Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0166 and Shewana3_0167 are separated by 234 nucleotides Shewana3_0167 and Shewana3_0168 are separated by 119 nucleotides Shewana3_0168 and nudE are separated by 237 nucleotides nudE and Shewana3_0170 are separated by 70 nucleotides
Shewana3_0166: Shewana3_0166 - GCN5-related N-acetyltransferase (RefSeq), at 190,152 to 190,640
_0166
Shewana3_0167: Shewana3_0167 - phage tail collar domain-containing protein (RefSeq), at 190,875 to 191,510
_0167
Shewana3_0168: Shewana3_0168 - Ig family protein (RefSeq), at 191,630 to 202,264
_0168
Shewana3_0169: nudE - ADP-ribose diphosphatase NudE (RefSeq), at 202,502 to 203,053
nudE
Shewana3_0170: Shewana3_0170 - 3'(2'),5'-bisphosphate nucleotidase (RefSeq), at 203,124 to 203,936
_0170
Position (kb) 191
192
193
194
195
196
197
198
199
200
201
202
203 Strain fitness (log2 ratio) -2
-1
0
1 at 190.836 kb on + strand at 190.970 kb on + strand, within Shewana3_0167 at 191.034 kb on + strand, within Shewana3_0167 at 191.053 kb on + strand, within Shewana3_0167 at 191.244 kb on - strand, within Shewana3_0167 at 191.287 kb on - strand, within Shewana3_0167 at 191.365 kb on - strand, within Shewana3_0167 at 191.373 kb on + strand, within Shewana3_0167 at 191.373 kb on + strand, within Shewana3_0167 at 191.378 kb on + strand, within Shewana3_0167 at 191.381 kb on - strand, within Shewana3_0167 at 191.391 kb on - strand, within Shewana3_0167 at 191.407 kb on + strand, within Shewana3_0167 at 191.416 kb on - strand, within Shewana3_0167 at 191.422 kb on + strand, within Shewana3_0167 at 191.613 kb on + strand at 191.613 kb on + strand at 191.613 kb on + strand at 191.613 kb on + strand at 191.621 kb on - strand at 191.621 kb on - strand at 191.621 kb on - strand at 191.634 kb on - strand at 191.649 kb on - strand at 191.754 kb on + strand at 191.754 kb on + strand at 191.762 kb on - strand at 191.892 kb on + strand at 191.899 kb on + strand at 191.942 kb on + strand at 191.979 kb on + strand at 192.047 kb on + strand at 192.093 kb on + strand at 192.117 kb on - strand at 192.242 kb on + strand at 192.268 kb on + strand at 192.304 kb on + strand at 192.463 kb on - strand at 192.622 kb on - strand at 192.660 kb on + strand at 192.747 kb on - strand, within Shewana3_0168 at 192.747 kb on - strand, within Shewana3_0168 at 192.806 kb on - strand, within Shewana3_0168 at 192.806 kb on - strand, within Shewana3_0168 at 192.910 kb on - strand, within Shewana3_0168 at 193.013 kb on - strand, within Shewana3_0168 at 193.013 kb on - strand, within Shewana3_0168 at 193.013 kb on - strand, within Shewana3_0168 at 193.013 kb on - strand, within Shewana3_0168 at 193.028 kb on + strand, within Shewana3_0168 at 193.044 kb on + strand, within Shewana3_0168 at 193.052 kb on - strand, within Shewana3_0168 at 193.052 kb on - strand, within Shewana3_0168 at 193.271 kb on + strand, within Shewana3_0168 at 193.327 kb on + strand, within Shewana3_0168 at 193.426 kb on + strand, within Shewana3_0168 at 193.508 kb on + strand, within Shewana3_0168 at 193.508 kb on + strand, within Shewana3_0168 at 193.508 kb on + strand, within Shewana3_0168 at 193.508 kb on + strand, within Shewana3_0168 at 193.508 kb on + strand, within Shewana3_0168 at 193.516 kb on - strand, within Shewana3_0168 at 193.516 kb on - strand, within Shewana3_0168 at 193.516 kb on - strand, within Shewana3_0168 at 193.516 kb on - strand, within Shewana3_0168 at 193.516 kb on - strand, within Shewana3_0168 at 193.622 kb on + strand, within Shewana3_0168 at 193.630 kb on - strand, within Shewana3_0168 at 193.786 kb on - strand, within Shewana3_0168 at 193.838 kb on - strand, within Shewana3_0168 at 193.965 kb on - strand, within Shewana3_0168 at 194.115 kb on + strand, within Shewana3_0168 at 194.123 kb on - strand, within Shewana3_0168 at 194.161 kb on + strand, within Shewana3_0168 at 194.274 kb on + strand, within Shewana3_0168 at 194.462 kb on - strand, within Shewana3_0168 at 194.468 kb on - strand, within Shewana3_0168 at 194.783 kb on + strand, within Shewana3_0168 at 194.797 kb on + strand, within Shewana3_0168 at 195.063 kb on - strand, within Shewana3_0168 at 195.098 kb on + strand, within Shewana3_0168 at 195.106 kb on - strand, within Shewana3_0168 at 195.106 kb on - strand, within Shewana3_0168 at 195.228 kb on - strand, within Shewana3_0168 at 195.248 kb on - strand, within Shewana3_0168 at 195.271 kb on - strand, within Shewana3_0168 at 198.747 kb on + strand, within Shewana3_0168 at 198.787 kb on + strand, within Shewana3_0168 at 198.789 kb on + strand, within Shewana3_0168 at 198.811 kb on - strand, within Shewana3_0168 at 198.828 kb on + strand, within Shewana3_0168 at 198.836 kb on - strand, within Shewana3_0168 at 198.894 kb on + strand, within Shewana3_0168 at 199.097 kb on + strand, within Shewana3_0168 at 199.097 kb on + strand, within Shewana3_0168 at 199.105 kb on - strand, within Shewana3_0168 at 199.112 kb on - strand, within Shewana3_0168 at 199.137 kb on + strand, within Shewana3_0168 at 199.173 kb on - strand, within Shewana3_0168 at 199.300 kb on + strand, within Shewana3_0168 at 199.302 kb on + strand, within Shewana3_0168 at 199.307 kb on + strand, within Shewana3_0168 at 199.332 kb on + strand, within Shewana3_0168 at 199.332 kb on + strand, within Shewana3_0168 at 199.338 kb on - strand, within Shewana3_0168 at 199.338 kb on - strand, within Shewana3_0168 at 199.340 kb on - strand, within Shewana3_0168 at 199.340 kb on - strand, within Shewana3_0168 at 199.340 kb on - strand, within Shewana3_0168 at 199.340 kb on - strand, within Shewana3_0168 at 199.370 kb on + strand, within Shewana3_0168 at 199.441 kb on + strand, within Shewana3_0168 at 199.451 kb on - strand, within Shewana3_0168 at 199.451 kb on - strand, within Shewana3_0168 at 199.483 kb on + strand, within Shewana3_0168 at 199.483 kb on + strand, within Shewana3_0168 at 199.483 kb on + strand, within Shewana3_0168 at 199.483 kb on + strand, within Shewana3_0168 at 199.491 kb on - strand, within Shewana3_0168 at 199.540 kb on + strand, within Shewana3_0168 at 199.548 kb on - strand, within Shewana3_0168 at 199.725 kb on - strand, within Shewana3_0168 at 199.838 kb on - strand, within Shewana3_0168 at 199.953 kb on + strand, within Shewana3_0168 at 199.959 kb on + strand, within Shewana3_0168 at 199.998 kb on + strand, within Shewana3_0168 at 200.035 kb on - strand, within Shewana3_0168 at 200.133 kb on + strand, within Shewana3_0168 at 200.172 kb on + strand, within Shewana3_0168 at 200.177 kb on + strand, within Shewana3_0168 at 200.210 kb on + strand, within Shewana3_0168 at 200.380 kb on - strand, within Shewana3_0168 at 200.597 kb on + strand, within Shewana3_0168 at 200.604 kb on + strand, within Shewana3_0168 at 200.714 kb on + strand, within Shewana3_0168 at 200.721 kb on + strand, within Shewana3_0168 at 200.867 kb on + strand, within Shewana3_0168 at 200.994 kb on + strand, within Shewana3_0168 at 201.015 kb on - strand, within Shewana3_0168 at 201.153 kb on - strand, within Shewana3_0168 at 201.155 kb on + strand, within Shewana3_0168 at 201.194 kb on + strand, within Shewana3_0168 at 201.269 kb on - strand at 201.605 kb on + strand at 201.628 kb on - strand at 201.641 kb on + strand at 201.704 kb on - strand at 201.813 kb on - strand at 201.831 kb on - strand at 201.844 kb on + strand at 201.999 kb on + strand at 202.027 kb on + strand at 202.118 kb on + strand at 202.187 kb on + strand at 202.470 kb on - strand at 202.602 kb on - strand, within nudE at 202.803 kb on + strand, within nudE at 202.818 kb on + strand, within nudE at 202.818 kb on + strand, within nudE at 202.887 kb on + strand, within nudE at 202.978 kb on - strand, within nudE
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 190,836 + +0.4 190,970 + Shewana3_0167 0.15 -0.1 191,034 + Shewana3_0167 0.25 +0.1 191,053 + Shewana3_0167 0.28 -0.6 191,244 - Shewana3_0167 0.58 +0.5 191,287 - Shewana3_0167 0.65 +0.1 191,365 - Shewana3_0167 0.77 +1.0 191,373 + Shewana3_0167 0.78 +0.1 191,373 + Shewana3_0167 0.78 -0.4 191,378 + Shewana3_0167 0.79 +0.4 191,381 - Shewana3_0167 0.80 -0.8 191,391 - Shewana3_0167 0.81 +0.2 191,407 + Shewana3_0167 0.84 -0.3 191,416 - Shewana3_0167 0.85 -0.6 191,422 + Shewana3_0167 0.86 +0.2 191,613 + +0.2 191,613 + +0.5 191,613 + +0.6 191,613 + -0.2 191,621 - -1.2 191,621 - -0.3 191,621 - -0.1 191,634 - -1.9 191,649 - -1.7 191,754 + -0.3 191,754 + -1.0 191,762 - -0.3 191,892 + -0.3 191,899 + -0.8 191,942 + -0.4 191,979 + +0.1 192,047 + -0.2 192,093 + -0.1 192,117 - -2.5 192,242 + -0.5 192,268 + -0.3 192,304 + +0.9 192,463 - -0.5 192,622 - -0.8 192,660 + -1.4 192,747 - Shewana3_0168 0.11 +0.0 192,747 - Shewana3_0168 0.11 -0.1 192,806 - Shewana3_0168 0.11 +0.8 192,806 - Shewana3_0168 0.11 -0.4 192,910 - Shewana3_0168 0.12 +0.1 193,013 - Shewana3_0168 0.13 +0.8 193,013 - Shewana3_0168 0.13 +0.1 193,013 - Shewana3_0168 0.13 -0.4 193,013 - Shewana3_0168 0.13 -0.7 193,028 + Shewana3_0168 0.13 -1.5 193,044 + Shewana3_0168 0.13 +0.9 193,052 - Shewana3_0168 0.13 -0.0 193,052 - Shewana3_0168 0.13 +1.1 193,271 + Shewana3_0168 0.15 -1.5 193,327 + Shewana3_0168 0.16 -0.1 193,426 + Shewana3_0168 0.17 -1.3 193,508 + Shewana3_0168 0.18 +0.4 193,508 + Shewana3_0168 0.18 +0.2 193,508 + Shewana3_0168 0.18 +0.1 193,508 + Shewana3_0168 0.18 +0.1 193,508 + Shewana3_0168 0.18 -0.9 193,516 - Shewana3_0168 0.18 +0.0 193,516 - Shewana3_0168 0.18 -0.1 193,516 - Shewana3_0168 0.18 -0.1 193,516 - Shewana3_0168 0.18 -0.5 193,516 - Shewana3_0168 0.18 +0.5 193,622 + Shewana3_0168 0.19 +0.3 193,630 - Shewana3_0168 0.19 -1.6 193,786 - Shewana3_0168 0.20 -0.1 193,838 - Shewana3_0168 0.21 +1.0 193,965 - Shewana3_0168 0.22 -0.1 194,115 + Shewana3_0168 0.23 -0.7 194,123 - Shewana3_0168 0.23 +0.5 194,161 + Shewana3_0168 0.24 -0.6 194,274 + Shewana3_0168 0.25 -0.3 194,462 - Shewana3_0168 0.27 +0.0 194,468 - Shewana3_0168 0.27 +0.3 194,783 + Shewana3_0168 0.30 -0.0 194,797 + Shewana3_0168 0.30 +0.0 195,063 - Shewana3_0168 0.32 -0.3 195,098 + Shewana3_0168 0.33 -0.5 195,106 - Shewana3_0168 0.33 -0.1 195,106 - Shewana3_0168 0.33 +0.1 195,228 - Shewana3_0168 0.34 -0.9 195,248 - Shewana3_0168 0.34 +0.1 195,271 - Shewana3_0168 0.34 -0.0 198,747 + Shewana3_0168 0.67 +0.3 198,787 + Shewana3_0168 0.67 +0.2 198,789 + Shewana3_0168 0.67 +0.4 198,811 - Shewana3_0168 0.68 -0.3 198,828 + Shewana3_0168 0.68 -0.0 198,836 - Shewana3_0168 0.68 +1.1 198,894 + Shewana3_0168 0.68 -0.2 199,097 + Shewana3_0168 0.70 +0.3 199,097 + Shewana3_0168 0.70 -0.8 199,105 - Shewana3_0168 0.70 -0.2 199,112 - Shewana3_0168 0.70 -0.2 199,137 + Shewana3_0168 0.71 +0.0 199,173 - Shewana3_0168 0.71 -0.6 199,300 + Shewana3_0168 0.72 +0.2 199,302 + Shewana3_0168 0.72 +0.1 199,307 + Shewana3_0168 0.72 +0.0 199,332 + Shewana3_0168 0.72 -0.2 199,332 + Shewana3_0168 0.72 -0.9 199,338 - Shewana3_0168 0.72 -0.2 199,338 - Shewana3_0168 0.72 -0.0 199,340 - Shewana3_0168 0.72 +0.4 199,340 - Shewana3_0168 0.72 +0.6 199,340 - Shewana3_0168 0.72 -0.1 199,340 - Shewana3_0168 0.72 +0.3 199,370 + Shewana3_0168 0.73 +1.4 199,441 + Shewana3_0168 0.73 -0.0 199,451 - Shewana3_0168 0.74 +0.1 199,451 - Shewana3_0168 0.74 +0.6 199,483 + Shewana3_0168 0.74 +0.2 199,483 + Shewana3_0168 0.74 +1.1 199,483 + Shewana3_0168 0.74 -1.8 199,483 + Shewana3_0168 0.74 +1.7 199,491 - Shewana3_0168 0.74 +0.7 199,540 + Shewana3_0168 0.74 +0.3 199,548 - Shewana3_0168 0.74 -1.0 199,725 - Shewana3_0168 0.76 +0.3 199,838 - Shewana3_0168 0.77 -1.0 199,953 + Shewana3_0168 0.78 +0.2 199,959 + Shewana3_0168 0.78 +0.0 199,998 + Shewana3_0168 0.79 -0.3 200,035 - Shewana3_0168 0.79 -1.0 200,133 + Shewana3_0168 0.80 +0.0 200,172 + Shewana3_0168 0.80 +0.2 200,177 + Shewana3_0168 0.80 +0.3 200,210 + Shewana3_0168 0.81 +1.0 200,380 - Shewana3_0168 0.82 -0.3 200,597 + Shewana3_0168 0.84 +0.1 200,604 + Shewana3_0168 0.84 -0.1 200,714 + Shewana3_0168 0.85 +0.4 200,721 + Shewana3_0168 0.85 +1.2 200,867 + Shewana3_0168 0.87 +0.3 200,994 + Shewana3_0168 0.88 -1.5 201,015 - Shewana3_0168 0.88 +0.2 201,153 - Shewana3_0168 0.90 +0.7 201,155 + Shewana3_0168 0.90 +0.4 201,194 + Shewana3_0168 0.90 +0.3 201,269 - -0.8 201,605 + +0.6 201,628 - -0.5 201,641 + +0.1 201,704 - -0.8 201,813 - -0.8 201,831 - -0.8 201,844 + +0.4 201,999 + -0.5 202,027 + -0.6 202,118 + +0.4 202,187 + +0.6 202,470 - +0.2 202,602 - nudE Shewana3_0169 0.18 +1.1 202,803 + nudE Shewana3_0169 0.55 +0.8 202,818 + nudE Shewana3_0169 0.57 +0.1 202,818 + nudE Shewana3_0169 0.57 +0.8 202,887 + nudE Shewana3_0169 0.70 +0.2 202,978 - nudE Shewana3_0169 0.86 -2.1
Or see this region's nucleotide sequence