620 kb on - strand at 361.654 kb on - strand at 361.691 kb on + strand at 361.699 kb on - strand at 361.838 kb on - strand, within fabG at 361.845 kb on + strand, within fabG at 361.845 kb on + strand, within fabG at 361.853 kb on - strand, within fabG at 361.930 kb on + strand, within fabG at 361.930 kb on + strand, within fabG at 361.930 kb on + strand, within fabG at 361.930 kb on + strand, within fabG at 361.938 kb on - strand, within fabG at 361.938 kb on - strand, within fabG at 361.938 kb on - strand, within fabG at 361.938 kb on - strand, within fabG at 361.957 kb on + strand, within fabG at 361.968 kb on + strand, within fabG at 361.993 kb on + strand, within fabG at 362.001 kb on - strand, within fabG at 362.001 kb on - strand, within fabG at 362.020 kb on - strand, within fabG at 362.191 kb on - strand, within fabG at 362.260 kb on + strand, within fabG at 362.302 kb on + strand, within fabG at 362.303 kb on - strand, within fabG at 362.310 kb on - strand, within fabG at 362.420 kb on + strand at 362.501 kb on + strand at 362.529 kb on + strand at 362.529 kb on + strand at 362.529 kb on + strand at 362.537 kb on - strand at 362.564 kb on + strand at 362.564 kb on + strand at 362.564 kb on + strand at 362.572 kb on - strand at 362.712 kb on - strand, within Shewana3_0328
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 359,489 + +0.7 359,497 - -3.4 359,532 - -0.5 359,550 + +0.0 359,556 + +0.1 359,571 + +0.3 359,579 - -1.5 359,579 - -1.5 359,579 - -1.0 359,611 - -0.8 359,616 - -0.8 359,618 + +0.3 359,633 - -1.0 359,633 - -0.8 359,638 + +0.4 359,705 + +0.2 359,713 - +0.1 359,829 - Shewana3_0325 0.16 +1.0 359,866 + Shewana3_0325 0.21 -1.2 360,181 + Shewana3_0325 0.64 -0.8 360,218 - Shewana3_0325 0.69 -1.1 360,248 - Shewana3_0325 0.73 -0.9 360,312 - Shewana3_0325 0.81 -0.5 360,312 - Shewana3_0325 0.81 -0.6 360,375 - Shewana3_0325 0.90 +0.4 360,406 - -1.0 360,411 + +0.1 360,651 - Shewana3_0326 0.15 -0.1 360,686 + Shewana3_0326 0.18 +0.5 360,709 - Shewana3_0326 0.20 +0.3 360,763 - Shewana3_0326 0.24 +0.2 360,969 - Shewana3_0326 0.41 -0.3 361,180 - Shewana3_0326 0.57 +0.2 361,268 + Shewana3_0326 0.64 -0.5 361,286 + Shewana3_0326 0.66 -1.6 361,302 - Shewana3_0326 0.67 +0.2 361,362 - Shewana3_0326 0.72 +0.0 361,404 - Shewana3_0326 0.75 -0.3 361,612 + -0.5 361,612 + -0.8 361,612 + -0.4 361,612 + -0.3 361,620 - -0.8 361,620 - +0.1 361,620 - -0.0 361,620 - +1.1 361,654 - -0.1 361,691 + -1.1 361,699 - +0.9 361,838 - fabG Shewana3_0327 0.17 -0.2 361,845 + fabG Shewana3_0327 0.18 -1.7 361,845 + fabG Shewana3_0327 0.18 -0.5 361,853 - fabG Shewana3_0327 0.19 -0.1 361,930 + fabG Shewana3_0327 0.30 +0.2 361,930 + fabG Shewana3_0327 0.30 -0.0 361,930 + fabG Shewana3_0327 0.30 -0.1 361,930 + fabG Shewana3_0327 0.30 +0.4 361,938 - fabG Shewana3_0327 0.31 -0.6 361,938 - fabG Shewana3_0327 0.31 -0.7 361,938 - fabG Shewana3_0327 0.31 +0.0 361,938 - fabG Shewana3_0327 0.31 -0.3 361,957 + fabG Shewana3_0327 0.33 +0.8 361,968 + fabG Shewana3_0327 0.35 -1.4 361,993 + fabG Shewana3_0327 0.38 -0.5 362,001 - fabG Shewana3_0327 0.39 -0.4 362,001 - fabG Shewana3_0327 0.39 -0.6 362,020 - fabG Shewana3_0327 0.42 -0.1 362,191 - fabG Shewana3_0327 0.66 +0.9 362,260 + fabG Shewana3_0327 0.75 -1.2 362,302 + fabG Shewana3_0327 0.81 -0.8 362,303 - fabG Shewana3_0327 0.81 -0.4 362,310 - fabG Shewana3_0327 0.82 +0.5 362,420 + -0.5 362,501 + -0.2 362,529 + -0.9 362,529 + +0.8 362,529 + +0.6 362,537 - +0.3 362,564 + +0.8 362,564 + +0.1 362,564 + +0.6 362,572 - -0.1 362,712 - Shewana3_0328 0.27 +0.2
Or see this region's nucleotide sequence