Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0253 and Shewana3_0254 are separated by 302 nucleotides Shewana3_0254 and Shewana3_0255 are separated by 50 nucleotides Shewana3_0255 and Shewana3_0256 are separated by 38 nucleotides
Shewana3_0253: Shewana3_0253 - two component transcriptional regulator (RefSeq), at 285,338 to 286,024
_0253
Shewana3_0254: Shewana3_0254 - protein of unknown function, Spy-related (RefSeq), at 286,327 to 286,818
_0254
Shewana3_0255: Shewana3_0255 - cation diffusion facilitator family transporter (RefSeq), at 286,869 to 287,768
_0255
Shewana3_0256: Shewana3_0256 - hypothetical protein (RefSeq), at 287,807 to 288,217
_0256
Position (kb) 286
287 Strain fitness (log2 ratio) -2
-1
0
1 at 285.498 kb on - strand, within Shewana3_0253 at 285.794 kb on + strand, within Shewana3_0253 at 285.863 kb on + strand, within Shewana3_0253 at 285.907 kb on + strand, within Shewana3_0253 at 285.907 kb on + strand, within Shewana3_0253 at 285.907 kb on + strand, within Shewana3_0253 at 285.915 kb on - strand, within Shewana3_0253 at 285.915 kb on - strand, within Shewana3_0253 at 285.915 kb on - strand, within Shewana3_0253 at 285.915 kb on - strand, within Shewana3_0253 at 285.927 kb on + strand, within Shewana3_0253 at 285.943 kb on + strand, within Shewana3_0253 at 286.049 kb on + strand at 286.058 kb on + strand at 286.125 kb on + strand at 286.195 kb on - strand at 286.226 kb on - strand at 286.282 kb on - strand at 286.286 kb on + strand at 286.434 kb on + strand, within Shewana3_0254 at 286.676 kb on + strand, within Shewana3_0254 at 286.751 kb on + strand, within Shewana3_0254 at 286.751 kb on + strand, within Shewana3_0254 at 286.812 kb on + strand at 286.850 kb on - strand at 286.947 kb on - strand at 286.967 kb on + strand, within Shewana3_0255 at 287.034 kb on + strand, within Shewana3_0255 at 287.107 kb on - strand, within Shewana3_0255 at 287.486 kb on - strand, within Shewana3_0255 at 287.494 kb on + strand, within Shewana3_0255 at 287.502 kb on - strand, within Shewana3_0255 at 287.589 kb on - strand, within Shewana3_0255 at 287.599 kb on - strand, within Shewana3_0255 at 287.719 kb on - strand at 287.744 kb on + strand at 287.744 kb on + strand at 287.809 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 285,498 - Shewana3_0253 0.23 -0.9 285,794 + Shewana3_0253 0.66 +0.4 285,863 + Shewana3_0253 0.76 -2.0 285,907 + Shewana3_0253 0.83 -0.9 285,907 + Shewana3_0253 0.83 -0.6 285,907 + Shewana3_0253 0.83 +0.1 285,915 - Shewana3_0253 0.84 -0.5 285,915 - Shewana3_0253 0.84 +1.4 285,915 - Shewana3_0253 0.84 -1.2 285,915 - Shewana3_0253 0.84 -0.4 285,927 + Shewana3_0253 0.86 -1.7 285,943 + Shewana3_0253 0.88 -1.6 286,049 + +0.6 286,058 + -0.3 286,125 + -0.3 286,195 - -0.4 286,226 - +0.0 286,282 - -0.1 286,286 + -0.2 286,434 + Shewana3_0254 0.22 -0.5 286,676 + Shewana3_0254 0.71 +1.3 286,751 + Shewana3_0254 0.86 +0.7 286,751 + Shewana3_0254 0.86 +0.1 286,812 + -0.4 286,850 - -0.4 286,947 - -0.7 286,967 + Shewana3_0255 0.11 +0.2 287,034 + Shewana3_0255 0.18 -1.3 287,107 - Shewana3_0255 0.26 -0.4 287,486 - Shewana3_0255 0.69 +0.2 287,494 + Shewana3_0255 0.69 -0.4 287,502 - Shewana3_0255 0.70 +0.2 287,589 - Shewana3_0255 0.80 -1.0 287,599 - Shewana3_0255 0.81 +0.4 287,719 - -0.6 287,744 + -0.3 287,744 + +1.2 287,809 + +1.1
Or see this region's nucleotide sequence