Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt moaA and moaC are separated by 112 nucleotides moaC and Shewana3_0276 are separated by 19 nucleotides Shewana3_0276 and Shewana3_0277 are separated by 1 nucleotides Shewana3_0277 and Shewana3_0278 are separated by 39 nucleotides
Shewana3_0274: moaA - molybdenum cofactor biosynthesis protein A (RefSeq), at 309,765 to 310,778
moaA
Shewana3_0275: moaC - molybdenum cofactor biosynthesis protein C (RefSeq), at 310,891 to 311,370
moaC
Shewana3_0276: Shewana3_0276 - molybdopterin synthase subunit MoaD (RefSeq), at 311,390 to 311,641
_0276
Shewana3_0277: Shewana3_0277 - molybdopterin synthase subunit MoaE (RefSeq), at 311,643 to 312,110
_0277
Shewana3_0278: Shewana3_0278 - molybdenum ABC transporter, periplasmic molybdate-binding protein (RefSeq), at 312,150 to 312,953
_0278
Position (kb) 311
312 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 310.402 kb on - strand, within moaA at 310.496 kb on - strand, within moaA at 310.554 kb on - strand, within moaA at 310.573 kb on - strand, within moaA at 310.709 kb on + strand at 310.893 kb on - strand at 310.931 kb on - strand at 311.113 kb on + strand, within moaC at 311.189 kb on + strand, within moaC at 311.189 kb on + strand, within moaC at 311.189 kb on + strand, within moaC at 311.189 kb on + strand, within moaC at 311.197 kb on - strand, within moaC at 311.197 kb on - strand, within moaC at 311.197 kb on - strand, within moaC at 311.197 kb on - strand, within moaC at 311.280 kb on + strand, within moaC at 311.280 kb on + strand, within moaC at 311.280 kb on + strand, within moaC at 311.280 kb on + strand, within moaC at 311.280 kb on + strand, within moaC at 311.280 kb on + strand, within moaC at 311.280 kb on + strand, within moaC at 311.280 kb on + strand, within moaC at 311.280 kb on + strand, within moaC at 311.288 kb on - strand, within moaC at 311.288 kb on - strand, within moaC at 311.288 kb on - strand, within moaC at 311.288 kb on - strand, within moaC at 311.355 kb on + strand at 311.355 kb on + strand at 311.363 kb on - strand at 311.528 kb on + strand, within Shewana3_0276 at 311.680 kb on + strand at 311.784 kb on - strand, within Shewana3_0277 at 311.798 kb on - strand, within Shewana3_0277 at 311.805 kb on - strand, within Shewana3_0277 at 311.821 kb on - strand, within Shewana3_0277 at 311.881 kb on - strand, within Shewana3_0277 at 311.950 kb on - strand, within Shewana3_0277 at 312.041 kb on + strand, within Shewana3_0277 at 312.105 kb on + strand at 312.214 kb on + strand at 312.222 kb on - strand at 312.252 kb on - strand, within Shewana3_0278 at 312.283 kb on + strand, within Shewana3_0278 at 312.519 kb on + strand, within Shewana3_0278 at 312.527 kb on - strand, within Shewana3_0278 at 312.606 kb on - strand, within Shewana3_0278 at 312.606 kb on - strand, within Shewana3_0278
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 310,402 - moaA Shewana3_0274 0.63 -0.2 310,496 - moaA Shewana3_0274 0.72 -0.4 310,554 - moaA Shewana3_0274 0.78 -0.7 310,573 - moaA Shewana3_0274 0.80 -0.1 310,709 + +2.4 310,893 - -1.0 310,931 - -2.2 311,113 + moaC Shewana3_0275 0.46 +1.1 311,189 + moaC Shewana3_0275 0.62 -2.5 311,189 + moaC Shewana3_0275 0.62 -0.5 311,189 + moaC Shewana3_0275 0.62 -2.2 311,189 + moaC Shewana3_0275 0.62 -1.0 311,197 - moaC Shewana3_0275 0.64 -2.3 311,197 - moaC Shewana3_0275 0.64 -3.3 311,197 - moaC Shewana3_0275 0.64 -2.1 311,197 - moaC Shewana3_0275 0.64 -2.2 311,280 + moaC Shewana3_0275 0.81 -0.1 311,280 + moaC Shewana3_0275 0.81 -1.4 311,280 + moaC Shewana3_0275 0.81 +0.3 311,280 + moaC Shewana3_0275 0.81 -1.4 311,280 + moaC Shewana3_0275 0.81 -1.4 311,280 + moaC Shewana3_0275 0.81 +0.6 311,280 + moaC Shewana3_0275 0.81 -0.9 311,280 + moaC Shewana3_0275 0.81 +0.1 311,280 + moaC Shewana3_0275 0.81 -0.9 311,288 - moaC Shewana3_0275 0.83 -1.8 311,288 - moaC Shewana3_0275 0.83 -1.5 311,288 - moaC Shewana3_0275 0.83 -2.3 311,288 - moaC Shewana3_0275 0.83 -2.3 311,355 + +0.6 311,355 + -0.1 311,363 - -1.0 311,528 + Shewana3_0276 0.55 +1.1 311,680 + +0.7 311,784 - Shewana3_0277 0.30 -0.3 311,798 - Shewana3_0277 0.33 -0.1 311,805 - Shewana3_0277 0.35 -2.1 311,821 - Shewana3_0277 0.38 -1.4 311,881 - Shewana3_0277 0.51 -0.6 311,950 - Shewana3_0277 0.66 -1.1 312,041 + Shewana3_0277 0.85 +0.6 312,105 + -0.4 312,214 + +0.2 312,222 - -1.5 312,252 - Shewana3_0278 0.13 -2.1 312,283 + Shewana3_0278 0.17 -0.5 312,519 + Shewana3_0278 0.46 +0.1 312,527 - Shewana3_0278 0.47 -0.1 312,606 - Shewana3_0278 0.57 -2.4 312,606 - Shewana3_0278 0.57 -1.0
Or see this region's nucleotide sequence