Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0113 and Shewana3_0114 are separated by 2 nucleotides Shewana3_0114 and Shewana3_0115 are separated by 124 nucleotides Shewana3_0115 and Shewana3_0116 are separated by 197 nucleotides
Shewana3_0113: Shewana3_0113 - hypothetical protein (RefSeq), at 129,604 to 130,224
_0113
Shewana3_0114: Shewana3_0114 - hypothetical protein (RefSeq), at 130,227 to 131,216
_0114
Shewana3_0115: Shewana3_0115 - hypothetical protein (RefSeq), at 131,341 to 131,982
_0115
Shewana3_0116: Shewana3_0116 - hypothetical protein (RefSeq), at 132,180 to 132,563
_0116
Position (kb) 130
131
132 Strain fitness (log2 ratio) -1
0
1 at 129.235 kb on + strand at 129.343 kb on + strand at 129.420 kb on - strand at 129.420 kb on - strand at 129.512 kb on + strand at 129.534 kb on + strand at 129.539 kb on + strand at 129.580 kb on + strand at 129.663 kb on + strand at 129.671 kb on - strand, within Shewana3_0113 at 129.816 kb on + strand, within Shewana3_0113 at 129.957 kb on + strand, within Shewana3_0113 at 129.965 kb on - strand, within Shewana3_0113 at 130.117 kb on + strand, within Shewana3_0113 at 130.238 kb on + strand at 130.481 kb on + strand, within Shewana3_0114 at 130.691 kb on - strand, within Shewana3_0114 at 130.698 kb on - strand, within Shewana3_0114 at 130.699 kb on + strand, within Shewana3_0114 at 130.699 kb on + strand, within Shewana3_0114 at 130.699 kb on + strand, within Shewana3_0114 at 130.699 kb on + strand, within Shewana3_0114 at 130.699 kb on + strand, within Shewana3_0114 at 130.934 kb on + strand, within Shewana3_0114 at 130.993 kb on + strand, within Shewana3_0114 at 130.993 kb on + strand, within Shewana3_0114 at 131.037 kb on + strand, within Shewana3_0114 at 131.181 kb on + strand at 131.183 kb on + strand at 131.183 kb on + strand at 131.191 kb on - strand at 131.346 kb on - strand at 131.636 kb on - strand, within Shewana3_0115 at 131.870 kb on + strand, within Shewana3_0115 at 131.878 kb on - strand, within Shewana3_0115 at 131.889 kb on + strand, within Shewana3_0115 at 131.897 kb on - strand, within Shewana3_0115 at 131.903 kb on - strand, within Shewana3_0115 at 131.951 kb on - strand at 132.100 kb on + strand at 132.163 kb on + strand at 132.176 kb on + strand at 132.183 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 129,235 + +0.4 129,343 + -0.5 129,420 - -1.6 129,420 - +0.2 129,512 + +0.5 129,534 + -0.0 129,539 + -0.2 129,580 + +0.2 129,663 + -0.1 129,671 - Shewana3_0113 0.11 -0.2 129,816 + Shewana3_0113 0.34 +0.8 129,957 + Shewana3_0113 0.57 +0.8 129,965 - Shewana3_0113 0.58 +0.3 130,117 + Shewana3_0113 0.83 +0.3 130,238 + +0.5 130,481 + Shewana3_0114 0.26 +0.7 130,691 - Shewana3_0114 0.47 -0.7 130,698 - Shewana3_0114 0.48 -0.2 130,699 + Shewana3_0114 0.48 +0.2 130,699 + Shewana3_0114 0.48 +0.2 130,699 + Shewana3_0114 0.48 +0.0 130,699 + Shewana3_0114 0.48 +0.1 130,699 + Shewana3_0114 0.48 +0.7 130,934 + Shewana3_0114 0.71 +0.8 130,993 + Shewana3_0114 0.77 +0.4 130,993 + Shewana3_0114 0.77 +0.3 131,037 + Shewana3_0114 0.82 +0.2 131,181 + -0.4 131,183 + -0.1 131,183 + +1.1 131,191 - -1.1 131,346 - -0.0 131,636 - Shewana3_0115 0.46 +0.3 131,870 + Shewana3_0115 0.82 -0.4 131,878 - Shewana3_0115 0.84 +0.9 131,889 + Shewana3_0115 0.85 -0.6 131,897 - Shewana3_0115 0.87 -0.8 131,903 - Shewana3_0115 0.88 +0.2 131,951 - -0.9 132,100 + +1.1 132,163 + +0.2 132,176 + +0.4 132,183 - +0.6
Or see this region's nucleotide sequence