Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0280 and Shewana3_0281 are separated by 325 nucleotides Shewana3_0281 and Shewana3_0282 are separated by 46 nucleotides Shewana3_0282 and Shewana3_0283 are separated by 70 nucleotides
Shewana3_0280: Shewana3_0280 - ABC transporter related (RefSeq), at 313,623 to 314,726
_0280
Shewana3_0281: Shewana3_0281 - hypothetical protein (RefSeq), at 315,052 to 315,573
_0281
Shewana3_0282: Shewana3_0282 - two component transcriptional regulator (RefSeq), at 315,620 to 316,297
_0282
Shewana3_0283: Shewana3_0283 - ATPase domain-containing protein (RefSeq), at 316,368 to 317,603
_0283
Position (kb) 315
316 Strain fitness (log2 ratio) -2
-1
0
1 at 314.181 kb on + strand, within Shewana3_0280 at 314.372 kb on - strand, within Shewana3_0280 at 314.568 kb on - strand, within Shewana3_0280 at 314.666 kb on + strand at 314.959 kb on + strand at 315.063 kb on - strand at 315.149 kb on + strand, within Shewana3_0281 at 315.377 kb on + strand, within Shewana3_0281 at 315.568 kb on + strand at 315.576 kb on - strand at 315.576 kb on - strand at 315.576 kb on - strand at 315.576 kb on - strand at 315.600 kb on - strand at 315.605 kb on - strand at 315.768 kb on - strand, within Shewana3_0282 at 315.850 kb on - strand, within Shewana3_0282 at 315.925 kb on - strand, within Shewana3_0282 at 315.981 kb on - strand, within Shewana3_0282 at 315.999 kb on + strand, within Shewana3_0282 at 316.035 kb on + strand, within Shewana3_0282 at 316.070 kb on - strand, within Shewana3_0282 at 316.257 kb on - strand at 316.299 kb on - strand at 316.300 kb on - strand at 316.543 kb on - strand, within Shewana3_0283 at 316.560 kb on + strand, within Shewana3_0283 at 316.560 kb on + strand, within Shewana3_0283 at 316.560 kb on + strand, within Shewana3_0283
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 314,181 + Shewana3_0280 0.51 -0.3 314,372 - Shewana3_0280 0.68 -0.6 314,568 - Shewana3_0280 0.86 -0.3 314,666 + +0.2 314,959 + -0.3 315,063 - -1.4 315,149 + Shewana3_0281 0.19 +1.0 315,377 + Shewana3_0281 0.62 +1.1 315,568 + -0.1 315,576 - -1.4 315,576 - -2.3 315,576 - -2.6 315,576 - -0.9 315,600 - +0.2 315,605 - -1.3 315,768 - Shewana3_0282 0.22 +1.7 315,850 - Shewana3_0282 0.34 -0.8 315,925 - Shewana3_0282 0.45 -1.1 315,981 - Shewana3_0282 0.53 +0.2 315,999 + Shewana3_0282 0.56 -0.1 316,035 + Shewana3_0282 0.61 -0.4 316,070 - Shewana3_0282 0.66 -0.0 316,257 - -1.2 316,299 - -1.3 316,300 - -0.6 316,543 - Shewana3_0283 0.14 -1.0 316,560 + Shewana3_0283 0.16 +0.4 316,560 + Shewana3_0283 0.16 +0.2 316,560 + Shewana3_0283 0.16 +0.5
Or see this region's nucleotide sequence