Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0315 and Shewana3_0316 are separated by 133 nucleotides Shewana3_0316 and Shewana3_0317 are separated by 61 nucleotides Shewana3_0317 and Shewana3_0318 are separated by 41 nucleotides Shewana3_0318 and Shewana3_0319 are separated by 95 nucleotides Shewana3_0319 and Shewana3_0320 are separated by 126 nucleotides
Shewana3_0315: Shewana3_0315 - thioesterase domain-containing protein (RefSeq), at 352,381 to 352,818
_0315
Shewana3_0316: Shewana3_0316 - azoreductase (RefSeq), at 352,952 to 353,548
_0316
Shewana3_0317: Shewana3_0317 - hypothetical protein (RefSeq), at 353,610 to 353,774
_0317
Shewana3_0318: Shewana3_0318 - rhodanese domain-containing protein (RefSeq), at 353,816 to 354,139
_0318
Shewana3_0319: Shewana3_0319 - XRE family transcriptional regulator (RefSeq), at 354,235 to 354,810
_0319
Shewana3_0320: Shewana3_0320 - benzoate transporter (RefSeq), at 354,937 to 356,127
_0320
Position (kb) 353
354
355 Strain fitness (log2 ratio) -2
-1
0
1 at 352.909 kb on - strand at 353.023 kb on + strand, within Shewana3_0316 at 353.115 kb on + strand, within Shewana3_0316 at 353.173 kb on + strand, within Shewana3_0316 at 353.202 kb on - strand, within Shewana3_0316 at 353.291 kb on + strand, within Shewana3_0316 at 353.351 kb on + strand, within Shewana3_0316 at 353.359 kb on - strand, within Shewana3_0316 at 353.359 kb on - strand, within Shewana3_0316 at 353.378 kb on + strand, within Shewana3_0316 at 353.438 kb on - strand, within Shewana3_0316 at 353.629 kb on - strand, within Shewana3_0317 at 353.636 kb on + strand, within Shewana3_0317 at 353.719 kb on + strand, within Shewana3_0317 at 353.727 kb on - strand, within Shewana3_0317 at 353.807 kb on + strand at 353.877 kb on + strand, within Shewana3_0318 at 353.943 kb on - strand, within Shewana3_0318 at 354.137 kb on - strand at 354.220 kb on + strand at 354.354 kb on + strand, within Shewana3_0319 at 354.362 kb on - strand, within Shewana3_0319 at 354.426 kb on + strand, within Shewana3_0319 at 354.657 kb on + strand, within Shewana3_0319 at 354.665 kb on - strand, within Shewana3_0319 at 354.680 kb on - strand, within Shewana3_0319 at 354.712 kb on + strand, within Shewana3_0319 at 354.745 kb on + strand, within Shewana3_0319 at 354.753 kb on + strand at 355.093 kb on - strand, within Shewana3_0320 at 355.097 kb on + strand, within Shewana3_0320
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 352,909 - +0.0 353,023 + Shewana3_0316 0.12 +0.3 353,115 + Shewana3_0316 0.27 +0.4 353,173 + Shewana3_0316 0.37 +0.7 353,202 - Shewana3_0316 0.42 -1.1 353,291 + Shewana3_0316 0.57 +0.6 353,351 + Shewana3_0316 0.67 +0.7 353,359 - Shewana3_0316 0.68 -1.5 353,359 - Shewana3_0316 0.68 -1.5 353,378 + Shewana3_0316 0.71 +1.1 353,438 - Shewana3_0316 0.81 -0.3 353,629 - Shewana3_0317 0.12 +0.7 353,636 + Shewana3_0317 0.16 -1.5 353,719 + Shewana3_0317 0.66 -0.3 353,727 - Shewana3_0317 0.71 +0.4 353,807 + +0.5 353,877 + Shewana3_0318 0.19 +0.3 353,943 - Shewana3_0318 0.39 +0.2 354,137 - +0.5 354,220 + +0.0 354,354 + Shewana3_0319 0.21 -0.4 354,362 - Shewana3_0319 0.22 -0.6 354,426 + Shewana3_0319 0.33 -2.0 354,657 + Shewana3_0319 0.73 +0.1 354,665 - Shewana3_0319 0.75 -1.0 354,680 - Shewana3_0319 0.77 -2.3 354,712 + Shewana3_0319 0.83 -0.3 354,745 + Shewana3_0319 0.89 -1.9 354,753 + -0.5 355,093 - Shewana3_0320 0.13 -1.1 355,097 + Shewana3_0320 0.13 +0.3
Or see this region's nucleotide sequence