Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0243 and Shewana3_0244 are separated by 47 nucleotides Shewana3_0244 and Shewana3_0245 are separated by 33 nucleotides Shewana3_0245 and Shewana3_0246 are separated by 206 nucleotides Shewana3_0246 and Shewana3_0247 are separated by 28 nucleotides
Shewana3_0243: Shewana3_0243 - diheme cytochrome c (RefSeq), at 275,464 to 276,027
_0243
Shewana3_0244: Shewana3_0244 - cytochrome c-type protein Shp (RefSeq), at 276,075 to 276,509
_0244
Shewana3_0245: Shewana3_0245 - cytochrome B561 (RefSeq), at 276,543 to 277,139
_0245
Shewana3_0246: Shewana3_0246 - hypothetical protein (RefSeq), at 277,346 to 277,768
_0246
Shewana3_0247: Shewana3_0247 - hypothetical protein (RefSeq), at 277,797 to 278,285
_0247
Position (kb)
276
277
278 Strain fitness (log2 ratio)
-1
0
1 at 275.814 kb on - strand, within Shewana3_0243 at 275.846 kb on - strand, within Shewana3_0243 at 275.990 kb on - strand at 276.095 kb on + strand at 276.158 kb on - strand, within Shewana3_0244 at 276.186 kb on + strand, within Shewana3_0244 at 276.308 kb on + strand, within Shewana3_0244 at 276.377 kb on + strand, within Shewana3_0244 at 276.417 kb on + strand, within Shewana3_0244 at 276.440 kb on + strand, within Shewana3_0244 at 276.461 kb on - strand, within Shewana3_0244 at 276.461 kb on - strand, within Shewana3_0244 at 276.466 kb on - strand at 276.606 kb on - strand, within Shewana3_0245 at 276.836 kb on - strand, within Shewana3_0245 at 276.958 kb on + strand, within Shewana3_0245 at 276.983 kb on + strand, within Shewana3_0245 at 276.991 kb on - strand, within Shewana3_0245 at 277.052 kb on - strand, within Shewana3_0245 at 277.078 kb on - strand, within Shewana3_0245 at 277.164 kb on - strand at 277.603 kb on - strand, within Shewana3_0246 at 277.624 kb on + strand, within Shewana3_0246 at 278.004 kb on - strand, within Shewana3_0247 at 278.085 kb on - strand, within Shewana3_0247
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 275,814 - Shewana3_0243 0.62 -0.4 275,846 - Shewana3_0243 0.68 -0.5 275,990 - -1.0 276,095 + +1.0 276,158 - Shewana3_0244 0.19 -0.5 276,186 + Shewana3_0244 0.26 -0.1 276,308 + Shewana3_0244 0.54 +0.2 276,377 + Shewana3_0244 0.69 -0.2 276,417 + Shewana3_0244 0.79 -0.9 276,440 + Shewana3_0244 0.84 -0.4 276,461 - Shewana3_0244 0.89 -0.0 276,461 - Shewana3_0244 0.89 +0.3 276,466 - +0.1 276,606 - Shewana3_0245 0.11 +0.8 276,836 - Shewana3_0245 0.49 +1.2 276,958 + Shewana3_0245 0.70 +0.7 276,983 + Shewana3_0245 0.74 -0.1 276,991 - Shewana3_0245 0.75 -0.4 277,052 - Shewana3_0245 0.85 -1.1 277,078 - Shewana3_0245 0.90 -0.2 277,164 - +0.1 277,603 - Shewana3_0246 0.61 +0.2 277,624 + Shewana3_0246 0.66 +1.1 278,004 - Shewana3_0247 0.42 -0.4 278,085 - Shewana3_0247 0.59 -0.8
Or see this region's nucleotide sequence